29-08-2011
Growth curves
Measuring growth curves off our wild type (TOP 10), at 4, 8, 15, and 21°C.
Construct check
The ON samples were minprepped, digested with EcorI and PstI; and checked on gel.
New constructs
The constructs were digested to add extra parts. The following constructs were made using the assembly protocol from Delft 2010:
- R0010+ B0034 + CspC
- R0010+ B0034 + Cpn10
- R0010+ B0034 + Cpn60
- R0010+ B0034 + Shednak
- K206000+ B0034 + CspC
- K206000+ B0034 + Cpn10
- K206000+ B0034 + Cpn60
- K206000+ B0034 + Shednak