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Team:Amsterdam/12 September 2011
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==Growth curves== | ==Growth curves== | ||
Measuring growth curves off our first construct!! We measure Cpn10 with a lacz in a ampicillin backbone. As control we take WT (top 10). Today we measured at 37, 30 and 25°C. (Tomorrow 4, 8 and at Wednesday 15 and 21°C will be tested)<br> | Measuring growth curves off our first construct!! We measure Cpn10 with a lacz in a ampicillin backbone. As control we take WT (top 10). Today we measured at 37, 30 and 25°C. (Tomorrow 4, 8 and at Wednesday 15 and 21°C will be tested)<br> | ||
| + | |||
| + | ==Constructs== | ||
| + | ===Colony PCR=== | ||
| + | A colony PCR has been performed on 28 different colonies from the transformation. The samples were checked on agarose gel. This showed good results for the following constructs in Chloramphenicol backbone: | ||
| + | *Cpn60 | ||
| + | *K538101 | ||
| + | *K538104 | ||
| + | *Cpn10 | ||
| + | |||
| + | ===New Constructs=== | ||
| + | Constructs making using the 2a assembly method. | ||
| + | Digestion of different parts to construct the following assemblies: | ||
| + | *R0010+ B0034 + CspC | ||
| + | *R0010+ B0034 + Cpn60 | ||
| + | *R0010+ B0034 + Shednak | ||
| + | *K206000+ B0034 + CspC | ||
| + | *K206000+ B0034 + Cpn60 | ||
| + | *K206000+ B0034 + Shednak <br> | ||
| + | |||
| + | |||
| + | ===Backbone transferring=== | ||
| + | Digestion of different parts to tranfer to the Chloramphenicol backbone: | ||
| + | *All genes | ||
| + | *All constructs to thus far | ||
{{:Team:Amsterdam/Footer}} | {{:Team:Amsterdam/Footer}} | ||
Latest revision as of 10:34, 21 September 2011
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12-09-2011
Growth curves
Measuring growth curves off our first construct!! We measure Cpn10 with a lacz in a ampicillin backbone. As control we take WT (top 10). Today we measured at 37, 30 and 25°C. (Tomorrow 4, 8 and at Wednesday 15 and 21°C will be tested)
Constructs
Colony PCR
A colony PCR has been performed on 28 different colonies from the transformation. The samples were checked on agarose gel. This showed good results for the following constructs in Chloramphenicol backbone:
- Cpn60
- K538101
- K538104
- Cpn10
New Constructs
Constructs making using the 2a assembly method. Digestion of different parts to construct the following assemblies:
- R0010+ B0034 + CspC
- R0010+ B0034 + Cpn60
- R0010+ B0034 + Shednak
- K206000+ B0034 + CspC
- K206000+ B0034 + Cpn60
- K206000+ B0034 + Shednak
Backbone transferring
Digestion of different parts to tranfer to the Chloramphenicol backbone:
- All genes
- All constructs to thus far
