Team:Amsterdam/11 July 2011

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11-07-2011 | Meeting 18

Workplan

  • A platereader is available
    • Only meassurments can be done at RT and 37 degrees Celcius
  • No difference can be seen between promoter and protein folding at cold temperatures
    • Maybe with qPCR?
      • We need to look into this, it is expensive, will it work at low temperatures?
  • Can we use GFP at cold temperatures for promoter characterisation?
    • Search for other useful proteins like GFP
  • Synthesized genes are ordered

Labwork

  • Ensembly of 2 plasmids will be done
    • First miniprep, then test on gel
      • Make glycerol stock of the good colonies

Funding

  • Kickstarter had some delay, but will be online next week
  • VU/UVA funding is still in progress
  • Isogen sponsored us a photospectrometer and a PCR, to borrow it for some time

Modelling

  • How to model the promoter activity
  • Meassure OD and fluorescence

Human Outreach/PR

  • EHBU isn't useful for us
    • We won't be there
    • We will concentrate on Nemo/Naturalis
  • Ad valvas, we can have a short story in September

Labwork

  • Plates with transformants were counted
  • Multiple colonies were grown ON 37 degrees celsius in liquid culture