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Team:Amsterdam/6 July 2011
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==Steady state model of promoters== | ==Steady state model of promoters== | ||
Revision as of 12:00, 6 July 2011
Contents |
Ideas for modelling
Brainstorm session with Douwe Molenaar and Isoude Kuijper
Steady state model of promoters
- Information needed for model
- Stability
- Transcription
- Measuring with qPCR
- Transcription
- Translation
- Fluorescence
- Growth rate
- Stability
- Information needed for all promoters
- Inducible promoter
- IPTG promoter is also dependable on a lactose receptor/transporter
- Cold shock promoter
- Inducible promoter
- Temperature dependent
- The inducible promoters are also concentration dependent
- Need to look into literature for already available information
- The inducible promoters are also concentration dependent
The cold chock promoter
- How does this promoter work?
Cryobrick
- What concentration is needed?
- How many targets do the chaperone proteins have?
Enzyme activity
- Enzyme activity is also temperature dependent
