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From 2011.igem.org

Contents 1 08-09-2011 1.1 Constructs 1.1.1 Digestion 1.1.2 Backbone transferring

08-09-2011

Constructs

The following parts were miniprepped:

• Cpn10
• Cpn60
• CspC
• K538100
• K538101
• K538104
• K538120
• K538130
• K538200
• Shednak
• R0010
• K206000

Digestion

Constructs making using the 2a assembly method. Digestion of different parts to construct the following assemblies:

• R0010+ B0034 + CspC
• R0010+ B0034 + Cpn60
• R0010+ B0034 + Shednak
• K206000+ B0034 + CspC
• K206000+ B0034 + Cpn60
• K206000+ B0034 + Shednak
  

These assemblies were ligated ON at 16°C.

Backbone transferring

Digestion of different parts to tranfer to the Chloramphenicol backbone:

• All genes
• The constructs we've miniprepped today

These parts were ligated into the Chloramphenicol backbone ON at 16°C.