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From 2011.igem.org

Contents 1 Ideas for modelling 1.1 Steady state model of promoters 1.2 The cold chock promoter 1.3 Cryobrick 1.4 Enzyme activity

Ideas for modelling

Brainstorm session with Douwe Molenaar and Isoude Kuijper

Steady state model of promoters

• Information needed for model
  • Stability
    • Transcription
      • Measuring with qPCR
    • Translation
      • Fluorescence
  • Growth rate
• Information needed for all promoters
  • Inducible promoter
    • IPTG promoter is also dependable on a lactose receptor/transporter
  • Cold shock promoter
• Temperature dependent
  • The inducible promoters are also concentration dependent
    • Need to look into literature for already available information

The cold chock promoter

• How does this promoter work?

Cryobrick

• What concentration is needed?
  • How many targets do the chaperone proteins have?

Enzyme activity

• Enzyme activity is also temperature dependent