Team:Amsterdam/12 September 2011

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12-09-2011

Growth curves

Measuring growth curves off our first construct!! We measure Cpn10 with a lacz in a ampicillin backbone. As control we take WT (top 10). Today we measured at 37, 30 and 25°C. (Tomorrow 4, 8 and at Wednesday 15 and 21°C will be tested)

Constructs

Colony PCR

A colony PCR has been performed on 28 different colonies from the transformation. The samples were checked on agarose gel. This showed good results for the following constructs in Chloramphenicol backbone:

  • Cpn60
  • K538101
  • K538104
  • Cpn10

New Constructs

Constructs making using the 2a assembly method. Digestion of different parts to construct the following assemblies:

  • R0010+ B0034 + CspC
  • R0010+ B0034 + Cpn60
  • R0010+ B0034 + Shednak
  • K206000+ B0034 + CspC
  • K206000+ B0034 + Cpn60
  • K206000+ B0034 + Shednak


Backbone transferring

Digestion of different parts to tranfer to the Chloramphenicol backbone:

  • All genes
  • All constructs to thus far