Team:Amsterdam/12 September 2011
From 2011.igem.org
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12-09-2011
Growth curves
Measuring growth curves off our first construct!! We measure Cpn10 with a lacz in a ampicillin backbone. As control we take WT (top 10). Today we measured at 37, 30 and 25°C. (Tomorrow 4, 8 and at Wednesday 15 and 21°C will be tested)
Constructs
Colony PCR
A colony PCR has been performed on 28 different colonies from the transformation. The samples were checked on agarose gel. This showed good results for the following constructs in Chloramphenicol backbone:
- Cpn60
- K538101
- K538104
- Cpn10
New Constructs
Constructs making using the 2a assembly method. Digestion of different parts to construct the following assemblies:
- R0010+ B0034 + CspC
- R0010+ B0034 + Cpn60
- R0010+ B0034 + Shednak
- K206000+ B0034 + CspC
- K206000+ B0034 + Cpn60
- K206000+ B0034 + Shednak
Backbone transferring
Digestion of different parts to tranfer to the Chloramphenicol backbone:
- All genes
- All constructs to thus far