Team:BYU Provo/Team OxyR/Week13

From 2011.igem.org

Revision as of 00:13, 29 September 2011 by MattBiggs (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Team BYU Provo

BYU Provo
 

Contents

18 July 2011

Matt and Rob came in and set up colony PCR to check the OxyR-Med ligations. Rob was the master of boiling/patching colonies.

19 July 2011

PCR results from July 18th:

GelPic19July.JPG

20 July 2011

Purified plasmids from the most recent round of transformations (using the colonies that passed the colony PCR test).

21 July 2011

Performed another ligation trying the katG promoter again.

22 July 2011

Plates that Matt started (the ligation from July 21st) were only pinpricks in the morning. Rob allowed them to grow a bit longer.

23 July 2011

Mackay set up a colony PCR checking the KatG/OxyR-Medium construct again (the one that hadn't been working).