"
Sunday, July 31
From 2011.igem.org
(Created page with " {{LabReportBmore}}") |
|||
| Line 1: | Line 1: | ||
| + | Once again tried tranformation into competent cells. Used 2 protocols 1st protocol from New England Biodabs High Efficiency Protocol for C2987 cells | ||
| + | Added 1 ul of each of material from tubes 1, 3, 2B, 2, 1B, (dot or scribble),4A, 5A, 5B to 50 ul of competent cells (was not enough competent cells to do all tube samples | ||
| + | Added control PNC19 to one of the competent cell vials, and put no dna into the second competent cell vial. | ||
| + | Left on ice for 30 minutes. heat shocked tubes 2 at a time for 45 secs. Placed on ice for 5 minutes. be | ||
| + | Pipetted 950 ul of SOC into each tube | ||
| + | put in warm room shaker, shook for 1 hour | ||
| - | {{LabReportBmore}} | + | Used a Protocol from Tom, from Chung, Niemela, and Miller "One-step preparation of competent Escherichia coli: Transformation and storage of bacterial cells in the same solution," Proc Natl. Acad. Sci Vol 86, pp 2172-2175, April 1989 {{LabReportBmore}} |
Revision as of 21:42, 31 July 2011
Once again tried tranformation into competent cells. Used 2 protocols 1st protocol from New England Biodabs High Efficiency Protocol for C2987 cells Added 1 ul of each of material from tubes 1, 3, 2B, 2, 1B, (dot or scribble),4A, 5A, 5B to 50 ul of competent cells (was not enough competent cells to do all tube samples Added control PNC19 to one of the competent cell vials, and put no dna into the second competent cell vial.
Left on ice for 30 minutes. heat shocked tubes 2 at a time for 45 secs. Placed on ice for 5 minutes. be
Pipetted 950 ul of SOC into each tube put in warm room shaker, shook for 1 hour
Used a Protocol from Tom, from Chung, Niemela, and Miller "One-step preparation of competent Escherichia coli: Transformation and storage of bacterial cells in the same solution," Proc Natl. Acad. Sci Vol 86, pp 2172-2175, April 1989 Team:Baltimore/Notebook
Hours
