Team:Amsterdam/9 September 2011
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Revision as of 10:34, 21 September 2011 by B.Stringer (Talk | contribs)
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09-09-2011
Constructs
An 3 hour ligation was performed at 16°C to make the following constructs:
- K538130-Cpn60
- K206000-K538101
Backbone transferring
Digestion of different parts to tranfer to the Chloramphenicol backbone:
- All genes
- The constructs we got thus far
Gel purification
The different parts were put on gel and purified with the qiaquick gel extraction kit. The following parts showed a good result:
- Cpn10
- K538104
- CspC
- K538101
- Cpn60
Ligation
An 3 hour ligation was performed at 16°C to transfer the following parts (from 08-09) into the Chloramphenicol backbone:
- K538101
- Cpn60
- K538120
Transformation
After 3 hours of ligation all samples were tranformed into an E.coli Top 10 strain.