Team:Amsterdam/25 August 2011
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==Growth curves== | ==Growth curves== | ||
- | Measuring growth curves off our wild type (TOP 10), at 37, 30 and 25°C. (Monday 4, 8, 15 and | + | Measuring growth curves off our wild type (TOP 10), at 37, 30 and 25°C. (Monday 4, 8, 15 and 21°C will be tested)<br> |
Also tested if the growth curve was different if the E.coli was stirred with a bean, or shacked in a stove. | Also tested if the growth curve was different if the E.coli was stirred with a bean, or shacked in a stove. | ||
+ | ==Constructs== | ||
+ | 2 different assembly methods were used: 3a assembly and the protocol from Delft 2010. This was done to make the following constructs: | ||
+ | *B0034-Cpn10 | ||
+ | *B0034-Cpn60 | ||
+ | *B0034-CspC | ||
+ | *R0010-B0034 | ||
+ | *K26000-B0034 | ||
+ | These parts were digested and afterwards a ligation was performed ON at 16 degrees Celcius. | ||
{{:Team:Amsterdam/Footer}} | {{:Team:Amsterdam/Footer}} |
Latest revision as of 10:32, 21 September 2011
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25-08-2011
Growth curves
Measuring growth curves off our wild type (TOP 10), at 37, 30 and 25°C. (Monday 4, 8, 15 and 21°C will be tested)
Also tested if the growth curve was different if the E.coli was stirred with a bean, or shacked in a stove.
Constructs
2 different assembly methods were used: 3a assembly and the protocol from Delft 2010. This was done to make the following constructs:
- B0034-Cpn10
- B0034-Cpn60
- B0034-CspC
- R0010-B0034
- K26000-B0034
These parts were digested and afterwards a ligation was performed ON at 16 degrees Celcius.