http://2011.igem.org/wiki/index.php?title=Special:Contributions/Fazilet&feed=atom&limit=50&target=Fazilet&year=&month=
2011.igem.org - User contributions [en]
2024-03-29T07:05:56Z
From 2011.igem.org
MediaWiki 1.16.0
http://2011.igem.org/Team:Fatih_Turkey/Fazilet
Team:Fatih Turkey/Fazilet
2011-10-29T03:54:03Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Fazilet Güler</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Medical Student<br />
(3rd Year)<br />
</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="float: left; width: 220px; border-radius: 10px; position: absolute; top: 50px; left: 0; background: #EEE; border: solid 3px #666;"><br />
<ol> <br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<ol><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Alperen">Alperen Güver</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Bet%C3%BCl">Betül Günbey</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/HSY">Hasan Yasin Soylu</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Fazilet">Faziet Güler</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Kenan">Kenan Güzel</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Hale">Halenur Güngördü</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Mustafa">Mustafa S. Elitok</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/secil">Seçil Taşhan</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/ozan">Ozan Sami Bayrak</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/tulin">Tülin Serap Yılmaz</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/yckr">Yasin Çakar</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/buraks">Burak Şimşek</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/cihan">Cihan Taştan</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/muradiye">Muradiye Acar</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/esrahoca">Esra Gündüz</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/herdamar">Kadir Demircan</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/kadirh">Hüsamettin Erdamar</a></li><br />
<br />
</ol><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
<br />
</ol><br />
</div><br />
<br />
<div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;"><br />
<br><br />
<br><br />
<br><br />
<img src="https://static.igem.org/mediawiki/2011/7/7a/Team-fazilet.jpg" style="width: 160px;float: right; margin: 10px;"><br />
<li><b>Mail:</b> fazilet.guler@gmail.com</li><br />
<li><b>Experiment:</b> Sequence analising</li><br />
<li><b>Lab Device:</b> Face Mask:)</li><br />
<li><b>Motto:</b> But!</li><br />
<li><b>Getto:</b> iGEM lab</li><br />
<li><b>Best wishes:</b> I hope, everyone can have a greate team like us.</li><br />
<br />
<br />
<br><br />
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Fazilet
Team:Fatih Turkey/Fazilet
2011-10-29T03:53:41Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Fazilet Güler</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Medical Student<br />
(3rd Year)<br />
</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="float: left; width: 220px; border-radius: 10px; position: absolute; top: 50px; left: 0; background: #EEE; border: solid 3px #666;"><br />
<ol> <br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<ol><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Alperen">Alperen Güver</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Bet%C3%BCl">Betül Günbey</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/HSY">Hasan Yasin Soylu</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Fazilet">Faziet Güler</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Kenan">Kenan Güzel</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Hale">Halenur Güngördü</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Mustafa">Mustafa S. Elitok</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/secil">Seçil Taşhan</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/ozan">Ozan Sami Bayrak</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/tulin">Tülin Serap Yılmaz</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/yckr">Yasin Çakar</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/buraks">Burak Şimşek</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/cihan">Cihan Taştan</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/muradiye">Muradiye Acar</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/esrahoca">Esra Gündüz</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/herdamar">Kadir Demircan</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/kadirh">Hüsamettin Erdamar</a></li><br />
<br />
</ol><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
<br />
</ol><br />
</div><br />
<br />
<div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;"><br />
<br><br />
<br><br />
<br><br />
<img src="https://static.igem.org/mediawiki/2011/7/7a/Team-fazilet.jpg" style="width: 160px;float: right; margin: 10px;"><br />
<li><b>Mail:</b> fazilet.guler@gmail.com</li><br />
<li><b>Experiment:</b> Sequence analising</li><br />
<li><b>Lab Device:</b>Face Mask:)</li><br />
<li><b>Motto:</b> But!</li><br />
<li><b>Getto:</b> iGEM lab</li><br />
<li><b>Best wishes:</b> I hope, everyone can have a greate team like us.</li><br />
<br />
<br />
<br><br />
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Notebook13
Team:Fatih Turkey/Notebook13
2011-10-29T02:57:17Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Lab Garage</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Notebook</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<br />
<style><br />
#haftalar li{<br />
width: 20px;<br />
text-align: center;<br />
margin: 0 20px;<br />
float: left;<br />
list-style: none;<br />
cursor: pointer;<br />
font-size: 14px;<br />
font-weight:bold;<br />
border-radius: 6px;<br />
}<br />
<br />
#haftalar li:hover{<br />
background: #CCC;<br />
}<br />
<br />
#haftalar li.active{<br />
background: #CCC;<br />
}<br />
</style><br />
<br />
<br />
<div style="margin-left: 40px;"><br />
<span style="float: left;">Week: </span><br />
<ul id="haftalar"><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">1</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook2">2</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook3">3</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook4">4</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook5">5</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook6">6</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook7">7</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook8">8</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook9">9</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook10">10</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook11">11</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook12">12</a></li><br />
<li class="active"><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook13">13</a></li><br />
</ul><br />
</div><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="width: 840px; border: solid 3px #666; border-radius: 10px; padding-left: 30px;padding-right: 30px; margin:center "><br />
<br><br />
<p> We made like MİC(minimum inhibitory concentration) experiment which is using been for antibiotic because of finding minimum concentration of LALF that get inhibition of growing e.coli.But we didn’t added sequence which help us in purifying of protein like His-tag which we were synthesising LALF .So we used supernatant of B.subtilis which produced LALF instead of pure LALF protein. <br /><br />
<b> 23.10.2011</b><br />
<br><br />
We used culture of e. coli whose OD is 0,4 with GFP.50 ul culture of e.coli and 3-50 ul supernatant of B.subtilis with LALF and without LALF were put into all wells.Lb with Chl was added by completing total volume to 100 ul.<br /><br />
<b> 24.10.2011 </b><br />
<br><br />
We used culture of e.coli whose OD is 0,4 with GFP .25 ul culture of e.coli and 75 ul supernatant of B.subtilis with LALF and without LALF were put into all wells.Lb with Chl was added by completing total volume to 100 ul.<br /><br />
<b> 25.10.2011</b><br />
<br> <br />
We used culture of e.coli whose OD is 0,1 with GFP .25 ul culture of e.coli and 75 ul supernatant of B.subtilis with LALF and without LALF were put into all wells.Lb with Chl was added by completing total volume to 100 ul.<br /><br />
<b>26.10.2011</b><br />
<br> <br />
We used culture of e.coli whose OD is 0,2 with GFP .25 ul culture of e.coli and 75 ul supernatant of B.subtilis with LALF and without LALF were put into all wells. Lb with Chl was added by completing total volume to 100 ul.<br /><br />
<b>27.10.2011</b><br />
<br> <br />
The experiment which was made for observing to supernatant of B.subtilis with LALF effect on liquid culture of e.coli.<br /><br />
6 ml LB with chl ,2ul e.coli which was growthed overnight ;10,100,1000,2000 ul B.subtilis supernatant with LALF and without LALF was added.<br /><br />
B.subtilis supernatant with LALF and without LALF was completed to 2 ml with Lb has chl.<br /><br />
Measure of OD was made at 0-2-4-6-8 hours in 600 and 405 nm.<br /><br />
29.10.2011</b><br />
E.coli with RFP was used.<br /><br />
1. We used 50ul e.coli culture whose OD came 0,1 with RFP and 50 ul LALF.<br /><br />
2. We used 25ul e.coli culture whose OD came 0,1 with RFP and 75ul LALF.<br /><br />
3.We used 90ul e.coli culture whose OD came 0,1 with RFP and 10ul LALF.<br /><br />
<br /><br />
4. We used 25ul e.coli culture whose OD came 0,2 with RFP and 75 ul LALF.<br /><br />
5. We used 10ul e.coli culture whose OD came 0,2 with RFP and 90 ul LALF.<br /><br />
6. We used 25ul e.coli culture whose OD came 0,3 with RFP and 75ul LALF.<br /><br />
7. We used 10ul e.coli culture whose OD came 0,3 with RFP and 90ul LALF.<br /><br />
8. We used 10ul e.coli culture whose OD came 0,4 with RFP and 90ul LALF.</p><br />
<p> </p><br />
<br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Notebook13
Team:Fatih Turkey/Notebook13
2011-10-29T02:54:03Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Lab Garage</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Notebook</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<br />
<style><br />
#haftalar li{<br />
width: 20px;<br />
text-align: center;<br />
margin: 0 20px;<br />
float: left;<br />
list-style: none;<br />
cursor: pointer;<br />
font-size: 14px;<br />
font-weight:bold;<br />
border-radius: 6px;<br />
}<br />
<br />
#haftalar li:hover{<br />
background: #CCC;<br />
}<br />
<br />
#haftalar li.active{<br />
background: #CCC;<br />
}<br />
</style><br />
<br />
<br />
<div style="margin-left: 40px;"><br />
<span style="float: left;">Week: </span><br />
<ul id="haftalar"><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">1</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook2">2</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook3">3</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook4">4</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook5">5</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook6">6</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook7">7</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook8">8</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook9">9</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook10">10</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook11">11</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook12">12</a></li><br />
<li class="active"><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook13">13</a></li><br />
</ul><br />
</div><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="width: 840px; border: solid 3px #666; border-radius: 10px; padding-left: 30px;padding-right: 30px; margin:center "><br />
<br />
<p> We made like MİC(minimum inhibitory concentration) experiment which is using been for antibiotic because of finding minimum concentration of LALF that get inhibition of growing e.coli.But we didn’t added sequence which help us in purifying of protein like His-tag which we were synthesising LALF .So we used supernatant of B.subtilis which produced LALF instead of pure LALF protein. <br /><br />
23.10.2011<br /><br />
We used culture of e. coli whose OD is 0,4 with GFP.50 ul culture of e.coli and 3-50 ul supernatant of B.subtilis with LALF and without LALF were put into all wells.Lb with Chl was added by completing total volume to 100 ul.<br /><br />
24.10.2011<br /><br />
We used culture of e.coli whose OD is 0,4 with GFP .25 ul culture of e.coli and 75 ul supernatant of B.subtilis with LALF and without LALF were put into all wells.Lb with Chl was added by completing total volume to 100 ul.<br /><br />
25.10.2011<br /><br />
We used culture of e.coli whose OD is 0,1 with GFP .25 ul culture of e.coli and 75 ul supernatant of B.subtilis with LALF and without LALF were put into all wells.Lb with Chl was added by completing total volume to 100 ul.<br /><br />
26.10.2011<br /><br />
We used culture of e.coli whose OD is 0,2 with GFP .25 ul culture of e.coli and 75 ul supernatant of B.subtilis with LALF and without LALF were put into all wells. Lb with Chl was added by completing total volume to 100 ul.<br /><br />
27.10.2011<br /><br />
The experiment which was made for observing to supernatant of B.subtilis with LALF effect on liquid culture of e.coli.<br /><br />
6 ml LB with chl ,2ul e.coli which was growthed overnight ;10,100,1000,2000 ul B.subtilis supernatant with LALF and without LALF was added.<br /><br />
B.subtilis supernatant with LALF and without LALF was completed to 2 ml with Lb has chl.<br /><br />
Measure of OD was made at 0-2-4-6-8 hours in 600 and 405 nm.<br /><br />
29.10.2011<br /><br />
E.coli with RFP was used.<br /><br />
1. We used 50ul e.coli culture whose OD came 0,1 with RFP and 50 ul LALF.<br /><br />
2. We used 25ul e.coli culture whose OD came 0,1 with RFP and 75ul LALF.<br /><br />
3.We used 90ul e.coli culture whose OD came 0,1 with RFP and 10ul LALF.<br /><br />
<br /><br />
4. We used 25ul e.coli culture whose OD came 0,2 with RFP and 75 ul LALF.<br /><br />
5. We used 10ul e.coli culture whose OD came 0,2 with RFP and 90 ul LALF.<br /><br />
6. We used 25ul e.coli culture whose OD came 0,3 with RFP and 75ul LALF.<br /><br />
7. We used 10ul e.coli culture whose OD came 0,3 with RFP and 90ul LALF.<br /><br />
8. We used 10ul e.coli culture whose OD came 0,4 with RFP and 90ul LALF.</p><br />
<p> </p><br />
<br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Sporocide
Team:Fatih Turkey/Sporocide
2011-10-29T02:37:15Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporiocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Sporicide</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<center> <b >EFFECTIVE SOLUTION TO BIG DANGER: SPORICIDE </b></center><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/0/02/DSC03586_-_Kopya.JPG" weight="300"></center></p><br />
<br />
<br><br />
<p><center><em>Left: fenton reagent + aqueous dissolved oxygen<br /><br />
Right: fenton reagent + distilled water </em></center> <br /><br />
The reason that we chose B.subtilis in our project is the LPS binding character of LALF. Gram positive bacteria such as B.subtilis have no LPS layer on their cell wall. This obligation of choosing alternative bacterium leads to another problem: B.subtilis has the ability to form endospores. <br /><br />
B.subtilis, normally, has no pathogenic character unless it forms endospores under some circumstances like high temperature, limit pH levels or deoxygenized media. The inhalation of endospores is very dangerous and must be avoided in the case of study on B.subtilis.<br /><br />
Decontamination of biological endospores is very important considering the necessary of the safety in synthetic biology laboratories. Especially in iGEM, teams may need alternative creatures to plan and perform their projects properly like in our project. Because of this, we began to look through the web and hope to find a solution for this safety problem. Fortunately, some specific dilutions are characterized to solve possible safety problems in order to assemble aseptic surfaces. <strong>Fenton reagents</strong>, which kill septic microorganisms with mechanism of hydroxyl radicals, were traditionally being used. However; its major ingredient, hydrogen peroxide, was examined and was compared with aqueous dissolved oxygen about their capability of killing the spores. (J. B. Cross, 2003)1<br />
<br /><br />
It is said that in addition to aqueous dissolved oxygen, cupric chloride, ascorbic acid, sodium chloride and a little sulfactant dilution which are used as modified Fenton reagent are more effective on endospores of gram positive bacteria. In contrast; classic Fenton reagent, which possess hydrogen peroxide and copper ion, shows that gram negative bacteria are more vulnerable to this formulation. The mechanism of these dilutions is not well-characterized; however, comparing with other dilutions that include specific oxidizing agents such as ozone, some differences in the images that are gained from effected bacteria are indicated. It is deduced that modified Fenton reagent affects the inside mechanism of the bacteria; although the dilutions with ozone damages the coat of the bacteria. After formation of hydroxyl radicals within the spore, these highly reactive molecules attack either the DNA or enzymes necessary for the spore conversion to a bacterial cell. Further investigations of ion transport into spores and oxidation-reductions with in spores will be needed to determine precisely the mechanism of spore kill under these conditions.<br /><br />
The effectiveness of modified Fenton Reagent and the advantages/disadvantages of different Fenton Reagent solutions are characterized during our experiments. For more information about Fenton Reagent experiments, please visit <u>Fenton Reagent Experiments</u> page.<br /><br />
Another problem we faced was that the sporicide can also kill gram negative bacteria in their non-endospore forms. Our LALF protein is there for this reason; but we should be sure that gram negative bacteria are terminated just because of LALF protein. To achieve this, we decided to extract the oxygen ingradient which is the main component of killing mechanism in the Fenton reagent. Thus, hydroxyl radical mechanism of their agents would not work and only mechanism on the surface as terminator would be our anti-LPS factor against the gram negative bacteria, especially E.coli in our project.<br /><br />
Considering the importance of safe and secure labs in synthetic biology, especially in iGEM, prospective projects including B.subtilis may confront such problems. The need of sporicide will increase in the next iGEM competitions. To have safer labs, we recommend our chemical decontaminator to all iGEM teams.</p><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/3/38/Ecolidfr.png" weight="300"></center></p><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/4/4c/Ecoliofr.png" weight="300"></center></p><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/2/2f/Bsofr.png" weight="300"></center></p><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/2/2f/Bsofr.png" weight="300"></center></p><br />
<p><strong>DISCUSSION</strong><br /><br />
As a conclusion, we observed <strong>distilled water</strong> included Fenton Reagent (DFR) is more effective than <strong>oxygenated water</strong> included Fenton Reagent (OFR) against E.coli. When 25 uL OFR and DFR have been added in E.coli cultures, 82% of DFR treated culture was dead and 47% of OFR treated culture was dead. We did not see any colony which included 50 uL and more volumes of DFR. In contrast, after 75 uL and more addition of OFR, no colony formation has been observed.</p><br />
<p>On the other hand, we observed OFR is more effective than DFR against Bacillus subtilis. When 10 uL OFR and DFR have been added in Bacillus subtilis cultures, 38% of DFR treated culture was dead and 98.3% of OFR treated culture was dead. Not only the 25 uL OFR treated but also 25 uL DFR treated culture was dead.</p><br />
<p>If we want to compare E.coli and B. subtilis, we can say that Fenton Reagent application is more fatal for Bacillus subtilis. Both 25 uL OFR and DFR treated Bacillus subtilis culture was dead but 25 uL OFR and DFR treated E.coli culture was still alive.</p><br />
<br />
<br><br />
<p>1 Killing of Bacillus Spores by Aqueous Dissolved Oxygen, Ascorbic Acid, and Copper Ions; J. B. Cross, R. P. Currier, D. J. Torraco, L. A. Vanderberg, G. L. Wagner, and P. D. Gladen Chemistry Division1 and Biosciences Division,2 Los Alamos National Laboratory, Los Alamos, New Mexico 87545 </p><br />
<br><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Game_Rules
Team:Fatih Turkey/Game Rules
2011-10-29T02:33:16Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<br />
<div style="float:left;margin-right:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/game"><h2> Game </h2></a></div><br />
<br />
<br />
<img src="https://static.igem.org/mediawiki/2011/a/ad/Oyun1.png" /><hr><br />
<br />
<h1><b>Game Properties</b></h1><br />
<h2 style="text-decoration: underline">Materials</h2><br />
<ul><br />
<li>One dice</li><br />
<li>Three Bacillus Subtilis, three Escherichia Coli pawns</li><br />
<li>9 character cards, 96 basic gene cards and 96 gene synthesis cards</li><br />
<li>Gameboard</li><br />
<li>“Lysogenic Cycle” and turn standby icons</li><br />
</ul><br />
<br><br />
<h2 style="text-decoration: underline">Game Plan</h2><br />
<ul><br />
<li>At the beginning, character cards are distributed to all players randomly. Players receive their pawns that match with their character cards.</li><br />
<li>The players with same character assemble teams of kinds.</li><br />
<li>One member of each team rolls dice to determine which team is going to start first. Then, members of the first team also roll dice. Team member that has the biggest number on the dice is the first player.</li><br />
<li>Game continues counterclockwise as from the first player.</li><br />
<li>Each player goes forward times of the number on the dice.</li><br />
<li>The first player that reaches the last golden box is the winner.</li><br />
</ul><br />
<hr><br />
<h1><b>General Rules</b></h1><br />
<br />
<h2 style="text-decoration: underline">Survival</h2><br />
<br />
In the case that player can survive on a box only by using a gene card, that player must use his/her card. Use of cards that are not “vital” is optional.<br><br><br />
<br />
<h2 style="text-decoration: underline">Returning to Checkpoint</h2><br />
<br />
Bacteria can be killed by some effects. In the game, any player that is “killed” must resume the game from last checkpoint box. There are two checkpoint box and a start box. Players that could not reach the first checkpoint must return to start box if necessary.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Conjugation</h2><br />
<br />
If two members of a team meet on the same box, these players can exchange their basic gene cards.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gang-o-Bang</h2><br />
<br />
If B. Subtilis and E.coli members meet in the same box, E. coli kills B. Subtilis player. Then, B. Subtilis goes back to the last checkpoint or start point. But; if B. Subtilis has the “Anti-LPS card”, B. Subtilis survives and E.coli dies.<br><br><hr><br />
<h1><b>Gameboard Rules</b></h1><br><br />
<br />
<strong>Antibiotic Boxes</strong><br><br />
<br />
All players except Virus players are killed and returns to the last checkpoint box unless the basic gene cards or gene synthesis cards of the player have no resistance to that antibiotic. Note that there are four kinds of antibiotic boxes: Ampicilin, chloramphenicol, kanamycin and tetracyclin.<br><br><br />
<br />
<strong>Checkpoints</strong><br><br />
<br />
When player passes a checkpoint box, next time that player is killed by an effect; player resumes the game from these boxes.<br><br><br />
<br />
<strong>Restriction Enzymes</strong><br><br />
<br />
If one of the restriction sites that are denoted on the basic gene cardsmatches with the restriction enzyme, that card is put end of the card deck. If the restriction site of the character card matches, the player resumes the game from the last checkpoint box. Note that there are four restriction enzymes: EcoR1, Pst1, Spe1 and Xba1.<br><br><br />
<hr><br />
<h1><b>Cards</b></h1><br />
<br />
<h2 style="text-decoration: underline;">Character Cards</h2><br />
<br />
Each player will have a character card that gives the player some advantages and disadvantages depend on the type of the card and the situation. There are two types of character cards: Escherichia coli, Bacillus subtilis.<br><br />
<br />
On the character cards, there are two boxes for special circumstances: Genome restriction sites and super power.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Restriction site box also remains on the character cards. If the character card matches with the enzyme, player returns to last checkpoint box.<br><br><br />
<br />
<strong>Super Power</strong><br><br />
<br />
Each kind of character card has its own super power that gives them some advantages in the game.<br><br />
<br />
Bacillus Subtilis has the power of producing endospores. Thus, it is resistant to some sterilization techniques, in other word, some boxes on the board do not effect. These boxes are marked with sheriff star.<br><br />
<br />
The genome of Escherichia coli is known very well; thus E.coli has an advantage about getting gene cards easily. When an E.coli player stops on any checkpoint square (start box is not included), player draws one additional basic gene card. (Note that when player is killed and returns to last checkpoint, same rule exists.)<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gene Cards</h2><br />
<br />
There are two kinds of gene cards: Basic gene and gene synthesis cards. Only difference between these two kinds is that basic gene cards have restriction sites additionally.<br><br><br />
<br />
<strong>Anti-LPS Biofilm</strong><br><br />
<br />
This card allows B.subtilis players to kill E.coli – which means E.coli player must continue the game from the last checkpoint box – in the case of meeting on any box.<br><br><br />
<br />
<strong>Chemotaxis</strong><br><br />
<br />
This card allows playersto go forward two more boxes on the “Glucose Rich Medium” box. In contrast, on the “Hydrogen peroxide” box, players go two steps backward.<br><br><br />
<br />
<strong>Deep Freeze</strong><br><br />
<br />
This card allows the player not to wait on the “-80 C<sup>o</sup>media” box for one turn.<br><br><br />
<br />
<strong>Encapsulation</strong><br><br />
<br />
This card allows E.coli player to be treated like B.subtilis player for that turn. In another word, E.coli character card becomes B.subtilis character card for the turn that this card is used.<br><br><br />
<br />
<strong>Fenton Reagent</strong><br><br />
<br />
The player can put this card on any box on the board. Any player stops on that box, returns to last checkpoint box.<br><br><br />
<br />
<strong>iGEM</strong><br><br />
<br />
Player looks up for any gene synthesis card from its deck and exchange it with this card. (iGEM card goes under the basic gene card deck.) Gene synthesis card deck is then shuffled. This card can only be used at the beginning of a turn.<br><br><br />
<br />
<strong>Joker</strong><br><br />
<br />
This card can be treated as any kind of basic gene card. Note that this card also has all restriction sites and all kinds of antibiotic resistance.<br><br><br />
<br />
<strong>Lightening</strong><br><br />
<br />
This card allows the player not to be affected on the “Ampicilin antibiotic” box.<br><br><br />
<br />
<strong>Lysogenic Cycle</strong><br><br />
<br />
Player with this card is not affected by “Virus” box. Instead of it, player receives a “Lysogenic Cycle” marker and continues the game. If the player with that marker stops on “Virus Environment?” square, that player returns to last checkpoint. (Note that after being killed, “Lysogenic Cycle” marker is lost.)<br><br><br />
<br />
<strong>Second Chance</strong><br><br />
<br />
If a player is killed, that player resumes the game without returning to last checkpoint via using this card.<br><br><br />
<br />
<strong>Take a Photo</strong><br><br />
<br />
This card allows the player who has reached Canvas Town square to go on two more squares.<br><br />
<br />
On the gene cards there are three boxes for special circumstances: Promoter, restriction site and antibiotic resistance.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Genes have their own cleavage zones. These zones are called “restriction sites”. On the card, restriction site box shows which restriction enzyme is able to cut the gene and make it unable to be used. There are four enzymes that have their own boxes on the game board: EcoR1, Spe1, Pst1 and Xba1. Some cards may have restriction enzymes more than one.<br><br />
<br />
When player stops on a restriction site box on the board; restriction site boxes on the cards must be checked if any of them fits. The card matches with the restriction enzyme goes end of the card deck.<br><br />
<br />
Note that gene synthesis cards do not have restriction site box.<br><br><br />
<br />
<strong>Antibiotic Resistance</strong><br><br />
<br />
This box shows that the player who owns it cannot be harmed by that type of antibiotic. There are four antibiotic boxes on the game board: Chloramphenicol, ampicilin, kanamycin and tetracyclin.<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/6/60/Oyun2.png" /><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Game_Rules
Team:Fatih Turkey/Game Rules
2011-10-29T02:32:49Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<br />
<div style="float:left;margin-right:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/game"><h2> Game</h2></a></div><br />
<br />
<br />
<img src="https://static.igem.org/mediawiki/2011/a/ad/Oyun1.png" /><hr><br />
<br />
<h1><b>Game Properties</b></h1><br />
<h2 style="text-decoration: underline">Materials</h2><br />
<ul><br />
<li>One dice</li><br />
<li>Three Bacillus Subtilis, three Escherichia Coli pawns</li><br />
<li>9 character cards, 96 basic gene cards and 96 gene synthesis cards</li><br />
<li>Gameboard</li><br />
<li>“Lysogenic Cycle” and turn standby icons</li><br />
</ul><br />
<br><br />
<h2 style="text-decoration: underline">Game Plan</h2><br />
<ul><br />
<li>At the beginning, character cards are distributed to all players randomly. Players receive their pawns that match with their character cards.</li><br />
<li>The players with same character assemble teams of kinds.</li><br />
<li>One member of each team rolls dice to determine which team is going to start first. Then, members of the first team also roll dice. Team member that has the biggest number on the dice is the first player.</li><br />
<li>Game continues counterclockwise as from the first player.</li><br />
<li>Each player goes forward times of the number on the dice.</li><br />
<li>The first player that reaches the last golden box is the winner.</li><br />
</ul><br />
<hr><br />
<h1><b>General Rules</b></h1><br />
<br />
<h2 style="text-decoration: underline">Survival</h2><br />
<br />
In the case that player can survive on a box only by using a gene card, that player must use his/her card. Use of cards that are not “vital” is optional.<br><br><br />
<br />
<h2 style="text-decoration: underline">Returning to Checkpoint</h2><br />
<br />
Bacteria can be killed by some effects. In the game, any player that is “killed” must resume the game from last checkpoint box. There are two checkpoint box and a start box. Players that could not reach the first checkpoint must return to start box if necessary.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Conjugation</h2><br />
<br />
If two members of a team meet on the same box, these players can exchange their basic gene cards.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gang-o-Bang</h2><br />
<br />
If B. Subtilis and E.coli members meet in the same box, E. coli kills B. Subtilis player. Then, B. Subtilis goes back to the last checkpoint or start point. But; if B. Subtilis has the “Anti-LPS card”, B. Subtilis survives and E.coli dies.<br><br><hr><br />
<h1><b>Gameboard Rules</b></h1><br><br />
<br />
<strong>Antibiotic Boxes</strong><br><br />
<br />
All players except Virus players are killed and returns to the last checkpoint box unless the basic gene cards or gene synthesis cards of the player have no resistance to that antibiotic. Note that there are four kinds of antibiotic boxes: Ampicilin, chloramphenicol, kanamycin and tetracyclin.<br><br><br />
<br />
<strong>Checkpoints</strong><br><br />
<br />
When player passes a checkpoint box, next time that player is killed by an effect; player resumes the game from these boxes.<br><br><br />
<br />
<strong>Restriction Enzymes</strong><br><br />
<br />
If one of the restriction sites that are denoted on the basic gene cardsmatches with the restriction enzyme, that card is put end of the card deck. If the restriction site of the character card matches, the player resumes the game from the last checkpoint box. Note that there are four restriction enzymes: EcoR1, Pst1, Spe1 and Xba1.<br><br><br />
<hr><br />
<h1><b>Cards</b></h1><br />
<br />
<h2 style="text-decoration: underline;">Character Cards</h2><br />
<br />
Each player will have a character card that gives the player some advantages and disadvantages depend on the type of the card and the situation. There are two types of character cards: Escherichia coli, Bacillus subtilis.<br><br />
<br />
On the character cards, there are two boxes for special circumstances: Genome restriction sites and super power.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Restriction site box also remains on the character cards. If the character card matches with the enzyme, player returns to last checkpoint box.<br><br><br />
<br />
<strong>Super Power</strong><br><br />
<br />
Each kind of character card has its own super power that gives them some advantages in the game.<br><br />
<br />
Bacillus Subtilis has the power of producing endospores. Thus, it is resistant to some sterilization techniques, in other word, some boxes on the board do not effect. These boxes are marked with sheriff star.<br><br />
<br />
The genome of Escherichia coli is known very well; thus E.coli has an advantage about getting gene cards easily. When an E.coli player stops on any checkpoint square (start box is not included), player draws one additional basic gene card. (Note that when player is killed and returns to last checkpoint, same rule exists.)<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gene Cards</h2><br />
<br />
There are two kinds of gene cards: Basic gene and gene synthesis cards. Only difference between these two kinds is that basic gene cards have restriction sites additionally.<br><br><br />
<br />
<strong>Anti-LPS Biofilm</strong><br><br />
<br />
This card allows B.subtilis players to kill E.coli – which means E.coli player must continue the game from the last checkpoint box – in the case of meeting on any box.<br><br><br />
<br />
<strong>Chemotaxis</strong><br><br />
<br />
This card allows playersto go forward two more boxes on the “Glucose Rich Medium” box. In contrast, on the “Hydrogen peroxide” box, players go two steps backward.<br><br><br />
<br />
<strong>Deep Freeze</strong><br><br />
<br />
This card allows the player not to wait on the “-80 C<sup>o</sup>media” box for one turn.<br><br><br />
<br />
<strong>Encapsulation</strong><br><br />
<br />
This card allows E.coli player to be treated like B.subtilis player for that turn. In another word, E.coli character card becomes B.subtilis character card for the turn that this card is used.<br><br><br />
<br />
<strong>Fenton Reagent</strong><br><br />
<br />
The player can put this card on any box on the board. Any player stops on that box, returns to last checkpoint box.<br><br><br />
<br />
<strong>iGEM</strong><br><br />
<br />
Player looks up for any gene synthesis card from its deck and exchange it with this card. (iGEM card goes under the basic gene card deck.) Gene synthesis card deck is then shuffled. This card can only be used at the beginning of a turn.<br><br><br />
<br />
<strong>Joker</strong><br><br />
<br />
This card can be treated as any kind of basic gene card. Note that this card also has all restriction sites and all kinds of antibiotic resistance.<br><br><br />
<br />
<strong>Lightening</strong><br><br />
<br />
This card allows the player not to be affected on the “Ampicilin antibiotic” box.<br><br><br />
<br />
<strong>Lysogenic Cycle</strong><br><br />
<br />
Player with this card is not affected by “Virus” box. Instead of it, player receives a “Lysogenic Cycle” marker and continues the game. If the player with that marker stops on “Virus Environment?” square, that player returns to last checkpoint. (Note that after being killed, “Lysogenic Cycle” marker is lost.)<br><br><br />
<br />
<strong>Second Chance</strong><br><br />
<br />
If a player is killed, that player resumes the game without returning to last checkpoint via using this card.<br><br><br />
<br />
<strong>Take a Photo</strong><br><br />
<br />
This card allows the player who has reached Canvas Town square to go on two more squares.<br><br />
<br />
On the gene cards there are three boxes for special circumstances: Promoter, restriction site and antibiotic resistance.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Genes have their own cleavage zones. These zones are called “restriction sites”. On the card, restriction site box shows which restriction enzyme is able to cut the gene and make it unable to be used. There are four enzymes that have their own boxes on the game board: EcoR1, Spe1, Pst1 and Xba1. Some cards may have restriction enzymes more than one.<br><br />
<br />
When player stops on a restriction site box on the board; restriction site boxes on the cards must be checked if any of them fits. The card matches with the restriction enzyme goes end of the card deck.<br><br />
<br />
Note that gene synthesis cards do not have restriction site box.<br><br><br />
<br />
<strong>Antibiotic Resistance</strong><br><br />
<br />
This box shows that the player who owns it cannot be harmed by that type of antibiotic. There are four antibiotic boxes on the game board: Chloramphenicol, ampicilin, kanamycin and tetracyclin.<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/6/60/Oyun2.png" /><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Game_Rules
Team:Fatih Turkey/Game Rules
2011-10-29T02:32:16Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<br />
<div style="float:left;margin-right:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/game"><h2>Game Intrudiction</h2></a></div><br />
<br />
<br />
<img src="https://static.igem.org/mediawiki/2011/a/ad/Oyun1.png" /><hr><br />
<br />
<h1><b>Game Properties</b></h1><br />
<h2 style="text-decoration: underline">Materials</h2><br />
<ul><br />
<li>One dice</li><br />
<li>Three Bacillus Subtilis, three Escherichia Coli pawns</li><br />
<li>9 character cards, 96 basic gene cards and 96 gene synthesis cards</li><br />
<li>Gameboard</li><br />
<li>“Lysogenic Cycle” and turn standby icons</li><br />
</ul><br />
<br><br />
<h2 style="text-decoration: underline">Game Plan</h2><br />
<ul><br />
<li>At the beginning, character cards are distributed to all players randomly. Players receive their pawns that match with their character cards.</li><br />
<li>The players with same character assemble teams of kinds.</li><br />
<li>One member of each team rolls dice to determine which team is going to start first. Then, members of the first team also roll dice. Team member that has the biggest number on the dice is the first player.</li><br />
<li>Game continues counterclockwise as from the first player.</li><br />
<li>Each player goes forward times of the number on the dice.</li><br />
<li>The first player that reaches the last golden box is the winner.</li><br />
</ul><br />
<hr><br />
<h1><b>General Rules</b></h1><br />
<br />
<h2 style="text-decoration: underline">Survival</h2><br />
<br />
In the case that player can survive on a box only by using a gene card, that player must use his/her card. Use of cards that are not “vital” is optional.<br><br><br />
<br />
<h2 style="text-decoration: underline">Returning to Checkpoint</h2><br />
<br />
Bacteria can be killed by some effects. In the game, any player that is “killed” must resume the game from last checkpoint box. There are two checkpoint box and a start box. Players that could not reach the first checkpoint must return to start box if necessary.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Conjugation</h2><br />
<br />
If two members of a team meet on the same box, these players can exchange their basic gene cards.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gang-o-Bang</h2><br />
<br />
If B. Subtilis and E.coli members meet in the same box, E. coli kills B. Subtilis player. Then, B. Subtilis goes back to the last checkpoint or start point. But; if B. Subtilis has the “Anti-LPS card”, B. Subtilis survives and E.coli dies.<br><br><hr><br />
<h1><b>Gameboard Rules</b></h1><br><br />
<br />
<strong>Antibiotic Boxes</strong><br><br />
<br />
All players except Virus players are killed and returns to the last checkpoint box unless the basic gene cards or gene synthesis cards of the player have no resistance to that antibiotic. Note that there are four kinds of antibiotic boxes: Ampicilin, chloramphenicol, kanamycin and tetracyclin.<br><br><br />
<br />
<strong>Checkpoints</strong><br><br />
<br />
When player passes a checkpoint box, next time that player is killed by an effect; player resumes the game from these boxes.<br><br><br />
<br />
<strong>Restriction Enzymes</strong><br><br />
<br />
If one of the restriction sites that are denoted on the basic gene cardsmatches with the restriction enzyme, that card is put end of the card deck. If the restriction site of the character card matches, the player resumes the game from the last checkpoint box. Note that there are four restriction enzymes: EcoR1, Pst1, Spe1 and Xba1.<br><br><br />
<hr><br />
<h1><b>Cards</b></h1><br />
<br />
<h2 style="text-decoration: underline;">Character Cards</h2><br />
<br />
Each player will have a character card that gives the player some advantages and disadvantages depend on the type of the card and the situation. There are two types of character cards: Escherichia coli, Bacillus subtilis.<br><br />
<br />
On the character cards, there are two boxes for special circumstances: Genome restriction sites and super power.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Restriction site box also remains on the character cards. If the character card matches with the enzyme, player returns to last checkpoint box.<br><br><br />
<br />
<strong>Super Power</strong><br><br />
<br />
Each kind of character card has its own super power that gives them some advantages in the game.<br><br />
<br />
Bacillus Subtilis has the power of producing endospores. Thus, it is resistant to some sterilization techniques, in other word, some boxes on the board do not effect. These boxes are marked with sheriff star.<br><br />
<br />
The genome of Escherichia coli is known very well; thus E.coli has an advantage about getting gene cards easily. When an E.coli player stops on any checkpoint square (start box is not included), player draws one additional basic gene card. (Note that when player is killed and returns to last checkpoint, same rule exists.)<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gene Cards</h2><br />
<br />
There are two kinds of gene cards: Basic gene and gene synthesis cards. Only difference between these two kinds is that basic gene cards have restriction sites additionally.<br><br><br />
<br />
<strong>Anti-LPS Biofilm</strong><br><br />
<br />
This card allows B.subtilis players to kill E.coli – which means E.coli player must continue the game from the last checkpoint box – in the case of meeting on any box.<br><br><br />
<br />
<strong>Chemotaxis</strong><br><br />
<br />
This card allows playersto go forward two more boxes on the “Glucose Rich Medium” box. In contrast, on the “Hydrogen peroxide” box, players go two steps backward.<br><br><br />
<br />
<strong>Deep Freeze</strong><br><br />
<br />
This card allows the player not to wait on the “-80 C<sup>o</sup>media” box for one turn.<br><br><br />
<br />
<strong>Encapsulation</strong><br><br />
<br />
This card allows E.coli player to be treated like B.subtilis player for that turn. In another word, E.coli character card becomes B.subtilis character card for the turn that this card is used.<br><br><br />
<br />
<strong>Fenton Reagent</strong><br><br />
<br />
The player can put this card on any box on the board. Any player stops on that box, returns to last checkpoint box.<br><br><br />
<br />
<strong>iGEM</strong><br><br />
<br />
Player looks up for any gene synthesis card from its deck and exchange it with this card. (iGEM card goes under the basic gene card deck.) Gene synthesis card deck is then shuffled. This card can only be used at the beginning of a turn.<br><br><br />
<br />
<strong>Joker</strong><br><br />
<br />
This card can be treated as any kind of basic gene card. Note that this card also has all restriction sites and all kinds of antibiotic resistance.<br><br><br />
<br />
<strong>Lightening</strong><br><br />
<br />
This card allows the player not to be affected on the “Ampicilin antibiotic” box.<br><br><br />
<br />
<strong>Lysogenic Cycle</strong><br><br />
<br />
Player with this card is not affected by “Virus” box. Instead of it, player receives a “Lysogenic Cycle” marker and continues the game. If the player with that marker stops on “Virus Environment?” square, that player returns to last checkpoint. (Note that after being killed, “Lysogenic Cycle” marker is lost.)<br><br><br />
<br />
<strong>Second Chance</strong><br><br />
<br />
If a player is killed, that player resumes the game without returning to last checkpoint via using this card.<br><br><br />
<br />
<strong>Take a Photo</strong><br><br />
<br />
This card allows the player who has reached Canvas Town square to go on two more squares.<br><br />
<br />
On the gene cards there are three boxes for special circumstances: Promoter, restriction site and antibiotic resistance.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Genes have their own cleavage zones. These zones are called “restriction sites”. On the card, restriction site box shows which restriction enzyme is able to cut the gene and make it unable to be used. There are four enzymes that have their own boxes on the game board: EcoR1, Spe1, Pst1 and Xba1. Some cards may have restriction enzymes more than one.<br><br />
<br />
When player stops on a restriction site box on the board; restriction site boxes on the cards must be checked if any of them fits. The card matches with the restriction enzyme goes end of the card deck.<br><br />
<br />
Note that gene synthesis cards do not have restriction site box.<br><br><br />
<br />
<strong>Antibiotic Resistance</strong><br><br />
<br />
This box shows that the player who owns it cannot be harmed by that type of antibiotic. There are four antibiotic boxes on the game board: Chloramphenicol, ampicilin, kanamycin and tetracyclin.<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/6/60/Oyun2.png" /><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Game_Rules
Team:Fatih Turkey/Game Rules
2011-10-29T02:31:38Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<br />
<div style="float:left;margin-right:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/game"><h2>Game Int.</h2></a></div><br />
<br />
<br />
<img src="https://static.igem.org/mediawiki/2011/a/ad/Oyun1.png" /><hr><br />
<br />
<h1><b>Game Properties</b></h1><br />
<h2 style="text-decoration: underline">Materials</h2><br />
<ul><br />
<li>One dice</li><br />
<li>Three Bacillus Subtilis, three Escherichia Coli pawns</li><br />
<li>9 character cards, 96 basic gene cards and 96 gene synthesis cards</li><br />
<li>Gameboard</li><br />
<li>“Lysogenic Cycle” and turn standby icons</li><br />
</ul><br />
<br><br />
<h2 style="text-decoration: underline">Game Plan</h2><br />
<ul><br />
<li>At the beginning, character cards are distributed to all players randomly. Players receive their pawns that match with their character cards.</li><br />
<li>The players with same character assemble teams of kinds.</li><br />
<li>One member of each team rolls dice to determine which team is going to start first. Then, members of the first team also roll dice. Team member that has the biggest number on the dice is the first player.</li><br />
<li>Game continues counterclockwise as from the first player.</li><br />
<li>Each player goes forward times of the number on the dice.</li><br />
<li>The first player that reaches the last golden box is the winner.</li><br />
</ul><br />
<hr><br />
<h1><b>General Rules</b></h1><br />
<br />
<h2 style="text-decoration: underline">Survival</h2><br />
<br />
In the case that player can survive on a box only by using a gene card, that player must use his/her card. Use of cards that are not “vital” is optional.<br><br><br />
<br />
<h2 style="text-decoration: underline">Returning to Checkpoint</h2><br />
<br />
Bacteria can be killed by some effects. In the game, any player that is “killed” must resume the game from last checkpoint box. There are two checkpoint box and a start box. Players that could not reach the first checkpoint must return to start box if necessary.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Conjugation</h2><br />
<br />
If two members of a team meet on the same box, these players can exchange their basic gene cards.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gang-o-Bang</h2><br />
<br />
If B. Subtilis and E.coli members meet in the same box, E. coli kills B. Subtilis player. Then, B. Subtilis goes back to the last checkpoint or start point. But; if B. Subtilis has the “Anti-LPS card”, B. Subtilis survives and E.coli dies.<br><br><hr><br />
<h1><b>Gameboard Rules</b></h1><br><br />
<br />
<strong>Antibiotic Boxes</strong><br><br />
<br />
All players except Virus players are killed and returns to the last checkpoint box unless the basic gene cards or gene synthesis cards of the player have no resistance to that antibiotic. Note that there are four kinds of antibiotic boxes: Ampicilin, chloramphenicol, kanamycin and tetracyclin.<br><br><br />
<br />
<strong>Checkpoints</strong><br><br />
<br />
When player passes a checkpoint box, next time that player is killed by an effect; player resumes the game from these boxes.<br><br><br />
<br />
<strong>Restriction Enzymes</strong><br><br />
<br />
If one of the restriction sites that are denoted on the basic gene cardsmatches with the restriction enzyme, that card is put end of the card deck. If the restriction site of the character card matches, the player resumes the game from the last checkpoint box. Note that there are four restriction enzymes: EcoR1, Pst1, Spe1 and Xba1.<br><br><br />
<hr><br />
<h1><b>Cards</b></h1><br />
<br />
<h2 style="text-decoration: underline;">Character Cards</h2><br />
<br />
Each player will have a character card that gives the player some advantages and disadvantages depend on the type of the card and the situation. There are two types of character cards: Escherichia coli, Bacillus subtilis.<br><br />
<br />
On the character cards, there are two boxes for special circumstances: Genome restriction sites and super power.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Restriction site box also remains on the character cards. If the character card matches with the enzyme, player returns to last checkpoint box.<br><br><br />
<br />
<strong>Super Power</strong><br><br />
<br />
Each kind of character card has its own super power that gives them some advantages in the game.<br><br />
<br />
Bacillus Subtilis has the power of producing endospores. Thus, it is resistant to some sterilization techniques, in other word, some boxes on the board do not effect. These boxes are marked with sheriff star.<br><br />
<br />
The genome of Escherichia coli is known very well; thus E.coli has an advantage about getting gene cards easily. When an E.coli player stops on any checkpoint square (start box is not included), player draws one additional basic gene card. (Note that when player is killed and returns to last checkpoint, same rule exists.)<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gene Cards</h2><br />
<br />
There are two kinds of gene cards: Basic gene and gene synthesis cards. Only difference between these two kinds is that basic gene cards have restriction sites additionally.<br><br><br />
<br />
<strong>Anti-LPS Biofilm</strong><br><br />
<br />
This card allows B.subtilis players to kill E.coli – which means E.coli player must continue the game from the last checkpoint box – in the case of meeting on any box.<br><br><br />
<br />
<strong>Chemotaxis</strong><br><br />
<br />
This card allows playersto go forward two more boxes on the “Glucose Rich Medium” box. In contrast, on the “Hydrogen peroxide” box, players go two steps backward.<br><br><br />
<br />
<strong>Deep Freeze</strong><br><br />
<br />
This card allows the player not to wait on the “-80 C<sup>o</sup>media” box for one turn.<br><br><br />
<br />
<strong>Encapsulation</strong><br><br />
<br />
This card allows E.coli player to be treated like B.subtilis player for that turn. In another word, E.coli character card becomes B.subtilis character card for the turn that this card is used.<br><br><br />
<br />
<strong>Fenton Reagent</strong><br><br />
<br />
The player can put this card on any box on the board. Any player stops on that box, returns to last checkpoint box.<br><br><br />
<br />
<strong>iGEM</strong><br><br />
<br />
Player looks up for any gene synthesis card from its deck and exchange it with this card. (iGEM card goes under the basic gene card deck.) Gene synthesis card deck is then shuffled. This card can only be used at the beginning of a turn.<br><br><br />
<br />
<strong>Joker</strong><br><br />
<br />
This card can be treated as any kind of basic gene card. Note that this card also has all restriction sites and all kinds of antibiotic resistance.<br><br><br />
<br />
<strong>Lightening</strong><br><br />
<br />
This card allows the player not to be affected on the “Ampicilin antibiotic” box.<br><br><br />
<br />
<strong>Lysogenic Cycle</strong><br><br />
<br />
Player with this card is not affected by “Virus” box. Instead of it, player receives a “Lysogenic Cycle” marker and continues the game. If the player with that marker stops on “Virus Environment?” square, that player returns to last checkpoint. (Note that after being killed, “Lysogenic Cycle” marker is lost.)<br><br><br />
<br />
<strong>Second Chance</strong><br><br />
<br />
If a player is killed, that player resumes the game without returning to last checkpoint via using this card.<br><br><br />
<br />
<strong>Take a Photo</strong><br><br />
<br />
This card allows the player who has reached Canvas Town square to go on two more squares.<br><br />
<br />
On the gene cards there are three boxes for special circumstances: Promoter, restriction site and antibiotic resistance.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Genes have their own cleavage zones. These zones are called “restriction sites”. On the card, restriction site box shows which restriction enzyme is able to cut the gene and make it unable to be used. There are four enzymes that have their own boxes on the game board: EcoR1, Spe1, Pst1 and Xba1. Some cards may have restriction enzymes more than one.<br><br />
<br />
When player stops on a restriction site box on the board; restriction site boxes on the cards must be checked if any of them fits. The card matches with the restriction enzyme goes end of the card deck.<br><br />
<br />
Note that gene synthesis cards do not have restriction site box.<br><br><br />
<br />
<strong>Antibiotic Resistance</strong><br><br />
<br />
This box shows that the player who owns it cannot be harmed by that type of antibiotic. There are four antibiotic boxes on the game board: Chloramphenicol, ampicilin, kanamycin and tetracyclin.<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/6/60/Oyun2.png" /><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Game_Rules
Team:Fatih Turkey/Game Rules
2011-10-29T02:30:22Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<div style="float:left;margin-right:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/game"><h2>Game</h2></a></div><br />
<img src="https://static.igem.org/mediawiki/2011/a/ad/Oyun1.png" /><hr><br />
<br />
<h1><b>Game Properties</b></h1><br />
<h2 style="text-decoration: underline">Materials</h2><br />
<ul><br />
<li>One dice</li><br />
<li>Three Bacillus Subtilis, three Escherichia Coli pawns</li><br />
<li>9 character cards, 96 basic gene cards and 96 gene synthesis cards</li><br />
<li>Gameboard</li><br />
<li>“Lysogenic Cycle” and turn standby icons</li><br />
</ul><br />
<br><br />
<h2 style="text-decoration: underline">Game Plan</h2><br />
<ul><br />
<li>At the beginning, character cards are distributed to all players randomly. Players receive their pawns that match with their character cards.</li><br />
<li>The players with same character assemble teams of kinds.</li><br />
<li>One member of each team rolls dice to determine which team is going to start first. Then, members of the first team also roll dice. Team member that has the biggest number on the dice is the first player.</li><br />
<li>Game continues counterclockwise as from the first player.</li><br />
<li>Each player goes forward times of the number on the dice.</li><br />
<li>The first player that reaches the last golden box is the winner.</li><br />
</ul><br />
<hr><br />
<h1><b>General Rules</b></h1><br />
<br />
<h2 style="text-decoration: underline">Survival</h2><br />
<br />
In the case that player can survive on a box only by using a gene card, that player must use his/her card. Use of cards that are not “vital” is optional.<br><br><br />
<br />
<h2 style="text-decoration: underline">Returning to Checkpoint</h2><br />
<br />
Bacteria can be killed by some effects. In the game, any player that is “killed” must resume the game from last checkpoint box. There are two checkpoint box and a start box. Players that could not reach the first checkpoint must return to start box if necessary.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Conjugation</h2><br />
<br />
If two members of a team meet on the same box, these players can exchange their basic gene cards.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gang-o-Bang</h2><br />
<br />
If B. Subtilis and E.coli members meet in the same box, E. coli kills B. Subtilis player. Then, B. Subtilis goes back to the last checkpoint or start point. But; if B. Subtilis has the “Anti-LPS card”, B. Subtilis survives and E.coli dies.<br><br><hr><br />
<h1><b>Gameboard Rules</b></h1><br><br />
<br />
<strong>Antibiotic Boxes</strong><br><br />
<br />
All players except Virus players are killed and returns to the last checkpoint box unless the basic gene cards or gene synthesis cards of the player have no resistance to that antibiotic. Note that there are four kinds of antibiotic boxes: Ampicilin, chloramphenicol, kanamycin and tetracyclin.<br><br><br />
<br />
<strong>Checkpoints</strong><br><br />
<br />
When player passes a checkpoint box, next time that player is killed by an effect; player resumes the game from these boxes.<br><br><br />
<br />
<strong>Restriction Enzymes</strong><br><br />
<br />
If one of the restriction sites that are denoted on the basic gene cardsmatches with the restriction enzyme, that card is put end of the card deck. If the restriction site of the character card matches, the player resumes the game from the last checkpoint box. Note that there are four restriction enzymes: EcoR1, Pst1, Spe1 and Xba1.<br><br><br />
<hr><br />
<h1><b>Cards</b></h1><br />
<br />
<h2 style="text-decoration: underline;">Character Cards</h2><br />
<br />
Each player will have a character card that gives the player some advantages and disadvantages depend on the type of the card and the situation. There are two types of character cards: Escherichia coli, Bacillus subtilis.<br><br />
<br />
On the character cards, there are two boxes for special circumstances: Genome restriction sites and super power.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Restriction site box also remains on the character cards. If the character card matches with the enzyme, player returns to last checkpoint box.<br><br><br />
<br />
<strong>Super Power</strong><br><br />
<br />
Each kind of character card has its own super power that gives them some advantages in the game.<br><br />
<br />
Bacillus Subtilis has the power of producing endospores. Thus, it is resistant to some sterilization techniques, in other word, some boxes on the board do not effect. These boxes are marked with sheriff star.<br><br />
<br />
The genome of Escherichia coli is known very well; thus E.coli has an advantage about getting gene cards easily. When an E.coli player stops on any checkpoint square (start box is not included), player draws one additional basic gene card. (Note that when player is killed and returns to last checkpoint, same rule exists.)<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gene Cards</h2><br />
<br />
There are two kinds of gene cards: Basic gene and gene synthesis cards. Only difference between these two kinds is that basic gene cards have restriction sites additionally.<br><br><br />
<br />
<strong>Anti-LPS Biofilm</strong><br><br />
<br />
This card allows B.subtilis players to kill E.coli – which means E.coli player must continue the game from the last checkpoint box – in the case of meeting on any box.<br><br><br />
<br />
<strong>Chemotaxis</strong><br><br />
<br />
This card allows playersto go forward two more boxes on the “Glucose Rich Medium” box. In contrast, on the “Hydrogen peroxide” box, players go two steps backward.<br><br><br />
<br />
<strong>Deep Freeze</strong><br><br />
<br />
This card allows the player not to wait on the “-80 C<sup>o</sup>media” box for one turn.<br><br><br />
<br />
<strong>Encapsulation</strong><br><br />
<br />
This card allows E.coli player to be treated like B.subtilis player for that turn. In another word, E.coli character card becomes B.subtilis character card for the turn that this card is used.<br><br><br />
<br />
<strong>Fenton Reagent</strong><br><br />
<br />
The player can put this card on any box on the board. Any player stops on that box, returns to last checkpoint box.<br><br><br />
<br />
<strong>iGEM</strong><br><br />
<br />
Player looks up for any gene synthesis card from its deck and exchange it with this card. (iGEM card goes under the basic gene card deck.) Gene synthesis card deck is then shuffled. This card can only be used at the beginning of a turn.<br><br><br />
<br />
<strong>Joker</strong><br><br />
<br />
This card can be treated as any kind of basic gene card. Note that this card also has all restriction sites and all kinds of antibiotic resistance.<br><br><br />
<br />
<strong>Lightening</strong><br><br />
<br />
This card allows the player not to be affected on the “Ampicilin antibiotic” box.<br><br><br />
<br />
<strong>Lysogenic Cycle</strong><br><br />
<br />
Player with this card is not affected by “Virus” box. Instead of it, player receives a “Lysogenic Cycle” marker and continues the game. If the player with that marker stops on “Virus Environment?” square, that player returns to last checkpoint. (Note that after being killed, “Lysogenic Cycle” marker is lost.)<br><br><br />
<br />
<strong>Second Chance</strong><br><br />
<br />
If a player is killed, that player resumes the game without returning to last checkpoint via using this card.<br><br><br />
<br />
<strong>Take a Photo</strong><br><br />
<br />
This card allows the player who has reached Canvas Town square to go on two more squares.<br><br />
<br />
On the gene cards there are three boxes for special circumstances: Promoter, restriction site and antibiotic resistance.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Genes have their own cleavage zones. These zones are called “restriction sites”. On the card, restriction site box shows which restriction enzyme is able to cut the gene and make it unable to be used. There are four enzymes that have their own boxes on the game board: EcoR1, Spe1, Pst1 and Xba1. Some cards may have restriction enzymes more than one.<br><br />
<br />
When player stops on a restriction site box on the board; restriction site boxes on the cards must be checked if any of them fits. The card matches with the restriction enzyme goes end of the card deck.<br><br />
<br />
Note that gene synthesis cards do not have restriction site box.<br><br><br />
<br />
<strong>Antibiotic Resistance</strong><br><br />
<br />
This box shows that the player who owns it cannot be harmed by that type of antibiotic. There are four antibiotic boxes on the game board: Chloramphenicol, ampicilin, kanamycin and tetracyclin.<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/6/60/Oyun2.png" /><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/game
Team:Fatih Turkey/game
2011-10-29T02:29:24Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:20px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<div style="float:left;margin-right:20px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><h2>Game Rules</h2></a></div><br />
<br />
<p><center><strong>GAME INTRODUCTION</strong></center><br /><br />
<br />
Lab studies are enjoyable and we are having great fun while performing the experiments. But, reaching out more people and telling about synthetic biology and genetics are very important roles considering the possible contributions of these disciplines to humanity. The earlier you reach someone, the more you help to the development of synthetic biology. <br /><br />
In order to teach the main disciplines and content of this brand new science to especially children and teenagers, we designed a Monopoly-like board game. The game is based on teaching the general logic of Biobricks and iGEM, major lab devices, major chemicals, bacteria and biological materials used in lab and our project. <br /><br />
Each player represents a kind of bacteria: B. subtilis as gram positive and E.coli as gram negative. Depending on the differences between these two kinds; players have advantages and disadvantages throughout the game. These will help the players to finish the game line first. There are also some Biobrick cards that players use during the game. These are also vital to survive in the board game unless you stop on a game square that represents deathly circumstances for bacteria. This directs the player to think like a scientist that sometimes must decide fast and effective with few facilities.<br /><br />
We recommend our game to school age children, teenagers and university students. If you want to learn and have fun while doing that, The Canvas Town Game is the thing you are looking for…</p><br />
<p>&nbsp;</p><br />
<br><br />
<p><strong><img src="https://static.igem.org/mediawiki/2011/5/50/2011-10-25_15.35.50.jpg" height="250"/></strong><br />
<strong><img src="https://static.igem.org/mediawiki/2011/6/62/DSC05608.JPG" height="250"/></strong><br />
<strong><img src="https://static.igem.org/mediawiki/2011/0/04/2011-10-26_12.58.55.jpg" height="250"/></strong></p><br />
<br />
<br><br />
<p><span lang="EN-US" xml:lang="EN-US">We played the game in our own university, and also in high schools where we went to tell about iGEM. We had a great time while playing the game with our friends and teenagers from the high schools. We also got really good reactions from the players, and one of the most interesting suggestions was that we had to take a patent for the game.</span></p><br />
<p><span lang="EN-US" xml:lang="EN-US"> Moreover, the players were taken a survey prepared to measure effect of the game. What is synthetic biology? What can be done by it? What is the logic behind the projects of iGEM? Those were the questions that they could find the answers in this game. The game not only gave them an idea about the iGEM or syhthetic biology, but it also taught them names of some mostly used antibiotics, laboratory instruments, such as autoclave, and principles of some experiments.</span></p><br />
<p><span lang="EN-US" xml:lang="EN-US">All the process is full of unforgettable moments, and they will remain in our memories for long and long years.</span></p><br />
<br /><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/game
Team:Fatih Turkey/game
2011-10-29T02:28:34Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:20px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<div style="float:left;margin-right:20px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><h2>Game Rules</h2></a></div><br />
<br />
<p><center><strong>GAME INTRODUCTION</strong></center><br /><br />
<br />
Lab studies are enjoyable and we are having great fun while performing the experiments. But, reaching out more people and telling about synthetic biology and genetics are very important roles considering the possible contributions of these disciplines to humanity. The earlier you reach someone, the more you help to the development of synthetic biology. <br /><br />
In order to teach the main disciplines and content of this brand new science to especially children and teenagers, we designed a Monopoly-like board game. The game is based on teaching the general logic of Biobricks and iGEM, major lab devices, major chemicals, bacteria and biological materials used in lab and our project. <br /><br />
Each player represents a kind of bacteria: B. subtilis as gram positive and E.coli as gram negative. Depending on the differences between these two kinds; players have advantages and disadvantages throughout the game. These will help the players to finish the game line first. There are also some Biobrick cards that players use during the game. These are also vital to survive in the board game unless you stop on a game square that represents deathly circumstances for bacteria. This directs the player to think like a scientist that sometimes must decide fast and effective with few facilities.<br /><br />
We recommend our game to school age children, teenagers and university students. If you want to learn and have fun while doing that, The Canvas Town Game is the thing you are looking for…</p><br />
<p>&nbsp;</p><br />
<br><br />
<p><strong><img src="https://static.igem.org/mediawiki/2011/5/50/2011-10-25_15.35.50.jpg" height="230"/></strong><br />
<strong><img src="https://static.igem.org/mediawiki/2011/6/62/DSC05608.JPG" height="230"/></strong><br />
<strong><img src="https://static.igem.org/mediawiki/2011/0/04/2011-10-26_12.58.55.jpg" height="230"/></strong></p><br />
<br />
<br><br />
<p><span lang="EN-US" xml:lang="EN-US">We played the game in our own university, and also in high schools where we went to tell about iGEM. We had a great time while playing the game with our friends and teenagers from the high schools. We also got really good reactions from the players, and one of the most interesting suggestions was that we had to take a patent for the game.</span></p><br />
<p><span lang="EN-US" xml:lang="EN-US"> Moreover, the players were taken a survey prepared to measure effect of the game. What is synthetic biology? What can be done by it? What is the logic behind the projects of iGEM? Those were the questions that they could find the answers in this game. The game not only gave them an idea about the iGEM or syhthetic biology, but it also taught them names of some mostly used antibiotics, laboratory instruments, such as autoclave, and principles of some experiments.</span></p><br />
<p><span lang="EN-US" xml:lang="EN-US">All the process is full of unforgettable moments, and they will remain in our memories for long and long years.</span></p><br />
<br /><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Human_Practice
Team:Fatih Turkey/Human Practice
2011-10-29T02:20:49Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
table{<br />
background:transparent;}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
h2 a, h2 a:visited {<br />
color: #333;<br />
} <br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"><br />
<table><br />
<br />
<br />
<tr style="height: 160px;"><br />
<td width="200"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide"><img src="https://static.igem.org/mediawiki/2011/2/24/Sporocideicon.jpg" width="160" /></a><br />
</td><br />
<td width="600" style="vertical-align:top;"><br />
<table><br />
<tr><br />
<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></h2></td><br />
</tr><br />
<tr><td>To remove infections B.subtilis spores from our Anti-LPS factor containing biofilm paste, fenton reagent is used before use against E.coli.</td></tr><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 160px;"><br />
<td width="200"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77"><img src="https://static.igem.org/mediawiki/2011/7/75/Secilanaokuluicon.jpg" width="160" /></a><br />
</td><br />
<td width="600" style="vertical-align:top;"><br />
<table><br />
<tr><br />
<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></h2></td><br />
</tr><br />
<tr><td>During funny summer in our project executions, we have also met hundreds of people from different ages in order to tell our project in iGEM competition and benefits of synthetic biology.</td></tr><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<tr style="height: 160px;"><br />
<td width="200"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" width="160" /></a><br />
</td><br />
<td width="600" style="vertical-align:top;"><br />
<table><br />
<tr><br />
<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></h2></td><br />
</tr><br />
<tr><td>We have designed monopoly like game name "The Canvas Town" to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick of iGEM competition.</td></tr><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<br />
<tr style="height: 160px;"><br />
<td width="200"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times"><img src="https://static.igem.org/mediawiki/2011/0/05/Canvasicon.jpg" width="160" /></a><br />
</td><br />
<td width="600" style="vertical-align:top;"><br />
<table><br />
<tr><br />
<td><h2><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></h2></td><br />
</tr><br />
<tr><td>a new journal of young scientists; it gives you the actual news of our lab and team</td></tr><br />
</td><br />
</tr><br />
</table><br />
</td><br />
</tr><br />
<br />
<br />
<br />
</table> <br />
<br />
<div align=`center`><br />
<br />
<h1>Outline and Detailed Human Practice</h1></div><br />
<br />
<div> <br />
<tr><td>During our Human Practice studies, we have tried to tell our project, its usage areas, synthetic better life not only to our medical student friends coming from different schools through Turkey but also citizens, childrens, highschool students <strong>with the help of `Canvas Town` our novel board game, `iGEM for 7 to 77` our weekly open-lab to the public meetings and our `Canvas Times` great summer magazine containing all our human practice activities. </strong><br />
<br />
<div>To be able to reach the best human practice prize;</tr></td></div> <br />
<br />
<div> <br />
<tr><td>1) Firstly, we aimed in our project, obtain an anti gram negative surface with using B. subtilis and E.coli bacteria. E. coli is killing by B. subtilis with B. subtilis’s own new protein LALF. But B. subtilis is a gram positive and this surface can’t protect us from some pathogenic effects of B.Subtilis’s spores. For protecting from B. subtilis we used <strong>the sporocide method as a non-used B.subtilis procedure in iGEM</strong> useful for further B.subtilis synthetic biology projects. Fenton Reagent in Sporocide methode is making our project more safety and useful as wanted in Human practice safety issue approaching way of our project. </tr></td></div> <br />
<br />
<div><tr><td>2) Secondly we have informed people about our project, synthetic biology and iGEM competition, and draw their attention to genetics.</td></tr></div><br />
<br />
<div><tr><td>- We prepared <strong>a board game named “The Canvas Town”</strong> to inform people from any distinct education level about features of our project, laboratory equipments and interesting Biobrick parts of iGEM competıtion. We have played several times with lots of people some of them are a group of gifted children and the Turkey’s other iGEM teams and it was very funny and informative. </tr></td></div><br />
<br />
<div><tr><td>-Our one of the most important goals is to introduce iGEM and Synthetic Biology to the students in Turkey. But we did not want to limit ourselves by choosing only same age groups and we asked to several ages of people <strong>“what do you want from your bacteria to do for you?” </strong>With this question we went to a kindergarten and we gave very interesting answers. The children were very interested about genetics, synthetic biology and microorganisms. Also we asked the same question to students of our university and the ideas were very funny and useful for synthetic biology. With these activities we drew their attention to genetics, synthetic biology and iGEM. </td></tr></div><br />
<br />
<div><tr><td>-<strong>Second International Medical Student Conference in Ankara</strong> had performed on April 2011 and it’s organized by University of Fatih Medical Faculty. In the conference, synthetic biology and iGEM competition related presentation was performed to inform medical school students coming from different medical schools in Turkey and also from out of Turkey ie. Japan.</td></tr></div><br />
<br />
<div><tr><td>3) Thirdly we have showed people that the benefits of synthetic biology and microorganisms </div><br />
<br />
<div>-We strove for the innovation and presentation of synthetic biology to people. As a medical school, our hospital has its <strong>own quarterly magazine, “Yasama Sanati” (in English: The Art of Living)</strong>. We decided that we could mention about iGEM and synthetic biology; thus we wrote an article that told the bacteria is not too much harmful living creature to humans as it is seen nowadays. In fact, we mentioned that bacteria would become very handy, helpful and intelligent “machines” in appropriate conditions. We listed the former experiments and parts that took place in iGEM competition and gave some examples about what to do next. With this article lots of people informed and their some negative sentiments about the microorganisms and synthetic biology would be changed.</td></tr></div><br />
<br />
<div><tr><td>4) We have prepared <strong>a journal which is named “Canvas Times” </strong>and it gives you the actual news of our lab and team. Also you can learn about our project and read our columnist and you can travel our peace and quiet town the Canvas Town. “Canvas Times” a new journal of young scientists; it gives you the actual news of our lab and team. <br />
During our lab working, we could find some time to fun and talk with people about our project, synthetic biology and iGEM... Our all of activities and human practices are existed on our journal such as<strong> “iGEM Picnic” and “International Medical Students Conference</strong>. Therewithal you will find some information of our university and faculty. You can find it all of stores.</div><br />
</td></tr></div><br />
<br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/game
Team:Fatih Turkey/game
2011-10-29T02:19:39Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:20px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<div style="float:left;margin-right:20px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><h2>Game Rules</h2></a></div><br />
<br />
<p><center><strong>GAME INTRODUCTION</strong></center><br /><br />
<br />
Lab studies are enjoyable and we are having great fun while performing the experiments. But, reaching out more people and telling about synthetic biology and genetics are very important roles considering the possible contributions of these disciplines to humanity. The earlier you reach someone, the more you help to the development of synthetic biology. <br /><br />
In order to teach the main disciplines and content of this brand new science to especially children and teenagers, we designed a Monopoly-like board game. The game is based on teaching the general logic of Biobricks and iGEM, major lab devices, major chemicals, bacteria and biological materials used in lab and our project. <br /><br />
Each player represents a kind of bacteria: B. subtilis as gram positive and E.coli as gram negative. Depending on the differences between these two kinds; players have advantages and disadvantages throughout the game. These will help the players to finish the game line first. There are also some Biobrick cards that players use during the game. These are also vital to survive in the board game unless you stop on a game square that represents deathly circumstances for bacteria. This directs the player to think like a scientist that sometimes must decide fast and effective with few facilities.<br /><br />
We recommend our game to school age children, teenagers and university students. If you want to learn and have fun while doing that, The Canvas Town Game is the thing you are looking for…</p><br />
<p>&nbsp;</p><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/game
Team:Fatih Turkey/game
2011-10-29T02:19:00Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:20px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<div style="float:left;margin-right:20px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><h2>Game Rules</h2></a></div><br />
<br />
<p><center>GAME INTRODUCTION</center><br /><br />
<br /><br />
Lab studies are enjoyable and we are having great fun while performing the experiments. But, reaching out more people and telling about synthetic biology and genetics are very important roles considering the possible contributions of these disciplines to humanity. The earlier you reach someone, the more you help to the development of synthetic biology. <br /><br />
In order to teach the main disciplines and content of this brand new science to especially children and teenagers, we designed a Monopoly-like board game. The game is based on teaching the general logic of Biobricks and iGEM, major lab devices, major chemicals, bacteria and biological materials used in lab and our project. <br /><br />
Each player represents a kind of bacteria: B. subtilis as gram positive and E.coli as gram negative. Depending on the differences between these two kinds; players have advantages and disadvantages throughout the game. These will help the players to finish the game line first. There are also some Biobrick cards that players use during the game. These are also vital to survive in the board game unless you stop on a game square that represents deathly circumstances for bacteria. This directs the player to think like a scientist that sometimes must decide fast and effective with few facilities.<br /><br />
We recommend our game to school age children, teenagers and university students. If you want to learn and have fun while doing that, The Canvas Town Game is the thing you are looking for…</p><br />
<p>&nbsp;</p><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/game
Team:Fatih Turkey/game
2011-10-29T02:18:23Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:20px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<div style="float:left;margin-right:20px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><h2>Game Rules</h2></a></div><br />
<br />
<p><strong>GAME INTRODUCTION</strong><br /><br />
<br /><br />
Lab studies are enjoyable and we are having great fun while performing the experiments. But, reaching out more people and telling about synthetic biology and genetics are very important roles considering the possible contributions of these disciplines to humanity. The earlier you reach someone, the more you help to the development of synthetic biology. <br /><br />
In order to teach the main disciplines and content of this brand new science to especially children and teenagers, we designed a Monopoly-like board game. The game is based on teaching the general logic of Biobricks and iGEM, major lab devices, major chemicals, bacteria and biological materials used in lab and our project. <br /><br />
Each player represents a kind of bacteria: B. subtilis as gram positive and E.coli as gram negative. Depending on the differences between these two kinds; players have advantages and disadvantages throughout the game. These will help the players to finish the game line first. There are also some Biobrick cards that players use during the game. These are also vital to survive in the board game unless you stop on a game square that represents deathly circumstances for bacteria. This directs the player to think like a scientist that sometimes must decide fast and effective with few facilities.<br /><br />
We recommend our game to school age children, teenagers and university students. If you want to learn and have fun while doing that, The Canvas Town Game is the thing you are looking for…</p><br />
<p>&nbsp;</p><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/game
Team:Fatih Turkey/game
2011-10-29T02:17:45Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<div style="float:left;margin-right:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><h2>Game Rules</h2></a></div><br />
<br />
<p><strong>GAME INTRODUCTION</strong><br /><br />
<br /><br />
Lab studies are enjoyable and we are having great fun while performing the experiments. But, reaching out more people and telling about synthetic biology and genetics are very important roles considering the possible contributions of these disciplines to humanity. The earlier you reach someone, the more you help to the development of synthetic biology. <br /><br />
In order to teach the main disciplines and content of this brand new science to especially children and teenagers, we designed a Monopoly-like board game. The game is based on teaching the general logic of Biobricks and iGEM, major lab devices, major chemicals, bacteria and biological materials used in lab and our project. <br /><br />
Each player represents a kind of bacteria: B. subtilis as gram positive and E.coli as gram negative. Depending on the differences between these two kinds; players have advantages and disadvantages throughout the game. These will help the players to finish the game line first. There are also some Biobrick cards that players use during the game. These are also vital to survive in the board game unless you stop on a game square that represents deathly circumstances for bacteria. This directs the player to think like a scientist that sometimes must decide fast and effective with few facilities.<br /><br />
We recommend our game to school age children, teenagers and university students. If you want to learn and have fun while doing that, The Canvas Town Game is the thing you are looking for…</p><br />
<p>&nbsp;</p><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/game
Team:Fatih Turkey/game
2011-10-29T02:17:19Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<div style="float:left;margin-right:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><h2>Game Rules</h2></a></div><br />
<img src="https://static.igem.org/mediawiki/2011/a/ad/Oyun1.png" /><hr><br />
<br />
<br />
<p><strong>GAME INTRODUCTION</strong><br /><br />
<br /><br />
Lab studies are enjoyable and we are having great fun while performing the experiments. But, reaching out more people and telling about synthetic biology and genetics are very important roles considering the possible contributions of these disciplines to humanity. The earlier you reach someone, the more you help to the development of synthetic biology. <br /><br />
In order to teach the main disciplines and content of this brand new science to especially children and teenagers, we designed a Monopoly-like board game. The game is based on teaching the general logic of Biobricks and iGEM, major lab devices, major chemicals, bacteria and biological materials used in lab and our project. <br /><br />
Each player represents a kind of bacteria: B. subtilis as gram positive and E.coli as gram negative. Depending on the differences between these two kinds; players have advantages and disadvantages throughout the game. These will help the players to finish the game line first. There are also some Biobrick cards that players use during the game. These are also vital to survive in the board game unless you stop on a game square that represents deathly circumstances for bacteria. This directs the player to think like a scientist that sometimes must decide fast and effective with few facilities.<br /><br />
We recommend our game to school age children, teenagers and university students. If you want to learn and have fun while doing that, The Canvas Town Game is the thing you are looking for…</p><br />
<p>&nbsp;</p><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Game_Rules
Team:Fatih Turkey/Game Rules
2011-10-29T02:15:01Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<div style="float:left;margin-right:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><h2>Game Rules</h2></a></div><br />
<img src="https://static.igem.org/mediawiki/2011/a/ad/Oyun1.png" /><hr><br />
<br />
<h1><b>Game Properties</b></h1><br />
<h2 style="text-decoration: underline">Materials</h2><br />
<ul><br />
<li>One dice</li><br />
<li>Three Bacillus Subtilis, three Escherichia Coli pawns</li><br />
<li>9 character cards, 96 basic gene cards and 96 gene synthesis cards</li><br />
<li>Gameboard</li><br />
<li>“Lysogenic Cycle” and turn standby icons</li><br />
</ul><br />
<br><br />
<h2 style="text-decoration: underline">Game Plan</h2><br />
<ul><br />
<li>At the beginning, character cards are distributed to all players randomly. Players receive their pawns that match with their character cards.</li><br />
<li>The players with same character assemble teams of kinds.</li><br />
<li>One member of each team rolls dice to determine which team is going to start first. Then, members of the first team also roll dice. Team member that has the biggest number on the dice is the first player.</li><br />
<li>Game continues counterclockwise as from the first player.</li><br />
<li>Each player goes forward times of the number on the dice.</li><br />
<li>The first player that reaches the last golden box is the winner.</li><br />
</ul><br />
<hr><br />
<h1><b>General Rules</b></h1><br />
<br />
<h2 style="text-decoration: underline">Survival</h2><br />
<br />
In the case that player can survive on a box only by using a gene card, that player must use his/her card. Use of cards that are not “vital” is optional.<br><br><br />
<br />
<h2 style="text-decoration: underline">Returning to Checkpoint</h2><br />
<br />
Bacteria can be killed by some effects. In the game, any player that is “killed” must resume the game from last checkpoint box. There are two checkpoint box and a start box. Players that could not reach the first checkpoint must return to start box if necessary.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Conjugation</h2><br />
<br />
If two members of a team meet on the same box, these players can exchange their basic gene cards.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gang-o-Bang</h2><br />
<br />
If B. Subtilis and E.coli members meet in the same box, E. coli kills B. Subtilis player. Then, B. Subtilis goes back to the last checkpoint or start point. But; if B. Subtilis has the “Anti-LPS card”, B. Subtilis survives and E.coli dies.<br><br><hr><br />
<h1><b>Gameboard Rules</b></h1><br><br />
<br />
<strong>Antibiotic Boxes</strong><br><br />
<br />
All players except Virus players are killed and returns to the last checkpoint box unless the basic gene cards or gene synthesis cards of the player have no resistance to that antibiotic. Note that there are four kinds of antibiotic boxes: Ampicilin, chloramphenicol, kanamycin and tetracyclin.<br><br><br />
<br />
<strong>Checkpoints</strong><br><br />
<br />
When player passes a checkpoint box, next time that player is killed by an effect; player resumes the game from these boxes.<br><br><br />
<br />
<strong>Restriction Enzymes</strong><br><br />
<br />
If one of the restriction sites that are denoted on the basic gene cardsmatches with the restriction enzyme, that card is put end of the card deck. If the restriction site of the character card matches, the player resumes the game from the last checkpoint box. Note that there are four restriction enzymes: EcoR1, Pst1, Spe1 and Xba1.<br><br><br />
<hr><br />
<h1><b>Cards</b></h1><br />
<br />
<h2 style="text-decoration: underline;">Character Cards</h2><br />
<br />
Each player will have a character card that gives the player some advantages and disadvantages depend on the type of the card and the situation. There are two types of character cards: Escherichia coli, Bacillus subtilis.<br><br />
<br />
On the character cards, there are two boxes for special circumstances: Genome restriction sites and super power.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Restriction site box also remains on the character cards. If the character card matches with the enzyme, player returns to last checkpoint box.<br><br><br />
<br />
<strong>Super Power</strong><br><br />
<br />
Each kind of character card has its own super power that gives them some advantages in the game.<br><br />
<br />
Bacillus Subtilis has the power of producing endospores. Thus, it is resistant to some sterilization techniques, in other word, some boxes on the board do not effect. These boxes are marked with sheriff star.<br><br />
<br />
The genome of Escherichia coli is known very well; thus E.coli has an advantage about getting gene cards easily. When an E.coli player stops on any checkpoint square (start box is not included), player draws one additional basic gene card. (Note that when player is killed and returns to last checkpoint, same rule exists.)<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gene Cards</h2><br />
<br />
There are two kinds of gene cards: Basic gene and gene synthesis cards. Only difference between these two kinds is that basic gene cards have restriction sites additionally.<br><br><br />
<br />
<strong>Anti-LPS Biofilm</strong><br><br />
<br />
This card allows B.subtilis players to kill E.coli – which means E.coli player must continue the game from the last checkpoint box – in the case of meeting on any box.<br><br><br />
<br />
<strong>Chemotaxis</strong><br><br />
<br />
This card allows playersto go forward two more boxes on the “Glucose Rich Medium” box. In contrast, on the “Hydrogen peroxide” box, players go two steps backward.<br><br><br />
<br />
<strong>Deep Freeze</strong><br><br />
<br />
This card allows the player not to wait on the “-80 C<sup>o</sup>media” box for one turn.<br><br><br />
<br />
<strong>Encapsulation</strong><br><br />
<br />
This card allows E.coli player to be treated like B.subtilis player for that turn. In another word, E.coli character card becomes B.subtilis character card for the turn that this card is used.<br><br><br />
<br />
<strong>Fenton Reagent</strong><br><br />
<br />
The player can put this card on any box on the board. Any player stops on that box, returns to last checkpoint box.<br><br><br />
<br />
<strong>iGEM</strong><br><br />
<br />
Player looks up for any gene synthesis card from its deck and exchange it with this card. (iGEM card goes under the basic gene card deck.) Gene synthesis card deck is then shuffled. This card can only be used at the beginning of a turn.<br><br><br />
<br />
<strong>Joker</strong><br><br />
<br />
This card can be treated as any kind of basic gene card. Note that this card also has all restriction sites and all kinds of antibiotic resistance.<br><br><br />
<br />
<strong>Lightening</strong><br><br />
<br />
This card allows the player not to be affected on the “Ampicilin antibiotic” box.<br><br><br />
<br />
<strong>Lysogenic Cycle</strong><br><br />
<br />
Player with this card is not affected by “Virus” box. Instead of it, player receives a “Lysogenic Cycle” marker and continues the game. If the player with that marker stops on “Virus Environment?” square, that player returns to last checkpoint. (Note that after being killed, “Lysogenic Cycle” marker is lost.)<br><br><br />
<br />
<strong>Second Chance</strong><br><br />
<br />
If a player is killed, that player resumes the game without returning to last checkpoint via using this card.<br><br><br />
<br />
<strong>Take a Photo</strong><br><br />
<br />
This card allows the player who has reached Canvas Town square to go on two more squares.<br><br />
<br />
On the gene cards there are three boxes for special circumstances: Promoter, restriction site and antibiotic resistance.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Genes have their own cleavage zones. These zones are called “restriction sites”. On the card, restriction site box shows which restriction enzyme is able to cut the gene and make it unable to be used. There are four enzymes that have their own boxes on the game board: EcoR1, Spe1, Pst1 and Xba1. Some cards may have restriction enzymes more than one.<br><br />
<br />
When player stops on a restriction site box on the board; restriction site boxes on the cards must be checked if any of them fits. The card matches with the restriction enzyme goes end of the card deck.<br><br />
<br />
Note that gene synthesis cards do not have restriction site box.<br><br><br />
<br />
<strong>Antibiotic Resistance</strong><br><br />
<br />
This box shows that the player who owns it cannot be harmed by that type of antibiotic. There are four antibiotic boxes on the game board: Chloramphenicol, ampicilin, kanamycin and tetracyclin.<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/6/60/Oyun2.png" /><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/game
Team:Fatih Turkey/game
2011-10-29T02:12:21Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Canvas Town The Game</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full" id="toptop"><br />
<div style="float:right;margin-left:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Playing_Game"><h2>Game Photos</h2></a></div><br />
<div style="float:left;margin-right:200px;"><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><img src="https://static.igem.org/mediawiki/2011/1/1b/Oyunicon.jpg" style="float:left;"/></a><a href="https://2011.igem.org/Team:Fatih_Turkey/Game_Rules"><h2>Game Rules</h2></a></div><br />
<img src="https://static.igem.org/mediawiki/2011/a/ad/Oyun1.png" /><hr><br />
<br />
<h1><b>Game Properties</b></h1><br />
<h2 style="text-decoration: underline">Materials</h2><br />
<ul><br />
<li>One dice</li><br />
<li>Three Bacillus Subtilis, three Escherichia Coli pawns</li><br />
<li>9 character cards, 96 basic gene cards and 96 gene synthesis cards</li><br />
<li>Gameboard</li><br />
<li>“Lysogenic Cycle” and turn standby icons</li><br />
</ul><br />
<br><br />
<h2 style="text-decoration: underline">Game Plan</h2><br />
<ul><br />
<li>At the beginning, character cards are distributed to all players randomly. Players receive their pawns that match with their character cards.</li><br />
<li>The players with same character assemble teams of kinds.</li><br />
<li>One member of each team rolls dice to determine which team is going to start first. Then, members of the first team also roll dice. Team member that has the biggest number on the dice is the first player.</li><br />
<li>Game continues counterclockwise as from the first player.</li><br />
<li>Each player goes forward times of the number on the dice.</li><br />
<li>The first player that reaches the last golden box is the winner.</li><br />
</ul><br />
<hr><br />
<h1><b>General Rules</b></h1><br />
<br />
<h2 style="text-decoration: underline">Survival</h2><br />
<br />
In the case that player can survive on a box only by using a gene card, that player must use his/her card. Use of cards that are not “vital” is optional.<br><br><br />
<br />
<h2 style="text-decoration: underline">Returning to Checkpoint</h2><br />
<br />
Bacteria can be killed by some effects. In the game, any player that is “killed” must resume the game from last checkpoint box. There are two checkpoint box and a start box. Players that could not reach the first checkpoint must return to start box if necessary.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Conjugation</h2><br />
<br />
If two members of a team meet on the same box, these players can exchange their basic gene cards.<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gang-o-Bang</h2><br />
<br />
If B. Subtilis and E.coli members meet in the same box, E. coli kills B. Subtilis player. Then, B. Subtilis goes back to the last checkpoint or start point. But; if B. Subtilis has the “Anti-LPS card”, B. Subtilis survives and E.coli dies.<br><br><hr><br />
<h1><b>Gameboard Rules</b></h1><br><br />
<br />
<strong>Antibiotic Boxes</strong><br><br />
<br />
All players except Virus players are killed and returns to the last checkpoint box unless the basic gene cards or gene synthesis cards of the player have no resistance to that antibiotic. Note that there are four kinds of antibiotic boxes: Ampicilin, chloramphenicol, kanamycin and tetracyclin.<br><br><br />
<br />
<strong>Checkpoints</strong><br><br />
<br />
When player passes a checkpoint box, next time that player is killed by an effect; player resumes the game from these boxes.<br><br><br />
<br />
<strong>Restriction Enzymes</strong><br><br />
<br />
If one of the restriction sites that are denoted on the basic gene cardsmatches with the restriction enzyme, that card is put end of the card deck. If the restriction site of the character card matches, the player resumes the game from the last checkpoint box. Note that there are four restriction enzymes: EcoR1, Pst1, Spe1 and Xba1.<br><br><br />
<hr><br />
<h1><b>Cards</b></h1><br />
<br />
<h2 style="text-decoration: underline;">Character Cards</h2><br />
<br />
Each player will have a character card that gives the player some advantages and disadvantages depend on the type of the card and the situation. There are two types of character cards: Escherichia coli, Bacillus subtilis.<br><br />
<br />
On the character cards, there are two boxes for special circumstances: Genome restriction sites and super power.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Restriction site box also remains on the character cards. If the character card matches with the enzyme, player returns to last checkpoint box.<br><br><br />
<br />
<strong>Super Power</strong><br><br />
<br />
Each kind of character card has its own super power that gives them some advantages in the game.<br><br />
<br />
Bacillus Subtilis has the power of producing endospores. Thus, it is resistant to some sterilization techniques, in other word, some boxes on the board do not effect. These boxes are marked with sheriff star.<br><br />
<br />
The genome of Escherichia coli is known very well; thus E.coli has an advantage about getting gene cards easily. When an E.coli player stops on any checkpoint square (start box is not included), player draws one additional basic gene card. (Note that when player is killed and returns to last checkpoint, same rule exists.)<br><br><br />
<br />
<h2 style="text-decoration: underline;">Gene Cards</h2><br />
<br />
There are two kinds of gene cards: Basic gene and gene synthesis cards. Only difference between these two kinds is that basic gene cards have restriction sites additionally.<br><br><br />
<br />
<strong>Anti-LPS Biofilm</strong><br><br />
<br />
This card allows B.subtilis players to kill E.coli – which means E.coli player must continue the game from the last checkpoint box – in the case of meeting on any box.<br><br><br />
<br />
<strong>Chemotaxis</strong><br><br />
<br />
This card allows playersto go forward two more boxes on the “Glucose Rich Medium” box. In contrast, on the “Hydrogen peroxide” box, players go two steps backward.<br><br><br />
<br />
<strong>Deep Freeze</strong><br><br />
<br />
This card allows the player not to wait on the “-80 C<sup>o</sup>media” box for one turn.<br><br><br />
<br />
<strong>Encapsulation</strong><br><br />
<br />
This card allows E.coli player to be treated like B.subtilis player for that turn. In another word, E.coli character card becomes B.subtilis character card for the turn that this card is used.<br><br><br />
<br />
<strong>Fenton Reagent</strong><br><br />
<br />
The player can put this card on any box on the board. Any player stops on that box, returns to last checkpoint box.<br><br><br />
<br />
<strong>iGEM</strong><br><br />
<br />
Player looks up for any gene synthesis card from its deck and exchange it with this card. (iGEM card goes under the basic gene card deck.) Gene synthesis card deck is then shuffled. This card can only be used at the beginning of a turn.<br><br><br />
<br />
<strong>Joker</strong><br><br />
<br />
This card can be treated as any kind of basic gene card. Note that this card also has all restriction sites and all kinds of antibiotic resistance.<br><br><br />
<br />
<strong>Lightening</strong><br><br />
<br />
This card allows the player not to be affected on the “Ampicilin antibiotic” box.<br><br><br />
<br />
<strong>Lysogenic Cycle</strong><br><br />
<br />
Player with this card is not affected by “Virus” box. Instead of it, player receives a “Lysogenic Cycle” marker and continues the game. If the player with that marker stops on “Virus Environment?” square, that player returns to last checkpoint. (Note that after being killed, “Lysogenic Cycle” marker is lost.)<br><br><br />
<br />
<strong>Second Chance</strong><br><br />
<br />
If a player is killed, that player resumes the game without returning to last checkpoint via using this card.<br><br><br />
<br />
<strong>Take a Photo</strong><br><br />
<br />
This card allows the player who has reached Canvas Town square to go on two more squares.<br><br />
<br />
On the gene cards there are three boxes for special circumstances: Promoter, restriction site and antibiotic resistance.<br><br><br />
<br />
<strong>Restriction Site</strong><br><br />
<br />
Genes have their own cleavage zones. These zones are called “restriction sites”. On the card, restriction site box shows which restriction enzyme is able to cut the gene and make it unable to be used. There are four enzymes that have their own boxes on the game board: EcoR1, Spe1, Pst1 and Xba1. Some cards may have restriction enzymes more than one.<br><br />
<br />
When player stops on a restriction site box on the board; restriction site boxes on the cards must be checked if any of them fits. The card matches with the restriction enzyme goes end of the card deck.<br><br />
<br />
Note that gene synthesis cards do not have restriction site box.<br><br><br />
<br />
<strong>Antibiotic Resistance</strong><br><br />
<br />
This box shows that the player who owns it cannot be harmed by that type of antibiotic. There are four antibiotic boxes on the game board: Chloramphenicol, ampicilin, kanamycin and tetracyclin.<hr><br />
<br />
<img src="https://static.igem.org/mediawiki/2011/6/60/Oyun2.png" /><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Sporocide
Team:Fatih Turkey/Sporocide
2011-10-29T02:00:46Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporiocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Sporicide</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<center> <b >EFFECTIVE SOLUTION TO BIG DANGER: SPORICIDE </b></center><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/0/02/DSC03586_-_Kopya.JPG" weight="300"></center></p><br />
<br />
<br><br />
<p><center><em>Left: fenton reagent + aqueous dissolved oxygen<br /><br />
Right: fenton reagent + distilled water </em></center> <br /><br />
The reason that we chose B.subtilis in our project is the LPS binding character of LALF. Gram positive bacteria such as B.subtilis have no LPS layer on their cell wall. This obligation of choosing alternative bacterium leads to another problem: B.subtilis has the ability to form endospores. <br /><br />
B.subtilis, normally, has no pathogenic character unless it forms endospores under some circumstances like high temperature, limit pH levels or deoxygenized media. The inhalation of endospores is very dangerous and must be avoided in the case of study on B.subtilis.<br /><br />
Decontamination of biological endospores is very important considering the necessary of the safety in synthetic biology laboratories. Especially in iGEM, teams may need alternative creatures to plan and perform their projects properly like in our project. Because of this, we began to look through the web and hope to find a solution for this safety problem. Fortunately, some specific dilutions are characterized to solve possible safety problems in order to assemble aseptic surfaces. <strong>Fenton reagents</strong>, which kill septic microorganisms with mechanism of hydroxyl radicals, were traditionally being used. However; its major ingredient, hydrogen peroxide, was examined and was compared with aqueous dissolved oxygen about their capability of killing the spores. (J. B. Cross, 2003)1<br />
<br /><br />
It is said that in addition to aqueous dissolved oxygen, cupric chloride, ascorbic acid, sodium chloride and a little sulfactant dilution which are used as modified Fenton reagent are more effective on endospores of gram positive bacteria. In contrast; classic Fenton reagent, which possess hydrogen peroxide and copper ion, shows that gram negative bacteria are more vulnerable to this formulation. The mechanism of these dilutions is not well-characterized; however, comparing with other dilutions that include specific oxidizing agents such as ozone, some differences in the images that are gained from effected bacteria are indicated. It is deduced that modified Fenton reagent affects the inside mechanism of the bacteria; although the dilutions with ozone damages the coat of the bacteria. After formation of hydroxyl radicals within the spore, these highly reactive molecules attack either the DNA or enzymes necessary for the spore conversion to a bacterial cell. Further investigations of ion transport into spores and oxidation-reductions with in spores will be needed to determine precisely the mechanism of spore kill under these conditions.<br /><br />
The effectiveness of modified Fenton Reagent and the advantages/disadvantages of different Fenton Reagent solutions are characterized during our experiments. For more information about Fenton Reagent experiments, please visit <u>Fenton Reagent Experiments</u> page.<br /><br />
Another problem we faced was that the sporicide can also kill gram negative bacteria in their non-endospore forms. Our LALF protein is there for this reason; but we should be sure that gram negative bacteria are terminated just because of LALF protein. To achieve this, we decided to extract the oxygen ingradient which is the main component of killing mechanism in the Fenton reagent. Thus, hydroxyl radical mechanism of their agents would not work and only mechanism on the surface as terminator would be our anti-LPS factor against the gram negative bacteria, especially E.coli in our project.<br /><br />
Considering the importance of safe and secure labs in synthetic biology, especially in iGEM, prospective projects including B.subtilis may confront such problems. The need of sporicide will increase in the next iGEM competitions. To have safer labs, we recommend our chemical decontaminator to all iGEM teams.</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p><strong>DISCUSSION</strong><br /><br />
As a conclusion, we observed <strong>distilled water</strong> included Fenton Reagent (DFR) is more effective than <strong>oxygenated water</strong> included Fenton Reagent (OFR) against E.coli. When 25 uL OFR and DFR have been added in E.coli cultures, 82% of DFR treated culture was dead and 47% of OFR treated culture was dead. We did not see any colony which included 50 uL and more volumes of DFR. In contrast, after 75 uL and more addition of OFR, no colony formation has been observed.</p><br />
<p>On the other hand, we observed OFR is more effective than DFR against Bacillus subtilis. When 10 uL OFR and DFR have been added in Bacillus subtilis cultures, 38% of DFR treated culture was dead and 98.3% of OFR treated culture was dead. Not only the 25 uL OFR treated but also 25 uL DFR treated culture was dead.</p><br />
<p>If we want to compare E.coli and B. subtilis, we can say that Fenton Reagent application is more fatal for Bacillus subtilis. Both 25 uL OFR and DFR treated Bacillus subtilis culture was dead but 25 uL OFR and DFR treated E.coli culture was still alive.</p><br />
<br />
<br><br />
<p>1 Killing of Bacillus Spores by Aqueous Dissolved Oxygen, Ascorbic Acid, and Copper Ions; J. B. Cross, R. P. Currier, D. J. Torraco, L. A. Vanderberg, G. L. Wagner, and P. D. Gladen Chemistry Division1 and Biosciences Division,2 Los Alamos National Laboratory, Los Alamos, New Mexico 87545 </p><br />
<br><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Sporocide
Team:Fatih Turkey/Sporocide
2011-10-29T02:00:01Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporiocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Sporicide</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<center> <b >EFFECTIVE SOLUTION TO BIG DANGER: SPORICIDE </b></center><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/0/02/DSC03586_-_Kopya.JPG" weight="300"></center></p><br />
<br />
<br><br />
<p><em>Left: fenton reagent + aqueous dissolved oxygen<br /><br />
Right: fenton reagent + distilled water </em> <br /><br />
The reason that we chose B.subtilis in our project is the LPS binding character of LALF. Gram positive bacteria such as B.subtilis have no LPS layer on their cell wall. This obligation of choosing alternative bacterium leads to another problem: B.subtilis has the ability to form endospores. <br /><br />
B.subtilis, normally, has no pathogenic character unless it forms endospores under some circumstances like high temperature, limit pH levels or deoxygenized media. The inhalation of endospores is very dangerous and must be avoided in the case of study on B.subtilis.<br /><br />
Decontamination of biological endospores is very important considering the necessary of the safety in synthetic biology laboratories. Especially in iGEM, teams may need alternative creatures to plan and perform their projects properly like in our project. Because of this, we began to look through the web and hope to find a solution for this safety problem. Fortunately, some specific dilutions are characterized to solve possible safety problems in order to assemble aseptic surfaces. <strong>Fenton reagents</strong>, which kill septic microorganisms with mechanism of hydroxyl radicals, were traditionally being used. However; its major ingredient, hydrogen peroxide, was examined and was compared with aqueous dissolved oxygen about their capability of killing the spores. (J. B. Cross, 2003)1<br />
<br /><br />
It is said that in addition to aqueous dissolved oxygen, cupric chloride, ascorbic acid, sodium chloride and a little sulfactant dilution which are used as modified Fenton reagent are more effective on endospores of gram positive bacteria. In contrast; classic Fenton reagent, which possess hydrogen peroxide and copper ion, shows that gram negative bacteria are more vulnerable to this formulation. The mechanism of these dilutions is not well-characterized; however, comparing with other dilutions that include specific oxidizing agents such as ozone, some differences in the images that are gained from effected bacteria are indicated. It is deduced that modified Fenton reagent affects the inside mechanism of the bacteria; although the dilutions with ozone damages the coat of the bacteria. After formation of hydroxyl radicals within the spore, these highly reactive molecules attack either the DNA or enzymes necessary for the spore conversion to a bacterial cell. Further investigations of ion transport into spores and oxidation-reductions with in spores will be needed to determine precisely the mechanism of spore kill under these conditions.<br /><br />
The effectiveness of modified Fenton Reagent and the advantages/disadvantages of different Fenton Reagent solutions are characterized during our experiments. For more information about Fenton Reagent experiments, please visit <u>Fenton Reagent Experiments</u> page.<br /><br />
Another problem we faced was that the sporicide can also kill gram negative bacteria in their non-endospore forms. Our LALF protein is there for this reason; but we should be sure that gram negative bacteria are terminated just because of LALF protein. To achieve this, we decided to extract the oxygen ingradient which is the main component of killing mechanism in the Fenton reagent. Thus, hydroxyl radical mechanism of their agents would not work and only mechanism on the surface as terminator would be our anti-LPS factor against the gram negative bacteria, especially E.coli in our project.<br /><br />
Considering the importance of safe and secure labs in synthetic biology, especially in iGEM, prospective projects including B.subtilis may confront such problems. The need of sporicide will increase in the next iGEM competitions. To have safer labs, we recommend our chemical decontaminator to all iGEM teams.</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p><strong>DISCUSSION</strong><br /><br />
As a conclusion, we observed <strong>distilled water</strong> included Fenton Reagent (DFR) is more effective than <strong>oxygenated water</strong> included Fenton Reagent (OFR) against E.coli. When 25 uL OFR and DFR have been added in E.coli cultures, 82% of DFR treated culture was dead and 47% of OFR treated culture was dead. We did not see any colony which included 50 uL and more volumes of DFR. In contrast, after 75 uL and more addition of OFR, no colony formation has been observed.</p><br />
<p>On the other hand, we observed OFR is more effective than DFR against Bacillus subtilis. When 10 uL OFR and DFR have been added in Bacillus subtilis cultures, 38% of DFR treated culture was dead and 98.3% of OFR treated culture was dead. Not only the 25 uL OFR treated but also 25 uL DFR treated culture was dead.</p><br />
<p>If we want to compare E.coli and B. subtilis, we can say that Fenton Reagent application is more fatal for Bacillus subtilis. Both 25 uL OFR and DFR treated Bacillus subtilis culture was dead but 25 uL OFR and DFR treated E.coli culture was still alive.</p><br />
<br />
<br><br />
<p>1 Killing of Bacillus Spores by Aqueous Dissolved Oxygen, Ascorbic Acid, and Copper Ions; J. B. Cross, R. P. Currier, D. J. Torraco, L. A. Vanderberg, G. L. Wagner, and P. D. Gladen Chemistry Division1 and Biosciences Division,2 Los Alamos National Laboratory, Los Alamos, New Mexico 87545 </p><br />
<br><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Experiments6
Team:Fatih Turkey/Experiments6
2011-10-29T01:39:36Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Experiments</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Existence of Reflectin</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="float: left; width: 220px; border-radius: 10px; position: absolute; top: 50px; left: 0; background: #EEE; border: solid 3px #666;"><br />
<ol><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/confirmation">Charactarization</a></li><br />
<br />
</li><br />
<br />
<br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments1">Exposure Experiment</a></li><br />
<br />
<br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiment3C">Inhibition in Liquid Culture Assay</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments4">Inhibition in Supernatant Assay</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments2">The Suicide Experiment of E.Coli</a></li> <br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments5">Fenton Reagent Assay</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments6">Existence of Reflectin</a></li><br />
<br />
</ol><br />
</div><br />
<div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;"><br />
<br />
<table width="606" height="227" border="0"><br />
<tr><br />
<td width="600" height="223" valign="top"><p><strong>Reflectin Existence Assay</strong><br /><br />
<p><strong><img src="https://static.igem.org/mediawiki/2011/9/9a/Aaa4.png" width="280" /></strong><br />
<strong><img src="https://static.igem.org/mediawiki/2011/0/0f/Aaa5.png" width="280" /></strong></p><br />
<br><br />
<p><br />
We transfered the plasmid which includes the gene part of reflectin protein (BBa_K541596) to E. Coli and we saw that there is a difference between reflectin including colonies and the control group.<br /><br />
We saw that the plate which does not include reflectin protein is normally transparent. But the plate with the reflectin synthesizing bacteria seemed white. This makes us think that our plasmid is working and the protein is produced by E. coli.<br /><br />
Additionally, the colonies are looked through light microscope. The colony which was supposed to produce reflectin had some transparent points. In contrast, the control group had completely different image. We think that reflectin protein caused these points; thus reflectin might be synthesized. However, we did not image the effect of reflectin; so, we do not know whether reflectin works as it is expected.</p></td><br />
</tr><br />
</table><br />
<br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Experiments
Team:Fatih Turkey/Experiments
2011-10-29T01:36:38Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Experiments</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Charactarization</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="float: left; width: 220px; border-radius: 10px; position: absolute; top: 50px; left: 0; background: #EEE; border: solid 3px #666;"><br />
<ol><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/confirmation">Charactarization</a></li><br />
<br />
</li><br />
<br />
<br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments1">Exposure Experiment</a></li><br />
<br />
<br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiment3C">Inhibition in Liquid Culture Assay</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments4">Inhibition in Supernatant Assay</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments2">The Suicide Experiment of E.Coli</a></li> <br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments5">Fenton Reagent Assay</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments6">Existence of Reflectin</a></li><br />
<br />
</ol><br />
</div><br />
<div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;"><br />
<table width="606" height="203" border="0"><br />
<tr><br />
<td width="600" valign="top"><p><strong>CHARACTERIZATION</strong><br /><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/5/59/Chrac.png" width="500" /></center></p><br />
<p>Before starting assays; we needed to be sure that our genes had been cloned correctly. In order to use our parts in our experiments, we confirmed our parts via electrophoresis. Following parts are fully confirmed:</p><br />
<table border="1" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td width="307" height="35" valign="top"><strong>Subparts</strong> </td><br />
<td width="307" valign="top"><p><strong>Parts</strong></p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541501</p></td><br />
<td width="307" valign="top"><p>BBa_K541515</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541502</p></td><br />
<td width="307" valign="top"><p>BBa_K541545</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541503</p></td><br />
<td width="307" valign="top"><p>BBa_K541596</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541504</p></td><br />
<td width="307" valign="top"><p>BBa_K541915</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541505</p></td><br />
<td width="307" valign="top"><p>BBa_K541800</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541506</p></td><br />
<td width="307" valign="top"><p>BBa_K541516</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>&nbsp;</p></td><br />
<td width="307" valign="top"><p>BBa_K541526</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>&nbsp;</p></td><br />
<td width="307" valign="top"><p>BBa_K541546</p></td><br />
</tr><br />
</table><br />
<p>After electrophoresis results; we furthermore performed sequencing and imaging tests to characterize our BioBricks. For BBa_541505 and BBa_541506; we added a GFP sequence and transformated into E.coli. This has characterized our main proteins. cDNA sequencing for these parts was also done successfully.<br /><br />
In addition, BBa_541800 is transformated into B.subtilis and successfully imaged red that shows RFP region and therefore plasmid backbone works properly.<br /><br />
We characterized BBa_K541545, BBa_K541515 and BBa_K541915 via sequencing.</p></td><br />
</tr><br />
</table><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/b/bc/Jelgoruntusu.jpg" width="500" /></center></p><br />
<br><br />
<br><br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Safety
Team:Fatih Turkey/Safety
2011-10-29T01:30:59Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ol, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Safety</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;"></h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"><br />
<br />
<p>FATIH UNIVERSITY MEDICAL SCHOOL ANSWERS TO NEW SAFETY AND SECURITY QUESTIONS<br /><br />
<br><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/a/a1/Donoteatordrink.jpg" width="300" /></center></p><br />
<br><br />
<br />
<strong>1. Would the materials used in your project and/or your final product pose:</strong><br /><br />
<strong>a. Risks to the safety and health of team members or others in the lab?</strong></p><br />
<p>Actually few hazardous chemicals and solutions such as EtBr are used in some lab procedures such as gel preparation and electrophoresis. However these chemicals and solutions are used according to the safety rules of the laboratory with care and caution. All the members were trained for safety regulations of the laboratory as well as toxicity of the chemicals and solutions before starting the current project.<br /><br />
<strong>b. Risks to the safety and health of the general public if released by design or accident?</strong></p><br />
<p>When released by accident, our parts and materials actually cause no negative damage to the general public. B.subtilis is known not to be associated with disease process in humans in regular conditions, and thus, is supposed to be benign by nature and present no danger to anyone. Only under stress conditions, B. subtilis may form spores and these spores are resistant versions of single cells. While B. subtilis itself is not pathogenic, its spores may be infectious if inhaled. But all of our team members are trained for safety of the laboratory rules and use mask and gloves when dealing with bacteria. Besides all experiments are performed in synthetic laboratory designated by Fatih University Medical School and no material is carried out of the laboratory. However to increase public safety of our project, in case of accident, our plan is to destroy particles and spores of B.subtilis by treating with Fenton reagent solutions containing dissolved oxygen and other constituents.<br /><br />
<strong>c. Risks to environmental quality if released by design or accident?</strong></p><br />
<p>Bacillus subtilis strain 3610 is a wild type strain that is already widespread in the environment. B. subtilis strain 168 is a derivative strain of the 3610 and has disabling auxotrophs mutations that make it even less likely to colonize or cause harm to human or animal health. Our experiment does not include any modifications, which enhance their ability to survive or disseminate. Thus, there is no specific environmental risk associated with use of the Bacillus subtilis strains except its spore formation. In case of accident, cleaning and treating the spores with Fenton reagent as mentioned above provide environmental safety. Besides, all procedures are performed in a specified laboratory.</p><br />
<p>E. Coli strains TOP10 and BL1 have very limited ability to survive outside the laboratory; so that, it would be unable to survive or disseminate. Therefore, there is no specific environmental risk associated with the E. coli strains. All bacterial wastes are kept in 10% bleaching solution for one day, and then, are autoclaved to be sterilized.<br /><br />
<strong>d. Risks to security through malicious misuse by individuals, groups or states?</strong></p><br />
<p>Please explain your responses (whether yes or no) to these questions. Specifically, are any parts or devices in your project associated with (or known to cause):</p><br />
<p><strong> * pathogenicity, infectivity, or toxicity?</strong> No<br /><br />
<strong> * threats to environmental quality?</strong> No<br /><br />
<strong> * security concerns?</strong> No</p><br />
<p>As explained above:<br /><br />
Nothing could be used maliciously in our project. The materials we use are commonly used in experimental procedures in various laboratories all over the world and all are harmless and do not pose any threat to environment and public as well as security concerns.</p><br />
<p>Throughout the project, we used the Escherichia coli strains Top10 and BL1; Bacillus subtilis strains 3610 and 168. Wild-type E. coli is classified as hazard group 2 pathogen by the UK Advisory Committee on the Dangerous Pathogens (ACDP) and Wild-type Bacillus subtilis (i.e. strain 3610) is classified as hazard group 1 organism by the ACDP and its derivative B. subtilis strain 168 has disabling auxotrophs mutations (e.g. conferring a requirement for tryptophan, Zeigler DR et al, 2008. J of Bacteriology) that makes it even less likely to colonize or cause harm to human or animal health. B.subtilis is at level 1 biosafety according to World Health Organization (WHO).<br /><br />
<strong>2. If your response to any of the questions above is yes:</strong><br /><br />
<strong>a. Explain how you addressed these issues in project design and while conducting laboratory work.</strong><br /><br />
<strong>b. Describe and document safety, security, health and/or environmental issues as you submit your parts to the Registry</strong><br /><br />
<strong>3. Under what biosafety provisions will / do you operate?</strong><br /><br />
<strong>a. Does your institution have its own biosafety rules and if so what are they? Provide a link to them online if possible</strong></p><br />
<p>Yes, our institution has its own biosafety rules. Rules for laboratory use, general principles, prevention from hazardous materials and application of emergency intervention in case of accident are included. The web link is given as (http://www.fatihmed.edu.tr/icerik/guvenlikkilavuzu.php).<br /><br />
<strong>b. Does your institution have an Institutional Biosafety Committee or equivalent group? If yes, have you discussed your project with them? Describe any concerns or changes that were made based on this review</strong></p><br />
<p>Yes. In Fatih University Medical School, Laboratory and Patient-Employee Safety Committee is responsible for control as well as biosafety of laboratories and safety of patients and employees. This committee works under one of the vice medical director of Fatih University Hospital, Prof. Dr. Mehmet Gunduz. Form on safety rules of Fatih University Medical School Laboratory use was filled in as required. We discussed our project with Prof. Dr. Mehmet Gunduz. Safety and security issues are found sufficient enough that no change is considered as necessary. (Further questions can be directed to Prof. Gunduz, tel: +90-312-203 5103, mgunduz@fatih.edu.tr)<br /><br />
<strong>c. Will / did you receive any biosafety and/or lab training before beginning your project? If so, describe this training.</strong></p><br />
<p>Yes, we did. Our advisor provided us with biosafety and lab training before starting our project. In the training, general safety rules of laboratory use, prevention from hazardous chemicals and solutions as well as emergency intervention in case of accident were included such as:</p><br />
<ul><br />
<li> - Eating, drinking, storing food and smoking are absolutely not allowed. </li><br />
<li> - Mouth pipetting is not allowed; modern pipettes are used in lab.</li><br />
<li> - Hazardous wastes and ordinary wastes are separated and cautiously disposed.</li><br />
<li> - Lab coats are obligatory to wear in the lab and during on-going experiments.</li><br />
<li> - In electrophoresis room, lab coat, protective eyewear, lab masks and gloves have to be worn. In the case of use of EtBr; extra caution is required.</li><br />
<li> - Washing hands after any experiment and after touching anything related with viable material is obliged.</li><br />
<li> - Air conditioning is kept closed during on-going experiments in order to avoid possible infections of spores and bacteria.</li><br />
</ul><br />
<p><strong>d. Does your country have national biosafety regulations or guidelines? If so, provide a link to them online if possible.</strong></p><br />
<p>Yes, Turkey has national biosafety regulations and the link is given as (http://www.tbbdm.gov.tr/Home/BioSafetyCouncilHome/BioSafetyCouncilHomeChoose.aspx).<br /><br />
<strong>4. OPTIONAL QUESTION: Do you have other ideas on how to deal with safety or security issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</strong></p><br />
<p> - First, IGEM committee may prepare a lab safety acknowledgement form like OSHA form. This form can be required to be filled in by all members. This will allow us to confirm whether members of the teams are informed about safety issues and standardize the safety measurements.<br /><br />
- Second, a new prize related with security and safety issues may take place in the judging procedure separated from “Best Human Practices” prize. Existence of “Best Safety Part” or “Best Safety Application” can emphasize the importance of the safety issues and inspire iGEM teams to work on these issues and may give a chance to develop new ideas, to interrogate current safety and security applications in order to improve them.<br /><br />
- Third, iGEM committee may organize a webinar to inform the participants about safety and security in labs. Webinar can be done at the beginning of the experiments. Mentor scientists may talk about their experiences and mention some tricks about safety issues.</p><br />
<br />
<br><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/a/a7/Goggles.jpg" height="200"/><br />
<img src="https://static.igem.org/mediawiki/2011/7/71/Lab_safety_guy.jpg" height="200"/></center></p><br />
<br />
<br><br><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Future_Plan
Team:Fatih Turkey/Future Plan
2011-10-29T01:25:03Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
ol{<br />
font-family: Verdana, Arial, SunSans-Regular, sans-serif;<br />
font-size: 12px;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Future Plan</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<p>Our project may suggest some useful ideas about varying applications that may be very crucial in the future.<br /><br />
For our Limulus anti lipopolysaccharide factor protein (LALF); some idea suggestions can be enumerated as:</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/67/Fp3.jpg" alt="Fp3.jpg (250×167)"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/68/Fp1.jpg"/> &nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/4/46/Fp5.jpg" alt="Fp5.jpg (250×188)" width="226" height="168"/> </p><br />
<ol start="1" type="1"><br />
<li>Alternative surface sterilization technique; in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization. Considering the diseases and pandemics caused by gram negatives, (including the recent EHEC pandemic occurred in Europe), this possibility underlines the importance of LALF and similar factors.</li><br />
</ol><br />
<ol start="2" type="1"><br />
<li>Alternative cure for septic shock; after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.</li><br />
<li>Possible new structure of antibiotic-like drug; antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of the production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections. In addition, in biological laboratories, LALF may offer an alternative decontamination method.</li><br />
</ol><br />
<ol start="4" type="1"><br />
<li>Possibility of producing longtime sterile layers; if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In the case of a possible infection on surface, LALF will stop bacteria growth.</li><br />
<li>Alternative cure method for bacterial diseases; diseases like urinary system infection or gastritis are caused by gram negative bacteria. In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.</li><br />
</ol><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<ol start="1" type="1"><br />
<li>Camouflage material; this protein allows its host, cephalopod, to hide when cephalopod hunts or defends itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans</li><br />
<li>New material of dye for varying applications; Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<br /><br />
</li><br />
<li> Possibility of producing specialized light colors; in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<br /><br />
</li><br />
<li> It is planned that E.coli would synthesize reflectin while B.subtilis would produce LALF. Thus, as the growth of E.coli slows down and, finally, stops; thickness of layer of E.coli colonies would thinner than the thickness of B.subtilis layer. Depending on tinting ability of reflectin, we would observe the media in different colors that indicates the result very easily. This sample presents us a new biosensor method in prospective lab experiments.</li><br />
</ol><br />
<p>&nbsp;</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2011/9/94/Fp4.jpg" width="127" height="212"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp; <img src="https://static.igem.org/mediawiki/2011/b/b3/Fp2.jpg" alt="Fp2.jpg (250×159)" width="334" height="212"/></p><br />
<p>Beyond the above, we are also planning to</p><br />
<ol><br />
<ol><br />
<li>…purify B.subtilis biofilm with LALF via Fenton Reagent solution. Fenton Reagent is supposed to kill B.subtilis colonies and endospores. Alternative methods would make this model possible.</li><br />
<li>…see tinting ability of reflectin. Reflectin can be purified and observed with specific methods.</li><br />
</ol><br />
</ol><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Future_Plan
Team:Fatih Turkey/Future Plan
2011-10-29T01:24:30Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
ol{<br />
font-family: Verdana, Arial, SunSans-Regular, sans-serif;<br />
font-size: 12px;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Future Plan</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<p>Our project may suggest some useful ideas about varying applications that may be very crucial in the future.<br /><br />
For our Limulus anti lipopolysaccharide factor protein (LALF); some idea suggestions can be enumerated as:</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/67/Fp3.jpg" alt="Fp3.jpg (250×167)"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/68/Fp1.jpg"/> &nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/4/46/Fp5.jpg" alt="Fp5.jpg (250×188)" width="226" height="168"/> </p><br />
<ol start="1" type="1"><br />
<li>Alternative surface sterilization technique; in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization. Considering the diseases and pandemics caused by gram negatives, (including the recent EHEC pandemic occurred in Europe), this possibility underlines the importance of LALF and similar factors.</li><br />
</ol><br />
<ol start="2" type="1"><br />
<li>Alternative cure for septic shock; after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.</li><br />
<li>Possible new structure of antibiotic-like drug; antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of the production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections. In addition, in biological laboratories, LALF may offer an alternative decontamination method.</li><br />
</ol><br />
<ol start="4" type="1"><br />
<li>Possibility of producing longtime sterile layers; if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In the case of a possible infection on surface, LALF will stop bacteria growth.</li><br />
<li>Alternative cure method for bacterial diseases; diseases like urinary system infection or gastritis are caused by gram negative bacteria. In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.</li><br />
</ol><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<ol start="1" type="1"><br />
<li>Camouflage material; this protein allows its host, cephalopod, to hide when cephalopod hunts or defends itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans</li><br />
<li>New material of dye for varying applications; Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<br /><br />
</li><br />
<li> Possibility of producing specialized light colors; in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<br /><br />
</li><br />
<li> It is planned that E.coli would synthesize reflectin while B.subtilis would produce LALF. Thus, as the growth of E.coli slows down and, finally, stops; thickness of layer of E.coli colonies would thinner than the thickness of B.subtilis layer. Depending on tinting ability of reflectin, we would observe the media in different colors that indicates the result very easily. This sample presents us a new biosensor method in prospective lab experiments.</li><br />
</ol><br />
<p>&nbsp;</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp&; nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<img src="https://static.igem.org/mediawiki/2011/9/94/Fp4.jpg" width="127" height="212"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp; <img src="https://static.igem.org/mediawiki/2011/b/b3/Fp2.jpg" alt="Fp2.jpg (250×159)" width="334" height="212"/></p><br />
<p>Beyond the above, we are also planning to</p><br />
<ol><br />
<ol><br />
<li>…purify B.subtilis biofilm with LALF via Fenton Reagent solution. Fenton Reagent is supposed to kill B.subtilis colonies and endospores. Alternative methods would make this model possible.</li><br />
<li>…see tinting ability of reflectin. Reflectin can be purified and observed with specific methods.</li><br />
</ol><br />
</ol><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Future_Plan
Team:Fatih Turkey/Future Plan
2011-10-29T01:23:34Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
ol{<br />
font-family: Verdana, Arial, SunSans-Regular, sans-serif;<br />
font-size: 12px;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Future Plan</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<p>Our project may suggest some useful ideas about varying applications that may be very crucial in the future.<br /><br />
For our Limulus anti lipopolysaccharide factor protein (LALF); some idea suggestions can be enumerated as:</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/67/Fp3.jpg" alt="Fp3.jpg (250×167)"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/68/Fp1.jpg"/> &nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/4/46/Fp5.jpg" alt="Fp5.jpg (250×188)" width="226" height="168"/> </p><br />
<ol start="1" type="1"><br />
<li>Alternative surface sterilization technique; in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization. Considering the diseases and pandemics caused by gram negatives, (including the recent EHEC pandemic occurred in Europe), this possibility underlines the importance of LALF and similar factors.</li><br />
</ol><br />
<ol start="2" type="1"><br />
<li>Alternative cure for septic shock; after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.</li><br />
<li>Possible new structure of antibiotic-like drug; antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of the production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections. In addition, in biological laboratories, LALF may offer an alternative decontamination method.</li><br />
</ol><br />
<ol start="4" type="1"><br />
<li>Possibility of producing longtime sterile layers; if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In the case of a possible infection on surface, LALF will stop bacteria growth.</li><br />
<li>Alternative cure method for bacterial diseases; diseases like urinary system infection or gastritis are caused by gram negative bacteria. In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.</li><br />
</ol><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<ol start="1" type="1"><br />
<li>Camouflage material; this protein allows its host, cephalopod, to hide when cephalopod hunts or defends itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans</li><br />
<li>New material of dye for varying applications; Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<br /><br />
</li><br />
<li> Possibility of producing specialized light colors; in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<br /><br />
</li><br />
<li> It is planned that E.coli would synthesize reflectin while B.subtilis would produce LALF. Thus, as the growth of E.coli slows down and, finally, stops; thickness of layer of E.coli colonies would thinner than the thickness of B.subtilis layer. Depending on tinting ability of reflectin, we would observe the media in different colors that indicates the result very easily. This sample presents us a new biosensor method in prospective lab experiments.</li><br />
</ol><br />
<p>&nbsp;</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/9/94/Fp4.jpg" width="127" height="212"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp; <img src="https://static.igem.org/mediawiki/2011/b/b3/Fp2.jpg" alt="Fp2.jpg (250×159)" width="334" height="212"/></p><br />
<p>Beyond the above, we are also planning to</p><br />
<ol><br />
<ol><br />
<li>…purify B.subtilis biofilm with LALF via Fenton Reagent solution. Fenton Reagent is supposed to kill B.subtilis colonies and endospores. Alternative methods would make this model possible.</li><br />
<li>…see tinting ability of reflectin. Reflectin can be purified and observed with specific methods.</li><br />
</ol><br />
</ol><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Future_Plan
Team:Fatih Turkey/Future Plan
2011-10-29T01:23:08Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
ol{<br />
font-family: Verdana, Arial, SunSans-Regular, sans-serif;<br />
font-size: 12px;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Future Plan</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<p>Our project may suggest some useful ideas about varying applications that may be very crucial in the future.<br /><br />
For our Limulus anti lipopolysaccharide factor protein (LALF); some idea suggestions can be enumerated as:</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/67/Fp3.jpg" alt="Fp3.jpg (250×167)"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/68/Fp1.jpg"/> &nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/4/46/Fp5.jpg" alt="Fp5.jpg (250×188)" width="226" height="168"/> </p><br />
<ol start="1" type="1"><br />
<li>Alternative surface sterilization technique; in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization. Considering the diseases and pandemics caused by gram negatives, (including the recent EHEC pandemic occurred in Europe), this possibility underlines the importance of LALF and similar factors.</li><br />
</ol><br />
<ol start="2" type="1"><br />
<li>Alternative cure for septic shock; after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.</li><br />
<li>Possible new structure of antibiotic-like drug; antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of the production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections. In addition, in biological laboratories, LALF may offer an alternative decontamination method.</li><br />
</ol><br />
<ol start="4" type="1"><br />
<li>Possibility of producing longtime sterile layers; if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In the case of a possible infection on surface, LALF will stop bacteria growth.</li><br />
<li>Alternative cure method for bacterial diseases; diseases like urinary system infection or gastritis are caused by gram negative bacteria. In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.</li><br />
</ol><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<ol start="1" type="1"><br />
<li>Camouflage material; this protein allows its host, cephalopod, to hide when cephalopod hunts or defends itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans</li><br />
<li>New material of dye for varying applications; Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<br /><br />
</li><br />
<li> Possibility of producing specialized light colors; in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<br /><br />
</li><br />
<li> It is planned that E.coli would synthesize reflectin while B.subtilis would produce LALF. Thus, as the growth of E.coli slows down and, finally, stops; thickness of layer of E.coli colonies would thinner than the thickness of B.subtilis layer. Depending on tinting ability of reflectin, we would observe the media in different colors that indicates the result very easily. This sample presents us a new biosensor method in prospective lab experiments.</li><br />
</ol><br />
<p>&nbsp;</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/9/94/Fp4.jpg" width="127" height="212"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;; <img src="https://static.igem.org/mediawiki/2011/b/b3/Fp2.jpg" alt="Fp2.jpg (250×159)" width="334" height="212"/></p><br />
<p>Beyond the above, we are also planning to</p><br />
<ol><br />
<ol><br />
<li>…purify B.subtilis biofilm with LALF via Fenton Reagent solution. Fenton Reagent is supposed to kill B.subtilis colonies and endospores. Alternative methods would make this model possible.</li><br />
<li>…see tinting ability of reflectin. Reflectin can be purified and observed with specific methods.</li><br />
</ol><br />
</ol><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Future_Plan
Team:Fatih Turkey/Future Plan
2011-10-29T01:22:34Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
ol{<br />
font-family: Verdana, Arial, SunSans-Regular, sans-serif;<br />
font-size: 12px;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Future Plan</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<p>Our project may suggest some useful ideas about varying applications that may be very crucial in the future.<br /><br />
For our Limulus anti lipopolysaccharide factor protein (LALF); some idea suggestions can be enumerated as:</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/67/Fp3.jpg" alt="Fp3.jpg (250×167)"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/68/Fp1.jpg"/> &nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/4/46/Fp5.jpg" alt="Fp5.jpg (250×188)" width="226" height="168"/> </p><br />
<ol start="1" type="1"><br />
<li>Alternative surface sterilization technique; in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization. Considering the diseases and pandemics caused by gram negatives, (including the recent EHEC pandemic occurred in Europe), this possibility underlines the importance of LALF and similar factors.</li><br />
</ol><br />
<ol start="2" type="1"><br />
<li>Alternative cure for septic shock; after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.</li><br />
<li>Possible new structure of antibiotic-like drug; antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of the production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections. In addition, in biological laboratories, LALF may offer an alternative decontamination method.</li><br />
</ol><br />
<ol start="4" type="1"><br />
<li>Possibility of producing longtime sterile layers; if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In the case of a possible infection on surface, LALF will stop bacteria growth.</li><br />
<li>Alternative cure method for bacterial diseases; diseases like urinary system infection or gastritis are caused by gram negative bacteria. In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.</li><br />
</ol><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<ol start="1" type="1"><br />
<li>Camouflage material; this protein allows its host, cephalopod, to hide when cephalopod hunts or defends itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans</li><br />
<li>New material of dye for varying applications; Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<br /><br />
</li><br />
<li> Possibility of producing specialized light colors; in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<br /><br />
</li><br />
<li> It is planned that E.coli would synthesize reflectin while B.subtilis would produce LALF. Thus, as the growth of E.coli slows down and, finally, stops; thickness of layer of E.coli colonies would thinner than the thickness of B.subtilis layer. Depending on tinting ability of reflectin, we would observe the media in different colors that indicates the result very easily. This sample presents us a new biosensor method in prospective lab experiments.</li><br />
</ol><br />
<p>&nbsp;</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/9/94/Fp4.jpg" width="127" height="212"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/b/b3/Fp2.jpg" alt="Fp2.jpg (250×159)" width="334" height="212"/></p><br />
<p>Beyond the above, we are also planning to</p><br />
<ol><br />
<ol><br />
<li>…purify B.subtilis biofilm with LALF via Fenton Reagent solution. Fenton Reagent is supposed to kill B.subtilis colonies and endospores. Alternative methods would make this model possible.</li><br />
<li>…see tinting ability of reflectin. Reflectin can be purified and observed with specific methods.</li><br />
</ol><br />
</ol><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Future_Plan
Team:Fatih Turkey/Future Plan
2011-10-29T01:21:55Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
ol{<br />
font-family: Verdana, Arial, SunSans-Regular, sans-serif;<br />
font-size: 12px;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Future Plan</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<p>Our project may suggest some useful ideas about varying applications that may be very crucial in the future.<br /><br />
For our Limulus anti lipopolysaccharide factor protein (LALF); some idea suggestions can be enumerated as:</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/67/Fp3.jpg" alt="Fp3.jpg (250×167)"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/68/Fp1.jpg"/> &nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/4/46/Fp5.jpg" alt="Fp5.jpg (250×188)" width="226" height="168"/> </p><br />
<ol start="1" type="1"><br />
<li>Alternative surface sterilization technique; in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization. Considering the diseases and pandemics caused by gram negatives, (including the recent EHEC pandemic occurred in Europe), this possibility underlines the importance of LALF and similar factors.</li><br />
</ol><br />
<ol start="2" type="1"><br />
<li>Alternative cure for septic shock; after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.</li><br />
<li>Possible new structure of antibiotic-like drug; antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of the production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections. In addition, in biological laboratories, LALF may offer an alternative decontamination method.</li><br />
</ol><br />
<ol start="4" type="1"><br />
<li>Possibility of producing longtime sterile layers; if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In the case of a possible infection on surface, LALF will stop bacteria growth.</li><br />
<li>Alternative cure method for bacterial diseases; diseases like urinary system infection or gastritis are caused by gram negative bacteria. In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.</li><br />
</ol><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<ol start="1" type="1"><br />
<li>Camouflage material; this protein allows its host, cephalopod, to hide when cephalopod hunts or defends itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans</li><br />
<li>New material of dye for varying applications; Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<br /><br />
</li><br />
<li> Possibility of producing specialized light colors; in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<br /><br />
</li><br />
<li> It is planned that E.coli would synthesize reflectin while B.subtilis would produce LALF. Thus, as the growth of E.coli slows down and, finally, stops; thickness of layer of E.coli colonies would thinner than the thickness of B.subtilis layer. Depending on tinting ability of reflectin, we would observe the media in different colors that indicates the result very easily. This sample presents us a new biosensor method in prospective lab experiments.</li><br />
</ol><br />
<p>&nbsp;</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/9/94/Fp4.jpg" width="127" height="212"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/b/b3/Fp2.jpg" alt="Fp2.jpg (250×159)" width="334" height="212"/></p><br />
<p>Beyond the above, we are also planning to</p><br />
<ol><br />
<ol><br />
<li>…purify B.subtilis biofilm with LALF via Fenton Reagent solution. Fenton Reagent is supposed to kill B.subtilis colonies and endospores. Alternative methods would make this model possible.</li><br />
<li>…see tinting ability of reflectin. Reflectin can be purified and observed with specific methods.</li><br />
</ol><br />
</ol><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Future_Plan
Team:Fatih Turkey/Future Plan
2011-10-29T01:21:03Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
ol{<br />
font-family: Verdana, Arial, SunSans-Regular, sans-serif;<br />
font-size: 12px;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Future Plan</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<p>Our project may suggest some useful ideas about varying applications that may be very crucial in the future.<br /><br />
For our Limulus anti lipopolysaccharide factor protein (LALF); some idea suggestions can be enumerated as:</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/6/67/Fp3.jpg" alt="Fp3.jpg (250×167)"/><img src="https://static.igem.org/mediawiki/2011/6/68/Fp1.jpg"/><img src="https://static.igem.org/mediawiki/2011/4/46/Fp5.jpg" alt="Fp5.jpg (250×188)" width="226" height="168"/> </p><br />
<ol start="1" type="1"><br />
<li>Alternative surface sterilization technique; in case of applying on surfaces, LALF protein can work like a new sterilization material like gluteraldehyte or alcohol that are commonly used for hospital and lab sterilization. Considering the diseases and pandemics caused by gram negatives, (including the recent EHEC pandemic occurred in Europe), this possibility underlines the importance of LALF and similar factors.</li><br />
</ol><br />
<ol start="2" type="1"><br />
<li>Alternative cure for septic shock; after gram negative bacterial infections; LPS, which is the main component of cell wall of gram negative bacteria, can cause deathly conditions that is generally called “septic shock”. In our project, our LALF protein binds this material strictly, thus LALF can be used as an alternative cure method for such condition.</li><br />
<li>Possible new structure of antibiotic-like drug; antibiotics commonly effect on production of cell wall of bacteria. This is done by inhibiting the source of the production. On the other hand, our LALF protein directly binds the cell wall material and stops the bacteria growth. Thus, a new antibiotic like drug made of LALF may be produced and used in some infections. In addition, in biological laboratories, LALF may offer an alternative decontamination method.</li><br />
</ol><br />
<ol start="4" type="1"><br />
<li>Possibility of producing longtime sterile layers; if any kind of object is produced by covering with LALF, that object can stay sterile for long time because of gram negative bacteria protection of LALF. In the case of a possible infection on surface, LALF will stop bacteria growth.</li><br />
<li>Alternative cure method for bacterial diseases; diseases like urinary system infection or gastritis are caused by gram negative bacteria. In the course of applying our bacteria that produce LALF in the infectious cite, infection of harmful bacteria can be prevented or stopped. This may suggest an alternative cure for such diseases.</li><br />
</ol><br />
<p>On the other hand, our secondary protein, reflectin, can be used in varying applications.</p><br />
<ol start="1" type="1"><br />
<li>Camouflage material; this protein allows its host, cephalopod, to hide when cephalopod hunts or defends itself from its hunters. In the course of development of reflectin protein, a new camouflage material may be produced for humans</li><br />
<li>New material of dye for varying applications; Dye that changes its color can be used almost every colorful application. From fireworks to wall paints, from windows of buildings to road signs, this ability can be very useful.<br /><br />
</li><br />
<li> Possibility of producing specialized light colors; in some circumstances, a unique light color may be necessary in order to gain maximum output like in greenhouses or dark rooms in photography industry. Reflectin reflects the light by changing its wavelength; therefore the light that is reflected has only one color. This ability can be applied in such application that mentioned above.<br /><br />
</li><br />
<li> It is planned that E.coli would synthesize reflectin while B.subtilis would produce LALF. Thus, as the growth of E.coli slows down and, finally, stops; thickness of layer of E.coli colonies would thinner than the thickness of B.subtilis layer. Depending on tinting ability of reflectin, we would observe the media in different colors that indicates the result very easily. This sample presents us a new biosensor method in prospective lab experiments.</li><br />
</ol><br />
<p>&nbsp;</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/9/94/Fp4.jpg" width="127" height="212"/>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/b/b3/Fp2.jpg" alt="Fp2.jpg (250×159)" width="334" height="212"/></p><br />
<p>Beyond the above, we are also planning to</p><br />
<ol><br />
<ol><br />
<li>…purify B.subtilis biofilm with LALF via Fenton Reagent solution. Fenton Reagent is supposed to kill B.subtilis colonies and endospores. Alternative methods would make this model possible.</li><br />
<li>…see tinting ability of reflectin. Reflectin can be purified and observed with specific methods.</li><br />
</ol><br />
</ol><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Reflectin
Team:Fatih Turkey/Reflectin
2011-10-29T01:13:16Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Reflectin</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<br />
<p><strong>Reflectin</strong><br /><br />
Many cephalopods (squid, cuttlefish, octopus etc.) exhibit remarkable dermal iridescence (more than meets the eye), a component of their complex, dynamic camouflage and communication. In the species Euprymna scolopes, the light organ iridescence is static and is due to reflectin protein-based platelets assembled into lamellar thin-film reflectors called iridosomes, contained within iridescent cells called iridocytes.<br /><br />
Reflectin hierarchical protein assembly is necessity for the responsive, tunable optical function of iridosome cells. This protein assembly can be triggered by chemical stimulation and that assembly can be reversible and fine-tuned.<br /><br />
Namely reflectin proteins were identified as the major biomaterial component of iridosomes. The RA1 gene in The Rainbow Graveyard Project was artificially synthesized into pBluescript to transform into E. coli. In addition, Kramer et al. (2007) isolated reflectin proteins exhibit unusual solubility and self-association properties.</p><br />
<p> <img src="https://static.igem.org/mediawiki/2011/2/20/Reflectinhkjljk..png" alt="Reflectinhkjljk..png (458×274)" /> <br /><br />
Self-association of RA1 is likely to stem from a combination of electrostatic and weak aromatic interactions. Reflectins are, indeed, characterized by their high content of polar aromatic residues and arginines, and several lines of evidence suggest that they are intrinsically unstructured, with no likely transmembrane, alphahelix or beta-sheet regions. E. scolopes reflectins also have high arginine, hydrophobic aromatic content and “methionine-rich membrane-associated proteins”. It is demonstrated that they have a high affinity for assembly with microsomal membranes.<br /><br />
The mechanism of reflectance is the same as that of colored soap bubbles. If the soap film (or multilayer plate) is very thin, shorter wavelengths are reflected, e.g. blue light; if it is thicker, longer wavelengths, such as yellow and red, are reflected. The reflectin proteins that apparently change their conformation or assembly to reversibly create the photonic structure.</p><br />
<p> <img src="https://static.igem.org/mediawiki/2011/1/19/Reflectn1ujh%C4%B1s.png" alt="Reflectn1ujhıs.png (451×222)" /></p><br />
<p>Multilayer reflector that appears red at near-normal viewing angles will appear first yellow, then green and blue at increasingly oblique angles. This dynamic reflection occurs by altering platelet and inter-platelet thicknesses in the multilayer reflector and/or altering the overall effective refractive index of the intra-platelet material. This allows the entire visible spectrum to be reflected from a single platelet stack. The increase in film thickness resulted in detectable redshift of the visible spectra and dominated any effect of decreasing refractive index owing to water sorption, which would have caused a blue-shift in the spectrum Kramer et al. (2007) have performed Micro-dialysis of reflectin 1a into various buffers, which resulted in two general types of aggregative structures. Optically clear bulk precipitation was seen in non-reducing conditions and filamentous protein structures were observed in reducing conditions controlled through the addition of a 10:1 ratio of reduced to oxidized glutathione .After several weeks at 4 ◦C, the filamentous structures formed a webbed structure that resulted in the supramolecular assembly of thin ribbons.</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/5/50/Reflectnkjmld%C5%9Fkas.png" alt="Reflectnkjmldşkas.png (405×445)" width="353" height="384" align="middle" /><img src="https://static.igem.org/mediawiki/2011/2/27/Squid_021005_42.png" alt="Squid_021005_42.jpg (500×386)" width="502" height="386" align="top" /><br /><br />
<br />
</p><br />
<p>In our project, we use reflectin 1A for detecting the survival of E.coli. Both of our bacteria (E.coli and B.subtilis) are expected to synthesize reflectin. As LALF is being produced by B.subtilis, both bacteria will synthesize reflectin. Meanwhile, LALF will bind to cell wall of gram negative bacterium (E.coli in our project). Therefore, gram (-) bacteria will not grow and survive in our biofilm surface. During the binding process, production of reflectin layer is going to decrease. Because of this, color of the surface will change due to the light reflecting ability of reflectin depending on the thickness of its layer. So, if the colour changes from red to blue, we can say there was a contact between E.coli and B.subtilis via anti-LPS factor.</p><br />
<br />
<br />
<br />
<h3>REFERENCES</h3><br />
<ol><br />
<li>nature materials VOL 6 JULY 2007<br />
(Review.The self-organizing properties of squid reflectin protein, Ryan M. Kramer et al.) <br />
Published online: 3 June 2007; doi:10.1038/nmat1930</li><br />
<li>J. R. Soc. Interface (2009) 6, S149–S163<br />
doi:10.1098/rsif.2008.0366.focus<br />
Published online 15 December 2008<br />
(Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S155)</li><br />
<li>Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S161</li><br />
<li>DOI: 10.1126/science.1091288<br />
Science 303, 235 (2004);<br />
Wendy J. Crookes, et al.(www.sciencemag.org SCIENCE VOL303 9 JANUARY 2004)</li><br />
<li>A.R. Tao et al. / Biomaterials 31 (2010) 793–801</li><br />
<li>J. R. Soc. Interface (2010) 7, 549–560 <br />
doi:10.1098/rsif.2009.0299<br />
Published online 23 September 2009(Reflectin phosphorylation M. Izumi et al.)</li><br />
</ol><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Reflectin
Team:Fatih Turkey/Reflectin
2011-10-29T01:12:18Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Reflectin</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<br />
<p><strong>Reflectin</strong><br /><br />
Many cephalopods (squid, cuttlefish, octopus etc.) exhibit remarkable dermal iridescence (more than meets the eye), a component of their complex, dynamic camouflage and communication. In the species Euprymna scolopes, the light organ iridescence is static and is due to reflectin protein-based platelets assembled into lamellar thin-film reflectors called iridosomes, contained within iridescent cells called iridocytes.<br /><br />
Reflectin hierarchical protein assembly is necessity for the responsive, tunable optical function of iridosome cells. This protein assembly can be triggered by chemical stimulation and that assembly can be reversible and fine-tuned.<br /><br />
Namely reflectin proteins were identified as the major biomaterial component of iridosomes. The RA1 gene in The Rainbow Graveyard Project was artificially synthesized into pBluescript to transform into E. coli. In addition, Kramer et al. (2007) isolated reflectin proteins exhibit unusual solubility and self-association properties.</p><br />
<p> <img src="https://static.igem.org/mediawiki/2011/2/20/Reflectinhkjljk..png" alt="Reflectinhkjljk..png (458×274)" /> <br /><br />
Self-association of RA1 is likely to stem from a combination of electrostatic and weak aromatic interactions. Reflectins are, indeed, characterized by their high content of polar aromatic residues and arginines, and several lines of evidence suggest that they are intrinsically unstructured, with no likely transmembrane, alphahelix or beta-sheet regions. E. scolopes reflectins also have high arginine, hydrophobic aromatic content and “methionine-rich membrane-associated proteins”. It is demonstrated that they have a high affinity for assembly with microsomal membranes.<br /><br />
The mechanism of reflectance is the same as that of colored soap bubbles. If the soap film (or multilayer plate) is very thin, shorter wavelengths are reflected, e.g. blue light; if it is thicker, longer wavelengths, such as yellow and red, are reflected. The reflectin proteins that apparently change their conformation or assembly to reversibly create the photonic structure.</p><br />
<p> <img src="https://static.igem.org/mediawiki/2011/1/19/Reflectn1ujh%C4%B1s.png" alt="Reflectn1ujhıs.png (451×222)" /></p><br />
<p>Multilayer reflector that appears red at near-normal viewing angles will appear first yellow, then green and blue at increasingly oblique angles. This dynamic reflection occurs by altering platelet and inter-platelet thicknesses in the multilayer reflector and/or altering the overall effective refractive index of the intra-platelet material. This allows the entire visible spectrum to be reflected from a single platelet stack. The increase in film thickness resulted in detectable redshift of the visible spectra and dominated any effect of decreasing refractive index owing to water sorption, which would have caused a blue-shift in the spectrum Kramer et al. (2007) have performed Micro-dialysis of reflectin 1a into various buffers, which resulted in two general types of aggregative structures. Optically clear bulk precipitation was seen in non-reducing conditions and filamentous protein structures were observed in reducing conditions controlled through the addition of a 10:1 ratio of reduced to oxidized glutathione .After several weeks at 4 ◦C, the filamentous structures formed a webbed structure that resulted in the supramolecular assembly of thin ribbons.</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/5/50/Reflectnkjmld%C5%9Fkas.png" alt="Reflectnkjmldşkas.png (405×445)" width="353" height="384" align="middle" /><img src="https://static.igem.org/mediawiki/2011/2/27/Squid_021005_42.png" alt="Squid_021005_42.jpg (500×386)" width="512" height="394" align="top" /><br /><br />
</p><br />
<p>In our project, we use reflectin 1A for detecting the survival of E.coli. Both of our bacteria (E.coli and B.subtilis) are expected to synthesize reflectin. As LALF is being produced by B.subtilis, both bacteria will synthesize reflectin. Meanwhile, LALF will bind to cell wall of gram negative bacterium (E.coli in our project). Therefore, gram (-) bacteria will not grow and survive in our biofilm surface. During the binding process, production of reflectin layer is going to decrease. Because of this, color of the surface will change due to the light reflecting ability of reflectin depending on the thickness of its layer. So, if the colour changes from red to blue, we can say there was a contact between E.coli and B.subtilis via anti-LPS factor.</p><br />
<br />
<br />
<br />
<h3>REFERENCES</h3><br />
<ol><br />
<li>nature materials VOL 6 JULY 2007<br />
(Review.The self-organizing properties of squid reflectin protein, Ryan M. Kramer et al.) <br />
Published online: 3 June 2007; doi:10.1038/nmat1930</li><br />
<li>J. R. Soc. Interface (2009) 6, S149–S163<br />
doi:10.1098/rsif.2008.0366.focus<br />
Published online 15 December 2008<br />
(Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S155)</li><br />
<li>Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S161</li><br />
<li>DOI: 10.1126/science.1091288<br />
Science 303, 235 (2004);<br />
Wendy J. Crookes, et al.(www.sciencemag.org SCIENCE VOL303 9 JANUARY 2004)</li><br />
<li>A.R. Tao et al. / Biomaterials 31 (2010) 793–801</li><br />
<li>J. R. Soc. Interface (2010) 7, 549–560 <br />
doi:10.1098/rsif.2009.0299<br />
Published online 23 September 2009(Reflectin phosphorylation M. Izumi et al.)</li><br />
</ol><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Reflectin
Team:Fatih Turkey/Reflectin
2011-10-29T01:11:02Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Reflectin</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<p><strong>Reflectin</strong><br /><br />
Many cephalopods (squid, cuttlefish, octopus etc.) exhibit remarkable dermal iridescence (more than meets the eye), a component of their complex, dynamic camouflage and communication. In the species Euprymna scolopes, the light organ iridescence is static and is due to reflectin protein-based platelets assembled into lamellar thin-film reflectors called iridosomes, contained within iridescent cells called iridocytes.<br /><br />
Reflectin hierarchical protein assembly is necessity for the responsive, tunable optical function of iridosome cells. This protein assembly can be triggered by chemical stimulation and that assembly can be reversible and fine-tuned.<br /><br />
Namely reflectin proteins were identified as the major biomaterial component of iridosomes. The RA1 gene in The Rainbow Graveyard Project was artificially synthesized into pBluescript to transform into E. coli. In addition, Kramer et al. (2007) isolated reflectin proteins exhibit unusual solubility and self-association properties.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/2/20/Reflectinhkjljk..png" alt="Reflectinhkjljk..png (458×274)"/> <br /><br />
Self-association of RA1 is likely to stem from a combination of electrostatic and weak aromatic interactions. Reflectins are, indeed, characterized by their high content of polar aromatic residues and arginines, and several lines of evidence suggest that they are intrinsically unstructured, with no likely transmembrane, alphahelix or beta-sheet regions. E. scolopes reflectins also have high arginine, hydrophobic aromatic content and “methionine-rich membrane-associated proteins”. It is demonstrated that they have a high affinity for assembly with microsomal membranes.<br /><br />
The mechanism of reflectance is the same as that of colored soap bubbles. If the soap film (or multilayer plate) is very thin, shorter wavelengths are reflected, e.g. blue light; if it is thicker, longer wavelengths, such as yellow and red, are reflected. The reflectin proteins that apparently change their conformation or assembly to reversibly create the photonic structure.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/1/19/Reflectn1ujh%C4%B1s.png" alt="Reflectn1ujhıs.png (451×222)"/> </p><br />
<p>Multilayer reflector that appears red at near-normal viewing angles will appear first yellow, then green and blue at increasingly oblique angles. This dynamic reflection occurs by altering platelet and inter-platelet thicknesses in the multilayer reflector and/or altering the overall effective refractive index of the intra-platelet material. This allows the entire visible spectrum to be reflected from a single platelet stack. The increase in film thickness resulted in detectable redshift of the visible spectra and dominated any effect of decreasing refractive index owing to water sorption, which would have caused a blue-shift in the spectrum Kramer et al. (2007) have performed Micro-dialysis of reflectin 1a into various buffers, which resulted in two general types of aggregative structures. Optically clear bulk precipitation was seen in non-reducing conditions and filamentous protein structures were observed in reducing conditions controlled through the addition of a 10:1 ratio of reduced to oxidized glutathione .After several weeks at 4 ◦C, the filamentous structures formed a webbed structure that resulted in the supramolecular assembly of thin ribbons.</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/5/50/Reflectnkjmld%C5%9Fkas.png" alt="Reflectnkjmldşkas.png (405×445)" width="429" height="469" align="middle"/><img src="https://static.igem.org/mediawiki/2011/2/27/Squid_021005_42.png" alt="Squid_021005_42.jpg (500×386)" width="512" height="394" align="top"/><br /><br />
</p><br />
<p>In our project, we use reflectin 1A for detecting the survival of E.coli. Both of our bacteria (E.coli and B.subtilis) are expected to synthesize reflectin. As LALF is being produced by B.subtilis, both bacteria will synthesize reflectin. Meanwhile, LALF will bind to cell wall of gram negative bacterium (E.coli in our project). Therefore, gram (-) bacteria will not grow and survive in our biofilm surface. During the binding process, production of reflectin layer is going to decrease. Because of this, color of the surface will change due to the light reflecting ability of reflectin depending on the thickness of its layer. So, if the colour changes from red to blue, we can say there was a contact between E.coli and B.subtilis via anti-LPS factor.</p><br />
<br />
<br />
<h3>REFERENCES</h3><br />
<ol><br />
<li>nature materials VOL 6 JULY 2007<br />
(Review.The self-organizing properties of squid reflectin protein, Ryan M. Kramer et al.) <br />
Published online: 3 June 2007; doi:10.1038/nmat1930</li><br />
<li>J. R. Soc. Interface (2009) 6, S149–S163<br />
doi:10.1098/rsif.2008.0366.focus<br />
Published online 15 December 2008<br />
(Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S155)</li><br />
<li>Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S161</li><br />
<li>DOI: 10.1126/science.1091288<br />
Science 303, 235 (2004);<br />
Wendy J. Crookes, et al.(www.sciencemag.org SCIENCE VOL303 9 JANUARY 2004)</li><br />
<li>A.R. Tao et al. / Biomaterials 31 (2010) 793–801</li><br />
<li>J. R. Soc. Interface (2010) 7, 549–560 <br />
doi:10.1098/rsif.2009.0299<br />
Published online 23 September 2009(Reflectin phosphorylation M. Izumi et al.)</li><br />
</ol><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Reflectin
Team:Fatih Turkey/Reflectin
2011-10-29T01:10:26Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Reflectin</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<p><strong>Reflectin</strong><br /><br />
Many cephalopods (squid, cuttlefish, octopus etc.) exhibit remarkable dermal iridescence (more than meets the eye), a component of their complex, dynamic camouflage and communication. In the species Euprymna scolopes, the light organ iridescence is static and is due to reflectin protein-based platelets assembled into lamellar thin-film reflectors called iridosomes, contained within iridescent cells called iridocytes.<br /><br />
Reflectin hierarchical protein assembly is necessity for the responsive, tunable optical function of iridosome cells. This protein assembly can be triggered by chemical stimulation and that assembly can be reversible and fine-tuned.<br /><br />
Namely reflectin proteins were identified as the major biomaterial component of iridosomes. The RA1 gene in The Rainbow Graveyard Project was artificially synthesized into pBluescript to transform into E. coli. In addition, Kramer et al. (2007) isolated reflectin proteins exhibit unusual solubility and self-association properties.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/2/20/Reflectinhkjljk..png" alt="Reflectinhkjljk..png (458×274)"/> <br /><br />
Self-association of RA1 is likely to stem from a combination of electrostatic and weak aromatic interactions. Reflectins are, indeed, characterized by their high content of polar aromatic residues and arginines, and several lines of evidence suggest that they are intrinsically unstructured, with no likely transmembrane, alphahelix or beta-sheet regions. E. scolopes reflectins also have high arginine, hydrophobic aromatic content and “methionine-rich membrane-associated proteins”. It is demonstrated that they have a high affinity for assembly with microsomal membranes.<br /><br />
The mechanism of reflectance is the same as that of colored soap bubbles. If the soap film (or multilayer plate) is very thin, shorter wavelengths are reflected, e.g. blue light; if it is thicker, longer wavelengths, such as yellow and red, are reflected. The reflectin proteins that apparently change their conformation or assembly to reversibly create the photonic structure.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/1/19/Reflectn1ujh%C4%B1s.png" alt="Reflectn1ujhıs.png (451×222)"/> </p><br />
<p>Multilayer reflector that appears red at near-normal viewing angles will appear first yellow, then green and blue at increasingly oblique angles. This dynamic reflection occurs by altering platelet and inter-platelet thicknesses in the multilayer reflector and/or altering the overall effective refractive index of the intra-platelet material. This allows the entire visible spectrum to be reflected from a single platelet stack. The increase in film thickness resulted in detectable redshift of the visible spectra and dominated any effect of decreasing refractive index owing to water sorption, which would have caused a blue-shift in the spectrum Kramer et al. (2007) have performed Micro-dialysis of reflectin 1a into various buffers, which resulted in two general types of aggregative structures. Optically clear bulk precipitation was seen in non-reducing conditions and filamentous protein structures were observed in reducing conditions controlled through the addition of a 10:1 ratio of reduced to oxidized glutathione .After several weeks at 4 ◦C, the filamentous structures formed a webbed structure that resulted in the supramolecular assembly of thin ribbons.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/5/50/Reflectnkjmld%C5%9Fkas.png" alt="Reflectnkjmldşkas.png (405×445)" width="429" height="469" align="middle"/><img src="https://static.igem.org/mediawiki/2011/2/27/Squid_021005_42.png" alt="Squid_021005_42.jpg (500×386)" width="512" height="394" align="top"/><br /><br />
</p><br />
<p>In our project, we use reflectin 1A for detecting the survival of E.coli. Both of our bacteria (E.coli and B.subtilis) are expected to synthesize reflectin. As LALF is being produced by B.subtilis, both bacteria will synthesize reflectin. Meanwhile, LALF will bind to cell wall of gram negative bacterium (E.coli in our project). Therefore, gram (-) bacteria will not grow and survive in our biofilm surface. During the binding process, production of reflectin layer is going to decrease. Because of this, color of the surface will change due to the light reflecting ability of reflectin depending on the thickness of its layer. So, if the colour changes from red to blue, we can say there was a contact between E.coli and B.subtilis via anti-LPS factor.</p><br />
<br />
<br />
<h3>REFERENCES</h3><br />
<ol><br />
<li>nature materials VOL 6 JULY 2007<br />
(Review.The self-organizing properties of squid reflectin protein, Ryan M. Kramer et al.) <br />
Published online: 3 June 2007; doi:10.1038/nmat1930</li><br />
<li>J. R. Soc. Interface (2009) 6, S149–S163<br />
doi:10.1098/rsif.2008.0366.focus<br />
Published online 15 December 2008<br />
(Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S155)</li><br />
<li>Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S161</li><br />
<li>DOI: 10.1126/science.1091288<br />
Science 303, 235 (2004);<br />
Wendy J. Crookes, et al.(www.sciencemag.org SCIENCE VOL303 9 JANUARY 2004)</li><br />
<li>A.R. Tao et al. / Biomaterials 31 (2010) 793–801</li><br />
<li>J. R. Soc. Interface (2010) 7, 549–560 <br />
doi:10.1098/rsif.2009.0299<br />
Published online 23 September 2009(Reflectin phosphorylation M. Izumi et al.)</li><br />
</ol><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Notebook13
Team:Fatih Turkey/Notebook13
2011-10-29T01:09:37Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Lab Garage</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Notebook</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<br />
<style><br />
#haftalar li{<br />
width: 20px;<br />
text-align: center;<br />
margin: 0 20px;<br />
float: left;<br />
list-style: none;<br />
cursor: pointer;<br />
font-size: 14px;<br />
font-weight:bold;<br />
border-radius: 6px;<br />
}<br />
<br />
#haftalar li:hover{<br />
background: #CCC;<br />
}<br />
<br />
#haftalar li.active{<br />
background: #CCC;<br />
}<br />
</style><br />
<br />
<br />
<div style="margin-left: 40px;"><br />
<span style="float: left;">Week: </span><br />
<ul id="haftalar"><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">1</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook2">2</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook3">3</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook4">4</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook5">5</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook6">6</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook7">7</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook8">8</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook9">9</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook10">10</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook11">11</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook12">12</a></li><br />
<li class="active"><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook13">13</a></li><br />
</ul><br />
</div><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="width: 840px; border: solid 3px #666; border-radius: 10px; padding-left: 30px;padding-right: 30px; margin:center "><br />
<p>...</p><br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Notebook12
Team:Fatih Turkey/Notebook12
2011-10-29T01:09:06Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Lab Garage</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Notebook</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<br />
<style><br />
#haftalar li{<br />
width: 20px;<br />
text-align: center;<br />
margin: 0 20px;<br />
float: left;<br />
list-style: none;<br />
cursor: pointer;<br />
font-size: 14px;<br />
font-weight:bold;<br />
border-radius: 6px;<br />
}<br />
<br />
#haftalar li:hover{<br />
background: #CCC;<br />
}<br />
<br />
#haftalar li.active{<br />
background: #CCC;<br />
}<br />
</style><br />
<br />
<br />
<div style="margin-left: 40px;"><br />
<span style="float: left;">Week: </span><br />
<ul id="haftalar"><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">1</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook2">2</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook3">3</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook4">4</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook5">5</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook6">6</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook7">7</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook8">8</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook9">9</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook10">10</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook11">11</a></li><br />
<li class="active"><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook12">12</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook13">13</a></li><br />
</ul><br />
</div><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="width: 840px; border: solid 3px #666; border-radius: 10px; padding-left: 30px;padding-right: 30px; margin:center "><br />
<p>Holland</p><br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Notebook11
Team:Fatih Turkey/Notebook11
2011-10-29T01:08:44Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Lab Garage</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Notebook</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<br />
<style><br />
#haftalar li{<br />
width: 20px;<br />
text-align: center;<br />
margin: 0 20px;<br />
float: left;<br />
list-style: none;<br />
cursor: pointer;<br />
font-size: 14px;<br />
font-weight:bold;<br />
border-radius: 6px;<br />
}<br />
<br />
#haftalar li:hover{<br />
background: #CCC;<br />
}<br />
<br />
#haftalar li.active{<br />
background: #CCC;<br />
}<br />
</style><br />
<br />
<br />
<div style="margin-left: 40px;"><br />
<span style="float: left;">Week: </span><br />
<ul id="haftalar"><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">1</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook2">2</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook3">3</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook4">4</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook5">5</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook6">6</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook7">7</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook8">8</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook9">9</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook10">10</a></li><br />
<li class="active"><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook11">11</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook12">12</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook13">13</a></li><br />
</ul><br />
</div><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="width: 840px; border: solid 3px #666; border-radius: 10px; padding-left: 30px;padding-right: 30px; margin:center "><br />
<p>presentation</p><br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Notebook10
Team:Fatih Turkey/Notebook10
2011-10-29T01:07:22Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Lab Garage</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Notebook</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<br />
<style><br />
#haftalar li{<br />
width: 20px;<br />
text-align: center;<br />
margin: 0 20px;<br />
float: left;<br />
list-style: none;<br />
cursor: pointer;<br />
font-size: 14px;<br />
font-weight:bold;<br />
border-radius: 6px;<br />
}<br />
<br />
#haftalar li:hover{<br />
background: #CCC;<br />
}<br />
<br />
#haftalar li.active{<br />
background: #CCC;<br />
}<br />
</style><br />
<br />
<br />
<div style="margin-left: 40px;"><br />
<span style="float: left;">Week: </span><br />
<ul id="haftalar"><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">1</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook2">2</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook3">3</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook4">4</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook5">5</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook6">6</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook7">7</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook8">8</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook9">9</a></li><br />
<li class="active"><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook10">10</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook11">11</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook12">12</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook13">13</a></li><br />
</ul><br />
</div><br />
<br />
<br />
</div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="width: 840px; border: solid 3px #666; border-radius: 10px; padding-left: 30px;padding-right: 30px; margin:center "><br />
<p>wiki</p><br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/LALF
Team:Fatih Turkey/LALF
2011-10-29T01:06:18Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">LALF (Anti-LPS)</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"><br />
<br />
<br />
<br />
<br />
<p>As we know, seawater is a virtual &quot;bacterial soup&quot;. Typical near-shore areas that form the prime habitat of the horseshoe crab can easily contain over one billion Gram-negative bacteria per milliliter of seawater. Thus, the horseshoe crab is constantly threatened with infection. Unlike mammals, including humans, the horseshoe crab lacks an immune system; it cannot develop antibodies to fight infection. However, the horseshoe crab does contain a number of compounds that will bind to and inactivate bacteria, fungi, and viruses. Anti-LPS factors that are synthesized in the blood cells of crab are part of this primitive &quot;immune&quot; system.<br /><br />
Lots of horseshoe crabs are collected by some manufacture companies and their blood is extracted in some laboratories. During the extraction process, up to 30% of the animal's blood is removed. Research has shown that once returned to the water, the horseshoe crab's blood volume rebounds in about a week. But, it is also noted that a number of crabs which cannot be undervalued are also dying during the process.<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/2/2d/590_crash_blood.png" alt="590_crash_blood.png (613×359)"/> <br /><br />
</p><br />
<p>In our project, we aim to produce those factors by using the synthetic biology. In this way, we can obtain them without extracting blood and hurting any crab. Additionally, the possible medication will be gathered cheaper.<br /><br />
In our project, mainly we study on a protein that is gathered from horseshoe crab (<em>limulus polyphemus), </em>limulus anti lipopolysaccharide factor (LALF). By the help of this protein, we planned to stop bacteria growth in vitro situations. </p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/1/1b/Ppk%C4%B1jougj.png" alt="Ppkıjougj.png (393×369)" width="381" height="357"/></p><br />
<p>Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge-shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock.<br /><br />
To make it possible, we use Bacillus Subtilis as a host; because it is supposed that this bacterium cannot be affected by LALF. Preventing such possibilities during our experiments will help us to get the best and the clearest results. <br /><br />
On the other hand, we want to apply our anti-LPS factor on a surface as a coat in order to obtain an anti-gram negative bacterial surface. Normally, B.subtilis has the ability to produce biofilm. This complex media may also include some components and protein that are synthesized by bacteria. We think that after the production of LALF, the protein can pass to biofilm with the help of signal peptides we added. As long as LALF remains in the biofilm, the surface that is covered with that biofilm material will not be infected by gram negatives.</p><br />
<p><img src="https://static.igem.org/mediawiki/2011/4/4d/Mustafa--lalf.png" alt="Mustafa--lalf.png (610×496)" width="550" height="440"/><img src="https://static.igem.org/mediawiki/2011/c/c9/Gjhjg%C3%B6hk%C3%A7j..png" alt="Gjhjgöhkçj..png (592×956)" width="274" height="442"/><br /><br />
</p><br />
<p>The LPS coat of gram negative bacteria is an important reason of endotoxin and septic shock. LALF binds to that LPS coat and inhibits the growth of bacteria. This ability is very crucial and may be very useful considering the diseases and pandemics that are caused by gram negatives. Our project suggests a possible prevention for such circumstances.<br /><br />
Also, by killing spores of B.subtilis on the biofilm surface with aqueous dissolved oxygen, ascorbic acid, and copper ions, we will try to perform a sterilized coating material which will possess a protective feature against infectious gram negative bacteria. </p><br />
<br />
<br />
<br />
<h3>REFERENCES</h3><br />
<ol><br />
<li>Alpert G, Baldwin G, Thompson C, Wainwright N, Novitsky T J, Gillis , Parsonnet J, Fleisher G R, Siber G R. Limulus antilipopolysaccharide<br />
factor protects rabbits from meningococcal endotoxin shock. J Infect Dis. 1992;165:494–500. [PubMed]</li><br />
<li>Bannerman D D, Goldblum S E. Endotoxin induces endothelial barrier dysfunction through protein tyrosine phosphorylation. Am J Physiol. 1997;273:L217–L226. [PubMed]<br />
</li><br />
<li>Battafaraono R J, Dahlberg P S, Ratz C A, Johnston J W, Gray B H, Haseman J R, Mayo K H, Dunn D L. Peptide derivatives of three distinct<br />
lipopolysaccharide binding proteins inhibit lipopolysaccharide-induced tumor necrosis factor-alpha secretion in vitro. Surgery. 1995;118:318–324. [PubMed]</li><br />
<li>Cooperstock M S. Inactivation of endotoxin by polymyxin B. Antimicrob Agents Chemother. 1974;6:422–425. [PMC free article] [PubMed]</li><br />
<li>Evans T J, Carpenter A, Moyes D, Martin R, Cohen J. Protective effects of a recombinant amino-terminal fragment of human<br />
bactericidal/permeability-increasing protein in an animal model of gram negative sepsis. J Infect Dis. 1995;171:153–160. [PubMed]</li><br />
<li>Fletcher M A, Mckena T M, Quance J L, Wainwright N R, Williams T J. Lipopolysaccharide detoxification by endotoxin neutralizing protein. J<br />
Surg Res. 1993;55:147–154. [PubMed]</li><br />
<li>Frey E A, Miller D S, Jahr T G, Sundan A, Bazil V, Espevik T, Finlay B B, Wright S D. Soluble CD14 participates in the response of cells to<br />
lipopolysaccharide. J Exp Med. 1992;176:1665–1671. [PMC free article] [PubMed]</li><br />
<li>Goldblum S E, Brann T W, Ding X, Pugin J, Tobias P S. Lipopolysaccharide (LPS)-binding protein and soluble CD14 function as accessory molecules for LPS-induced changes in endothelial barrier function, in vitro. J Clin Invest. 1994;93:692–702. [PMC free article] [PubMed]</li><br />
<li>Goldblum S E, Ding X, Brann T W, Campbell-Washington J. Bacterial lipopolysaccharide induces actin reorganization, intercellular gap<br />
formation, and endothelial barrier dysfunction in pulmonary vascular endothelial cells: concurrent F-actin depolymerization and new actin<br />
synthesis. J Cell Physiol. 1993;157:13–23. [PubMed]</li><br />
<li>Hirata M, Zhong J, Wright S C, Larrick J W. Structure and functions of endotoxin-binding peptides derived from CAP18. Prog Clin Biol Res.<br />
1995;392:317–326. [PubMed]</li><br />
<li>Hoess A, Watson S, Siber G R, Liddington R. Crystal structure of endotoxin-neutralizing protein from the horseshoe crab, Limulus anti-LPS<br />
factor, at 1.5 A resolution. EMBO J. 1993;12:3351–3356. [PMC free article] [PubMed]</li><br />
<li>Levine D M, Parker T S, Donelly T M, Walsh A, Rubin A L. In vivo protection against endotoxin by plasma high density lipoprotein. Proc Natl<br />
Acad Sci USA. 1993;90:12040–12044. [PMC free article] [PubMed]</li><br />
<li>Morita T, Ohtsubo S, Nakamura T, Tanaka S, Iwanaga S, Ohashi K, Niwa M. Isolation and biological activities of limulus anticoagulant<br />
(anti-LPS factor) which interacts with lipopolysaccharide (LPS) J Biochem (Tokyo) 1985;97:1611–1620. [PubMed]</li><br />
<li>Morrison D C, Jacobs D M. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry.<br />
1976;13:813–818. [PubMed]</li><br />
<li>Muta T, Miyata T, Tokunaga F, Nakamura T, Iwanaga S. Primary structure of anti-lipopolysaccharide factor from American horseshoe crab,<br />
Limulus polyphemus. J Biochem (Tokyo) 1987;101:1321–1330. [PubMed]</li><br />
<li>Netea M G, Demacker P N M, Kullberg B J, Boerman O C, Verschueren I, Stalenhoef A F H, van der Meer J W M. Low-density lipoprotein<br />
receptor-deficient mice are protected against lethal endotoxemia and severe Gram-negative infections. J Clin Invest. 1996;97:1366–1372.<br />
[PMC free article] [PubMed]</li><br />
<li>Ulevitch R J, Tobias P S. Recognition of endotoxin by cells leading to transmembrane signaling. Curr Opin Immunol. 1994;6:125–130.<br />
[PubMed]<br />
</li><br />
<li>Wainwright N R, Miller R J, Paus E, Novitsky T J, Fletcher M A, McKenna T M, Williams T. Endotoxin binding and neutralizing activity by a<br />
protein from Limulus polyphemus. In: Levin J, Alving C R, Munford R S, Stutz P L, editors. Cellular and molecular aspects of endotoxin<br />
reactions. New York, N.Y: Elsevier Science Publishers; 1990. pp. 315–325.</li><br />
<li>Warren H S, Novitsky T J, Bucklin A, Kania S A, Siber G R. Endotoxin neutralization with rabbit antisera to Escherichia coli J5 and other<br />
gram-negative bacteria. Infect Immun. 1987;55:1668–1673. [PMC free article] [PubMed]</li><br />
<li>Weinstein S L, June C H, DeFranco A L. Lipopolysaccharide-induced protein tyrosine phosphorylation in human macrophages is mediated<br />
by CD14. J Immunol. 1993;151:3829–3838. [PubMed]</li><br />
<li>A. Hoess, S. Watson, G.R.Siber and R.Liddington.Crystal structure of an endotoxin-neutralizing protein from the horseshoe crab, Limulus anti-LPS factor, at 1.5 A resolution.</li><br />
<li>Lihua Wu, Chao-Ming Tsai and Carl E. Frasch.A method purification of bacterial R-type LPS.</li><br />
<li>Kloczewiak, M., Black, K. M., Loiselle, P., Cavaillon, J. M., Wainwright, N., and Warren, H. S. (1994) J. Infect. Dis. 170, 1490–1497</li><br />
<li>http://www.horseshoecrab.org</li><br />
</ol><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/File:Mustafa--lalf.png
File:Mustafa--lalf.png
2011-10-29T00:59:27Z
<p>Fazilet: uploaded a new version of &quot;File:Mustafa--lalf.png&quot;</p>
<hr />
<div></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/confirmation
Team:Fatih Turkey/confirmation
2011-10-29T00:58:41Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Experiments</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Charactarization</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="float: left; width: 220px; border-radius: 10px; position: absolute; top: 50px; left: 0; background: #EEE; border: solid 3px #666;"><br />
<ol><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/confirmation">Charactarization</a></li><br />
<br />
</li><br />
<br />
<br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments1">Exposure Experiment</a></li><br />
<br />
<br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiment3C">Inhibition in Liquid Culture Assay</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments4">Inhibition in Supernatant Assay</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments2">The Suicide Experiment of E.Coli</a></li> <br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments5">Fenton Reagent Assay</a></li><br />
<li type="A"><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments6">Existence of Reflectin</a></li><br />
<br />
</ol><br />
</div><br />
<div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;"><br />
<table width="606" height="203" border="0"><br />
<tr><br />
<td width="600" valign="top"><p><strong>CHARACTERIZATION</strong><br /><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/5/59/Chrac.png" width="500" /></center></p><br />
<p>Before starting assays; we needed to be sure that our genes had been cloned correctly. In order to use our parts in our experiments, we confirmed our parts via electrophoresis. Following parts are fully confirmed:</p><br />
<table border="1" cellspacing="0" cellpadding="0"><br />
<tr><br />
<td width="307" height="35" valign="top"><strong>Subparts</strong> </td><br />
<td width="307" valign="top"><p><strong>Parts</strong></p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541501</p></td><br />
<td width="307" valign="top"><p>BBa_K541515</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541502</p></td><br />
<td width="307" valign="top"><p>BBa_K541545</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541503</p></td><br />
<td width="307" valign="top"><p>BBa_K541596</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541504</p></td><br />
<td width="307" valign="top"><p>BBa_K541915</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541505</p></td><br />
<td width="307" valign="top"><p>BBa_K541800</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>BBa_K541506</p></td><br />
<td width="307" valign="top"><p>BBa_K541516</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>&nbsp;</p></td><br />
<td width="307" valign="top"><p>BBa_K541526</p></td><br />
</tr><br />
<tr><br />
<td width="307" valign="top"><p>&nbsp;</p></td><br />
<td width="307" valign="top"><p>BBa_K541546</p></td><br />
</tr><br />
</table><br />
<p>After electrophoresis results; we furthermore performed sequencing and imaging tests to characterize our BioBricks. For BBa_541505 and BBa_541506; we added a GFP sequence and transformated into E.coli. This has characterized our main proteins. cDNA sequencing for these parts was also done successfully.<br /><br />
In addition, BBa_541800 is transformated into B.subtilis and successfully imaged red that shows RFP region and therefore plasmid backbone works properly.<br /><br />
We characterized BBa_K541545, BBa_K541515 and BBa_K541915 via sequencing.</p></td><br />
</tr><br />
</table><br />
<p><center><img src="https://static.igem.org/mediawiki/2011/b/bc/Jelgoruntusu.jpg" width="500" /></center></p><br />
<br><br />
<br><br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/File:Jelgoruntusu.jpg
File:Jelgoruntusu.jpg
2011-10-29T00:56:22Z
<p>Fazilet: </p>
<hr />
<div></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/LALF
Team:Fatih Turkey/LALF
2011-10-29T00:47:21Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">LALF (Anti-LPS)</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"><br />
<br />
<br />
<p>As we know, seawater is a virtual &quot;bacterial soup&quot;. Typical near-shore areas that form the prime habitat of the horseshoe crab can easily contain over one billion Gram-negative bacteria per milliliter of seawater. Thus, the horseshoe crab is constantly threatened with infection. Unlike mammals, including humans, the horseshoe crab lacks an immune system; it cannot develop antibodies to fight infection. However, the horseshoe crab does contain a number of compounds that will bind to and inactivate bacteria, fungi, and viruses. Anti-LPS factors that are synthesized in the blood cells of crab are part of this primitive &quot;immune&quot; system.<br /><br />
Lots of horseshoe crabs are collected by some manufacture companies and their blood is extracted in some laboratories. During the extraction process, up to 30% of the animal's blood is removed. Research has shown that once returned to the water, the horseshoe crab's blood volume rebounds in about a week. But, it is also noted that a number of crabs which cannot be undervalued are also dying during the process.<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/2/2d/590_crash_blood.png" alt="590_crash_blood.png (613×359)"/> <br /><br />
</p><br />
<p>In our project, we aim to produce those factors by using the synthetic biology. In this way, we can obtain them without extracting blood and hurting any crab. Additionally, the possible medication will be gathered cheaper.<br /><br />
In our project, mainly we study on a protein that is gathered from horseshoe crab (<em>limulus polyphemus), </em>limulus anti lipopolysaccharide factor (LALF). By the help of this protein, we planned to stop bacteria growth in vitro situations. <br /><br />
Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge-shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock.<br /><br />
To make it possible, we use Bacillus Subtilis as a host; because it is supposed that this bacterium cannot be affected by LALF. Preventing such possibilities during our experiments will help us to get the best and the clearest results. <br /><br />
On the other hand, we want to apply our anti-LPS factor on a surface as a coat in order to obtain an anti-gram negative bacterial surface. Normally, B.subtilis has the ability to produce biofilm. This complex media may also include some components and protein that are synthesized by bacteria. We think that after the production of LALF, the protein can pass to biofilm with the help of signal peptides we added. As long as LALF remains in the biofilm, the surface that is covered with that biofilm material will not be infected by gram negatives.</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/c/c9/Gjhjg%C3%B6hk%C3%A7j..png" alt="Gjhjgöhkçj..png (592×956)" width="274" height="442"/> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/1/1b/Ppk%C4%B1jougj.png" alt="Ppkıjougj.png (393×369)"/> <br /><br />
The LPS coat of gram negative bacteria is an important reason of endotoxin and septic shock. LALF binds to that LPS coat and inhibits the growth of bacteria. This ability is very crucial and may be very useful considering the diseases and pandemics that are caused by gram negatives. Our project suggests a possible prevention for such circumstances.<br /><br />
Also, by killing spores of B.subtilis on the biofilm surface with aqueous dissolved oxygen, ascorbic acid, and copper ions, we will try to perform a sterilized coating material which will possess a protective feature against infectious gram negative bacteria. </p><br />
<br />
<br />
<h3>REFERENCES</h3><br />
<ol><br />
<li>Alpert G, Baldwin G, Thompson C, Wainwright N, Novitsky T J, Gillis , Parsonnet J, Fleisher G R, Siber G R. Limulus antilipopolysaccharide<br />
factor protects rabbits from meningococcal endotoxin shock. J Infect Dis. 1992;165:494–500. [PubMed]</li><br />
<li>Bannerman D D, Goldblum S E. Endotoxin induces endothelial barrier dysfunction through protein tyrosine phosphorylation. Am J Physiol. 1997;273:L217–L226. [PubMed]<br />
</li><br />
<li>Battafaraono R J, Dahlberg P S, Ratz C A, Johnston J W, Gray B H, Haseman J R, Mayo K H, Dunn D L. Peptide derivatives of three distinct<br />
lipopolysaccharide binding proteins inhibit lipopolysaccharide-induced tumor necrosis factor-alpha secretion in vitro. Surgery. 1995;118:318–324. [PubMed]</li><br />
<li>Cooperstock M S. Inactivation of endotoxin by polymyxin B. Antimicrob Agents Chemother. 1974;6:422–425. [PMC free article] [PubMed]</li><br />
<li>Evans T J, Carpenter A, Moyes D, Martin R, Cohen J. Protective effects of a recombinant amino-terminal fragment of human<br />
bactericidal/permeability-increasing protein in an animal model of gram negative sepsis. J Infect Dis. 1995;171:153–160. [PubMed]</li><br />
<li>Fletcher M A, Mckena T M, Quance J L, Wainwright N R, Williams T J. Lipopolysaccharide detoxification by endotoxin neutralizing protein. J<br />
Surg Res. 1993;55:147–154. [PubMed]</li><br />
<li>Frey E A, Miller D S, Jahr T G, Sundan A, Bazil V, Espevik T, Finlay B B, Wright S D. Soluble CD14 participates in the response of cells to<br />
lipopolysaccharide. J Exp Med. 1992;176:1665–1671. [PMC free article] [PubMed]</li><br />
<li>Goldblum S E, Brann T W, Ding X, Pugin J, Tobias P S. Lipopolysaccharide (LPS)-binding protein and soluble CD14 function as accessory molecules for LPS-induced changes in endothelial barrier function, in vitro. J Clin Invest. 1994;93:692–702. [PMC free article] [PubMed]</li><br />
<li>Goldblum S E, Ding X, Brann T W, Campbell-Washington J. Bacterial lipopolysaccharide induces actin reorganization, intercellular gap<br />
formation, and endothelial barrier dysfunction in pulmonary vascular endothelial cells: concurrent F-actin depolymerization and new actin<br />
synthesis. J Cell Physiol. 1993;157:13–23. [PubMed]</li><br />
<li>Hirata M, Zhong J, Wright S C, Larrick J W. Structure and functions of endotoxin-binding peptides derived from CAP18. Prog Clin Biol Res.<br />
1995;392:317–326. [PubMed]</li><br />
<li>Hoess A, Watson S, Siber G R, Liddington R. Crystal structure of endotoxin-neutralizing protein from the horseshoe crab, Limulus anti-LPS<br />
factor, at 1.5 A resolution. EMBO J. 1993;12:3351–3356. [PMC free article] [PubMed]</li><br />
<li>Levine D M, Parker T S, Donelly T M, Walsh A, Rubin A L. In vivo protection against endotoxin by plasma high density lipoprotein. Proc Natl<br />
Acad Sci USA. 1993;90:12040–12044. [PMC free article] [PubMed]</li><br />
<li>Morita T, Ohtsubo S, Nakamura T, Tanaka S, Iwanaga S, Ohashi K, Niwa M. Isolation and biological activities of limulus anticoagulant<br />
(anti-LPS factor) which interacts with lipopolysaccharide (LPS) J Biochem (Tokyo) 1985;97:1611–1620. [PubMed]</li><br />
<li>Morrison D C, Jacobs D M. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry.<br />
1976;13:813–818. [PubMed]</li><br />
<li>Muta T, Miyata T, Tokunaga F, Nakamura T, Iwanaga S. Primary structure of anti-lipopolysaccharide factor from American horseshoe crab,<br />
Limulus polyphemus. J Biochem (Tokyo) 1987;101:1321–1330. [PubMed]</li><br />
<li>Netea M G, Demacker P N M, Kullberg B J, Boerman O C, Verschueren I, Stalenhoef A F H, van der Meer J W M. Low-density lipoprotein<br />
receptor-deficient mice are protected against lethal endotoxemia and severe Gram-negative infections. J Clin Invest. 1996;97:1366–1372.<br />
[PMC free article] [PubMed]</li><br />
<li>Ulevitch R J, Tobias P S. Recognition of endotoxin by cells leading to transmembrane signaling. Curr Opin Immunol. 1994;6:125–130.<br />
[PubMed]<br />
</li><br />
<li>Wainwright N R, Miller R J, Paus E, Novitsky T J, Fletcher M A, McKenna T M, Williams T. Endotoxin binding and neutralizing activity by a<br />
protein from Limulus polyphemus. In: Levin J, Alving C R, Munford R S, Stutz P L, editors. Cellular and molecular aspects of endotoxin<br />
reactions. New York, N.Y: Elsevier Science Publishers; 1990. pp. 315–325.</li><br />
<li>Warren H S, Novitsky T J, Bucklin A, Kania S A, Siber G R. Endotoxin neutralization with rabbit antisera to Escherichia coli J5 and other<br />
gram-negative bacteria. Infect Immun. 1987;55:1668–1673. [PMC free article] [PubMed]</li><br />
<li>Weinstein S L, June C H, DeFranco A L. Lipopolysaccharide-induced protein tyrosine phosphorylation in human macrophages is mediated<br />
by CD14. J Immunol. 1993;151:3829–3838. [PubMed]</li><br />
<li>A. Hoess, S. Watson, G.R.Siber and R.Liddington.Crystal structure of an endotoxin-neutralizing protein from the horseshoe crab, Limulus anti-LPS factor, at 1.5 A resolution.</li><br />
<li>Lihua Wu, Chao-Ming Tsai and Carl E. Frasch.A method purification of bacterial R-type LPS.</li><br />
<li>Kloczewiak, M., Black, K. M., Loiselle, P., Cavaillon, J. M., Wainwright, N., and Warren, H. S. (1994) J. Infect. Dis. 170, 1490–1497</li><br />
<li>http://www.horseshoecrab.org</li><br />
</ol><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/LALF
Team:Fatih Turkey/LALF
2011-10-29T00:46:35Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">LALF (Anti-LPS)</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"><br />
<br />
<br />
<p>As we know, seawater is a virtual &quot;bacterial soup&quot;. Typical near-shore areas that form the prime habitat of the horseshoe crab can easily contain over one billion Gram-negative bacteria per milliliter of seawater. Thus, the horseshoe crab is constantly threatened with infection. Unlike mammals, including humans, the horseshoe crab lacks an immune system; it cannot develop antibodies to fight infection. However, the horseshoe crab does contain a number of compounds that will bind to and inactivate bacteria, fungi, and viruses. Anti-LPS factors that are synthesized in the blood cells of crab are part of this primitive &quot;immune&quot; system.<br /><br />
Lots of horseshoe crabs are collected by some manufacture companies and their blood is extracted in some laboratories. During the extraction process, up to 30% of the animal's blood is removed. Research has shown that once returned to the water, the horseshoe crab's blood volume rebounds in about a week. But, it is also noted that a number of crabs which cannot be undervalued are also dying during the process.<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/2/2d/590_crash_blood.png" alt="590_crash_blood.png (613×359)"/> <br /><br />
</p><br />
<p>In our project, we aim to produce those factors by using the synthetic biology. In this way, we can obtain them without extracting blood and hurting any crab. Additionally, the possible medication will be gathered cheaper.<br /><br />
In our project, mainly we study on a protein that is gathered from horseshoe crab (<em>limulus polyphemus), </em>limulus anti lipopolysaccharide factor (LALF). By the help of this protein, we planned to stop bacteria growth in vitro situations. <br /><br />
Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge-shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock.<br /><br />
To make it possible, we use Bacillus Subtilis as a host; because it is supposed that this bacterium cannot be affected by LALF. Preventing such possibilities during our experiments will help us to get the best and the clearest results. <br /><br />
On the other hand, we want to apply our anti-LPS factor on a surface as a coat in order to obtain an anti-gram negative bacterial surface. Normally, B.subtilis has the ability to produce biofilm. This complex media may also include some components and protein that are synthesized by bacteria. We think that after the production of LALF, the protein can pass to biofilm with the help of signal peptides we added. As long as LALF remains in the biofilm, the surface that is covered with that biofilm material will not be infected by gram negatives.</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/c/c9/Gjhjg%C3%B6hk%C3%A7j..png" alt="Gjhjgöhkçj..png (592×956)" width="274" height="442"/> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/1/1b/Ppk%C4%B1jougj.png" alt="Ppkıjougj.png (393×369)"/> <br /><br />
The LPS coat of gram negative bacteria is an important reason of endotoxin and septic shock. LALF binds to that LPS coat and inhibits the growth of bacteria. This ability is very crucial and may be very useful considering the diseases and pandemics that are caused by gram negatives. Our project suggests a possible prevention for such circumstances.<br /><br />
Also, by killing spores of B.subtilis on the biofilm surface with aqueous dissolved oxygen, ascorbic acid, and copper ions, we will try to perform a sterilized coating material which will possess a protective feature against infectious gram negative bacteria. </p><br />
<br />
<br />
<h3>REFERENCES</h3><br />
<ol><br />
<li>Alpert G, Baldwin G, Thompson C, Wainwright N, Novitsky T J, Gillis , Parsonnet J, Fleisher G R, Siber G R. Limulus antilipopolysaccharide<br />
factor protects rabbits from meningococcal endotoxin shock. J Infect Dis. 1992;165:494–500. [PubMed]</li><br />
<li>Bannerman D D, Goldblum S E. Endotoxin induces endothelial barrier dysfunction through protein tyrosine phosphorylation. Am J Physiol. 1997;273:L217–L226. [PubMed]<br />
</li><br />
<li>Battafaraono R J, Dahlberg P S, Ratz C A, Johnston J W, Gray B H, Haseman J R, Mayo K H, Dunn D L. Peptide derivatives of three distinct<br />
lipopolysaccharide binding proteins inhibit lipopolysaccharide-induced tumor necrosis factor-alpha secretion in vitro. Surgery. 1995;118:318–324. [PubMed]</li><br />
<li>Cooperstock M S. Inactivation of endotoxin by polymyxin B. Antimicrob Agents Chemother. 1974;6:422–425. [PMC free article] [PubMed]</li><br />
<li>Evans T J, Carpenter A, Moyes D, Martin R, Cohen J. Protective effects of a recombinant amino-terminal fragment of human<br />
bactericidal/permeability-increasing protein in an animal model of gram negative sepsis. J Infect Dis. 1995;171:153–160. [PubMed]</li><br />
<li>Fletcher M A, Mckena T M, Quance J L, Wainwright N R, Williams T J. Lipopolysaccharide detoxification by endotoxin neutralizing protein. J<br />
Surg Res. 1993;55:147–154. [PubMed]</li><br />
<li>Frey E A, Miller D S, Jahr T G, Sundan A, Bazil V, Espevik T, Finlay B B, Wright S D. Soluble CD14 participates in the response of cells to<br />
lipopolysaccharide. J Exp Med. 1992;176:1665–1671. [PMC free article] [PubMed]</li><br />
<li>Goldblum S E, Brann T W, Ding X, Pugin J, Tobias P S. Lipopolysaccharide (LPS)-binding protein and soluble CD14 function as accessory molecules for LPS-induced changes in endothelial barrier function, in vitro. J Clin Invest. 1994;93:692–702. [PMC free article] [PubMed]</li><br />
<li>Goldblum S E, Ding X, Brann T W, Campbell-Washington J. Bacterial lipopolysaccharide induces actin reorganization, intercellular gap<br />
formation, and endothelial barrier dysfunction in pulmonary vascular endothelial cells: concurrent F-actin depolymerization and new actin<br />
synthesis. J Cell Physiol. 1993;157:13–23. [PubMed]</li><br />
<li>Hirata M, Zhong J, Wright S C, Larrick J W. Structure and functions of endotoxin-binding peptides derived from CAP18. Prog Clin Biol Res.<br />
1995;392:317–326. [PubMed]</li><br />
<li>Hoess A, Watson S, Siber G R, Liddington R. Crystal structure of endotoxin-neutralizing protein from the horseshoe crab, Limulus anti-LPS<br />
factor, at 1.5 A resolution. EMBO J. 1993;12:3351–3356. [PMC free article] [PubMed]</li><br />
<li>Levine D M, Parker T S, Donelly T M, Walsh A, Rubin A L. In vivo protection against endotoxin by plasma high density lipoprotein. Proc Natl<br />
Acad Sci USA. 1993;90:12040–12044. [PMC free article] [PubMed]</li><br />
<li>Morita T, Ohtsubo S, Nakamura T, Tanaka S, Iwanaga S, Ohashi K, Niwa M. Isolation and biological activities of limulus anticoagulant<br />
(anti-LPS factor) which interacts with lipopolysaccharide (LPS) J Biochem (Tokyo) 1985;97:1611–1620. [PubMed]</li><br />
<li>Morrison D C, Jacobs D M. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry.<br />
1976;13:813–818. [PubMed]</li><br />
<li>Muta T, Miyata T, Tokunaga F, Nakamura T, Iwanaga S. Primary structure of anti-lipopolysaccharide factor from American horseshoe crab,<br />
Limulus polyphemus. J Biochem (Tokyo) 1987;101:1321–1330. [PubMed]</li><br />
<li>Netea M G, Demacker P N M, Kullberg B J, Boerman O C, Verschueren I, Stalenhoef A F H, van der Meer J W M. Low-density lipoprotein<br />
receptor-deficient mice are protected against lethal endotoxemia and severe Gram-negative infections. J Clin Invest. 1996;97:1366–1372.<br />
[PMC free article] [PubMed]</li><br />
<li>Ulevitch R J, Tobias P S. Recognition of endotoxin by cells leading to transmembrane signaling. Curr Opin Immunol. 1994;6:125–130.<br />
[PubMed]<br />
</li><br />
<li>Wainwright N R, Miller R J, Paus E, Novitsky T J, Fletcher M A, McKenna T M, Williams T. Endotoxin binding and neutralizing activity by a<br />
protein from Limulus polyphemus. In: Levin J, Alving C R, Munford R S, Stutz P L, editors. Cellular and molecular aspects of endotoxin<br />
reactions. New York, N.Y: Elsevier Science Publishers; 1990. pp. 315–325.</li><br />
<li>Warren H S, Novitsky T J, Bucklin A, Kania S A, Siber G R. Endotoxin neutralization with rabbit antisera to Escherichia coli J5 and other<br />
gram-negative bacteria. Infect Immun. 1987;55:1668–1673. [PMC free article] [PubMed]</li><br />
<li>Weinstein S L, June C H, DeFranco A L. Lipopolysaccharide-induced protein tyrosine phosphorylation in human macrophages is mediated<br />
by CD14. J Immunol. 1993;151:3829–3838. [PubMed]</li><br />
<li>A. Hoess, S. Watson, G.R.Siber and R.Liddington.Crystal structure of an endotoxin-neutralizing protein from the horseshoe crab, Limulus anti-LPS factor, at 1.5 A resolution.</li><br />
<li>Lihua Wu, Chao-Ming Tsai and Carl E. Frasch.A method purification of bacterial R-type LPS.</li><br />
<li>Kloczewiak, M., Black, K. M., Loiselle, P., Cavaillon, J. M., Wainwright, N., and Warren, H. S. (1994) J. Infect. Dis. 170, 1490–1497</li><br />
<li>http://www.horseshoecrab.org</li><br />
</ol><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/LALF
Team:Fatih Turkey/LALF
2011-10-29T00:45:51Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">LALF (Anti-LPS)</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"><br />
<br />
<br />
<p>As we know, seawater is a virtual &quot;bacterial soup&quot;. Typical near-shore areas that form the prime habitat of the horseshoe crab can easily contain over one billion Gram-negative bacteria per milliliter of seawater. Thus, the horseshoe crab is constantly threatened with infection. Unlike mammals, including humans, the horseshoe crab lacks an immune system; it cannot develop antibodies to fight infection. However, the horseshoe crab does contain a number of compounds that will bind to and inactivate bacteria, fungi, and viruses. Anti-LPS factors that are synthesized in the blood cells of crab are part of this primitive &quot;immune&quot; system.<br /><br />
Lots of horseshoe crabs are collected by some manufacture companies and their blood is extracted in some laboratories. During the extraction process, up to 30% of the animal's blood is removed. Research has shown that once returned to the water, the horseshoe crab's blood volume rebounds in about a week. But, it is also noted that a number of crabs which cannot be undervalued are also dying during the process.<br /><br />
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/2/2d/590_crash_blood.png" alt="590_crash_blood.png (613×359)"/> <br /><br />
</p><br />
<p>In our project, we aim to produce those factors by using the synthetic biology. In this way, we can obtain them without extracting blood and hurting any crab. Additionally, the possible medication will be gathered cheaper.<br /><br />
In our project, mainly we study on a protein that is gathered from horseshoe crab (<em>limulus polyphemus), </em>limulus anti lipopolysaccharide factor (LALF). By the help of this protein, we planned to stop bacteria growth in vitro situations. <br /><br />
Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge-shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock.<br /><br />
To make it possible, we use Bacillus Subtilis as a host; because it is supposed that this bacterium cannot be affected by LALF. Preventing such possibilities during our experiments will help us to get the best and the clearest results. <br /><br />
On the other hand, we want to apply our anti-LPS factor on a surface as a coat in order to obtain an anti-gram negative bacterial surface. Normally, B.subtilis has the ability to produce biofilm. This complex media may also include some components and protein that are synthesized by bacteria. We think that after the production of LALF, the protein can pass to biofilm with the help of signal peptides we added. As long as LALF remains in the biofilm, the surface that is covered with that biofilm material will not be infected by gram negatives.</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/c/c9/Gjhjg%C3%B6hk%C3%A7j..png" alt="Gjhjgöhkçj..png (592×956)" width="274" height="442"/> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/1/1b/Ppk%C4%B1jougj.png" alt="Ppkıjougj.png (393×369)"/> <br /><br />
The LPS coat of gram negative bacteria is an important reason of endotoxin and septic shock. LALF binds to that LPS coat and inhibits the growth of bacteria. This ability is very crucial and may be very useful considering the diseases and pandemics that are caused by gram negatives. Our project suggests a possible prevention for such circumstances.<br /><br />
Also, by killing spores of B.subtilis on the biofilm surface with aqueous dissolved oxygen, ascorbic acid, and copper ions, we will try to perform a sterilized coating material which will possess a protective feature against infectious gram negative bacteria. </p><br />
<br />
<br />
<h3>REFERENCES</h3><br />
<ol><br />
<li>Alpert G, Baldwin G, Thompson C, Wainwright N, Novitsky T J, Gillis , Parsonnet J, Fleisher G R, Siber G R. Limulus antilipopolysaccharide<br />
factor protects rabbits from meningococcal endotoxin shock. J Infect Dis. 1992;165:494–500. [PubMed]</li><br />
<li>Bannerman D D, Goldblum S E. Endotoxin induces endothelial barrier dysfunction through protein tyrosine phosphorylation. Am J Physiol. 1997;273:L217–L226. [PubMed]<br />
</li><br />
<li>Battafaraono R J, Dahlberg P S, Ratz C A, Johnston J W, Gray B H, Haseman J R, Mayo K H, Dunn D L. Peptide derivatives of three distinct<br />
lipopolysaccharide binding proteins inhibit lipopolysaccharide-induced tumor necrosis factor-alpha secretion in vitro. Surgery. 1995;118:318–324. [PubMed]</li><br />
<li>Cooperstock M S. Inactivation of endotoxin by polymyxin B. Antimicrob Agents Chemother. 1974;6:422–425. [PMC free article] [PubMed]</li><br />
<li>Evans T J, Carpenter A, Moyes D, Martin R, Cohen J. Protective effects of a recombinant amino-terminal fragment of human<br />
bactericidal/permeability-increasing protein in an animal model of gram negative sepsis. J Infect Dis. 1995;171:153–160. [PubMed]</li><br />
<li>Fletcher M A, Mckena T M, Quance J L, Wainwright N R, Williams T J. Lipopolysaccharide detoxification by endotoxin neutralizing protein. J<br />
Surg Res. 1993;55:147–154. [PubMed]</li><br />
<li>Frey E A, Miller D S, Jahr T G, Sundan A, Bazil V, Espevik T, Finlay B B, Wright S D. Soluble CD14 participates in the response of cells to<br />
lipopolysaccharide. J Exp Med. 1992;176:1665–1671. [PMC free article] [PubMed]</li><br />
<li>Goldblum S E, Brann T W, Ding X, Pugin J, Tobias P S. Lipopolysaccharide (LPS)-binding protein and soluble CD14 function as accessory molecules for LPS-induced changes in endothelial barrier function, in vitro. J Clin Invest. 1994;93:692–702. [PMC free article] [PubMed]</li><br />
<li>Goldblum S E, Ding X, Brann T W, Campbell-Washington J. Bacterial lipopolysaccharide induces actin reorganization, intercellular gap<br />
formation, and endothelial barrier dysfunction in pulmonary vascular endothelial cells: concurrent F-actin depolymerization and new actin<br />
synthesis. J Cell Physiol. 1993;157:13–23. [PubMed]</li><br />
<li>Hirata M, Zhong J, Wright S C, Larrick J W. Structure and functions of endotoxin-binding peptides derived from CAP18. Prog Clin Biol Res.<br />
1995;392:317–326. [PubMed]</li><br />
<li>Hoess A, Watson S, Siber G R, Liddington R. Crystal structure of endotoxin-neutralizing protein from the horseshoe crab, Limulus anti-LPS<br />
factor, at 1.5 A resolution. EMBO J. 1993;12:3351–3356. [PMC free article] [PubMed]</li><br />
<li>Levine D M, Parker T S, Donelly T M, Walsh A, Rubin A L. In vivo protection against endotoxin by plasma high density lipoprotein. Proc Natl<br />
Acad Sci USA. 1993;90:12040–12044. [PMC free article] [PubMed]</li><br />
<li>Morita T, Ohtsubo S, Nakamura T, Tanaka S, Iwanaga S, Ohashi K, Niwa M. Isolation and biological activities of limulus anticoagulant<br />
(anti-LPS factor) which interacts with lipopolysaccharide (LPS) J Biochem (Tokyo) 1985;97:1611–1620. [PubMed]</li><br />
<li>Morrison D C, Jacobs D M. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry.<br />
1976;13:813–818. [PubMed]</li><br />
<li>Muta T, Miyata T, Tokunaga F, Nakamura T, Iwanaga S. Primary structure of anti-lipopolysaccharide factor from American horseshoe crab,<br />
Limulus polyphemus. J Biochem (Tokyo) 1987;101:1321–1330. [PubMed]</li><br />
<li>Netea M G, Demacker P N M, Kullberg B J, Boerman O C, Verschueren I, Stalenhoef A F H, van der Meer J W M. Low-density lipoprotein<br />
receptor-deficient mice are protected against lethal endotoxemia and severe Gram-negative infections. J Clin Invest. 1996;97:1366–1372.<br />
[PMC free article] [PubMed]</li><br />
<li>Ulevitch R J, Tobias P S. Recognition of endotoxin by cells leading to transmembrane signaling. Curr Opin Immunol. 1994;6:125–130.<br />
[PubMed]<br />
</li><br />
<li>Wainwright N R, Miller R J, Paus E, Novitsky T J, Fletcher M A, McKenna T M, Williams T. Endotoxin binding and neutralizing activity by a<br />
protein from Limulus polyphemus. In: Levin J, Alving C R, Munford R S, Stutz P L, editors. Cellular and molecular aspects of endotoxin<br />
reactions. New York, N.Y: Elsevier Science Publishers; 1990. pp. 315–325.</li><br />
<li>Warren H S, Novitsky T J, Bucklin A, Kania S A, Siber G R. Endotoxin neutralization with rabbit antisera to Escherichia coli J5 and other<br />
gram-negative bacteria. Infect Immun. 1987;55:1668–1673. [PMC free article] [PubMed]</li><br />
<li>Weinstein S L, June C H, DeFranco A L. Lipopolysaccharide-induced protein tyrosine phosphorylation in human macrophages is mediated<br />
by CD14. J Immunol. 1993;151:3829–3838. [PubMed]</li><br />
<li>A. Hoess, S. Watson, G.R.Siber and R.Liddington.Crystal structure of an endotoxin-neutralizing protein from the horseshoe crab, Limulus anti-LPS factor, at 1.5 A resolution.</li><br />
<li>Lihua Wu, Chao-Ming Tsai and Carl E. Frasch.A method purification of bacterial R-type LPS.</li><br />
<li>Kloczewiak, M., Black, K. M., Loiselle, P., Cavaillon, J. M., Wainwright, N., and Warren, H. S. (1994) J. Infect. Dis. 170, 1490–1497</li><br />
<li>http://www.horseshoecrab.org</li><br />
</ol><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Reflectin
Team:Fatih Turkey/Reflectin
2011-10-29T00:29:52Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Reflectin</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<p><strong>Reflectin</strong><br /><br />
Many cephalopods (squid, cuttlefish, octopus etc.) exhibit remarkable dermal iridescence (more than meets the eye), a component of their complex, dynamic camouflage and communication. In the species Euprymna scolopes, the light organ iridescence is static and is due to reflectin protein-based platelets assembled into lamellar thin-film reflectors called iridosomes, contained within iridescent cells called iridocytes.<br /><br />
Reflectin hierarchical protein assembly is necessity for the responsive, tunable optical function of iridosome cells. This protein assembly can be triggered by chemical stimulation and that assembly can be reversible and fine-tuned.<br /><br />
Namely reflectin proteins were identified as the major biomaterial component of iridosomes. The RA1 gene in The Rainbow Graveyard Project was artificially synthesized into pBluescript to transform into E. coli. In addition, Kramer et al. (2007) isolated reflectin proteins exhibit unusual solubility and self-association properties.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/2/20/Reflectinhkjljk..png" alt="Reflectinhkjljk..png (458×274)"/> <br /><br />
Self-association of RA1 is likely to stem from a combination of electrostatic and weak aromatic interactions. Reflectins are, indeed, characterized by their high content of polar aromatic residues and arginines, and several lines of evidence suggest that they are intrinsically unstructured, with no likely transmembrane, alphahelix or beta-sheet regions. E. scolopes reflectins also have high arginine, hydrophobic aromatic content and “methionine-rich membrane-associated proteins”. It is demonstrated that they have a high affinity for assembly with microsomal membranes.<br /><br />
The mechanism of reflectance is the same as that of colored soap bubbles. If the soap film (or multilayer plate) is very thin, shorter wavelengths are reflected, e.g. blue light; if it is thicker, longer wavelengths, such as yellow and red, are reflected. The reflectin proteins that apparently change their conformation or assembly to reversibly create the photonic structure.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/1/19/Reflectn1ujh%C4%B1s.png" alt="Reflectn1ujhıs.png (451×222)"/> </p><br />
<p>Multilayer reflector that appears red at near-normal viewing angles will appear first yellow, then green and blue at increasingly oblique angles. This dynamic reflection occurs by altering platelet and inter-platelet thicknesses in the multilayer reflector and/or altering the overall effective refractive index of the intra-platelet material. This allows the entire visible spectrum to be reflected from a single platelet stack. The increase in film thickness resulted in detectable redshift of the visible spectra and dominated any effect of decreasing refractive index owing to water sorption, which would have caused a blue-shift in the spectrum Kramer et al. (2007) have performed Micro-dialysis of reflectin 1a into various buffers, which resulted in two general types of aggregative structures. Optically clear bulk precipitation was seen in non-reducing conditions and filamentous protein structures were observed in reducing conditions controlled through the addition of a 10:1 ratio of reduced to oxidized glutathione .After several weeks at 4 ◦C, the filamentous structures formed a webbed structure that resulted in the supramolecular assembly of thin ribbons.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/5/50/Reflectnkjmld%C5%9Fkas.png" alt="Reflectnkjmldşkas.png (405×445)" width="429" height="469" align="middle"/><img src="https://static.igem.org/mediawiki/2011/a/a8/Squid_021005_42.jpg" alt="Squid_021005_42.jpg (500×386)" width="512" height="394" align="top"/><br /><br />
</p><br />
<p>In our project, we use reflectin 1A for detecting the survival of E.coli. Both of our bacteria (E.coli and B.subtilis) are expected to synthesize reflectin. As LALF is being produced by B.subtilis, both bacteria will synthesize reflectin. Meanwhile, LALF will bind to cell wall of gram negative bacterium (E.coli in our project). Therefore, gram (-) bacteria will not grow and survive in our biofilm surface. During the binding process, production of reflectin layer is going to decrease. Because of this, color of the surface will change due to the light reflecting ability of reflectin depending on the thickness of its layer. So, if the colour changes from red to blue, we can say there was a contact between E.coli and B.subtilis via anti-LPS factor.</p><br />
<br />
<br />
<h3>REFERENCES</h3><br />
<ol><br />
<li>nature materials VOL 6 JULY 2007<br />
(Review.The self-organizing properties of squid reflectin protein, Ryan M. Kramer et al.) <br />
Published online: 3 June 2007; doi:10.1038/nmat1930</li><br />
<li>J. R. Soc. Interface (2009) 6, S149–S163<br />
doi:10.1098/rsif.2008.0366.focus<br />
Published online 15 December 2008<br />
(Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S155)</li><br />
<li>Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S161</li><br />
<li>DOI: 10.1126/science.1091288<br />
Science 303, 235 (2004);<br />
Wendy J. Crookes, et al.(www.sciencemag.org SCIENCE VOL303 9 JANUARY 2004)</li><br />
<li>A.R. Tao et al. / Biomaterials 31 (2010) 793–801</li><br />
<li>J. R. Soc. Interface (2010) 7, 549–560 <br />
doi:10.1098/rsif.2009.0299<br />
Published online 23 September 2009(Reflectin phosphorylation M. Izumi et al.)</li><br />
</ol><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Reflectin
Team:Fatih Turkey/Reflectin
2011-10-29T00:16:15Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Project</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Reflectin</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<p><strong>Reflectin</strong><br /><br />
Many cephalopods (squid, cuttlefish, octopus etc.) exhibit remarkable dermal iridescence (more than meets the eye), a component of their complex, dynamic camouflage and communication. In the species Euprymna scolopes, the light organ iridescence is static and is due to reflectin protein-based platelets assembled into lamellar thin-film reflectors called iridosomes, contained within iridescent cells called iridocytes.<br /><br />
Reflectin hierarchical protein assembly is necessity for the responsive, tunable optical function of iridosome cells. This protein assembly can be triggered by chemical stimulation and that assembly can be reversible and fine-tuned.<br /><br />
Namely reflectin proteins were identified as the major biomaterial component of iridosomes. The RA1 gene in The Rainbow Graveyard Project was artificially synthesized into pBluescript to transform into E. coli. In addition, Kramer et al. (2007) isolated reflectin proteins exhibit unusual solubility and self-association properties.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/2/20/Reflectinhkjljk..png" alt="Reflectinhkjljk..png (458×274)"/> <br /><br />
Self-association of RA1 is likely to stem from a combination of electrostatic and weak aromatic interactions. Reflectins are, indeed, characterized by their high content of polar aromatic residues and arginines, and several lines of evidence suggest that they are intrinsically unstructured, with no likely transmembrane, alphahelix or beta-sheet regions. E. scolopes reflectins also have high arginine, hydrophobic aromatic content and “methionine-rich membrane-associated proteins”. It is demonstrated that they have a high affinity for assembly with microsomal membranes.<br /><br />
The mechanism of reflectance is the same as that of colored soap bubbles. If the soap film (or multilayer plate) is very thin, shorter wavelengths are reflected, e.g. blue light; if it is thicker, longer wavelengths, such as yellow and red, are reflected. The reflectin proteins that apparently change their conformation or assembly to reversibly create the photonic structure.</p><br />
<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/1/19/Reflectn1ujh%C4%B1s.png" alt="Reflectn1ujhıs.png (451×222)"/> </p><br />
<p>Multilayer reflector that appears red at near-normal viewing angles will appear first yellow, then green and blue at increasingly oblique angles. This dynamic reflection occurs by altering platelet and inter-platelet thicknesses in the multilayer reflector and/or altering the overall effective refractive index of the intra-platelet material. This allows the entire visible spectrum to be reflected from a single platelet stack. The increase in film thickness resulted in detectable redshift of the visible spectra and dominated any effect of decreasing refractive index owing to water sorption, which would have caused a blue-shift in the spectrum Kramer et al. (2007) have performed Micro-dialysis of reflectin 1a into various buffers, which resulted in two general types of aggregative structures. Optically clear bulk precipitation was seen in non-reducing conditions and filamentous protein structures were observed in reducing conditions controlled through the addition of a 10:1 ratio of reduced to oxidized glutathione .After several weeks at 4 ◦C, the filamentous structures formed a webbed structure that resulted in the supramolecular assembly of thin ribbons.</p><br />
<p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <img src="https://static.igem.org/mediawiki/2011/5/50/Reflectnkjmld%C5%9Fkas.png" alt="Reflectnkjmldşkas.png (405×445)"/> <br /><br />
In our project, we use reflectin 1A for detecting the survival of E.coli. Both of our bacteria (E.coli and B.subtilis) are expected to synthesize reflectin. As LALF is being produced by B.subtilis, both bacteria will synthesize reflectin. Meanwhile, LALF will bind to cell wall of gram negative bacterium (E.coli in our project). Therefore, gram (-) bacteria will not grow and survive in our biofilm surface. During the binding process, production of reflectin layer is going to decrease. Because of this, color of the surface will change due to the light reflecting ability of reflectin depending on the thickness of its layer. So, if the colour changes from red to blue, we can say there was a contact between E.coli and B.subtilis via anti-LPS factor.</p><br />
<br />
<h3>REFERENCES</h3><br />
<ol><br />
<li>nature materials VOL 6 JULY 2007<br />
(Review.The self-organizing properties of squid reflectin protein, Ryan M. Kramer et al.) <br />
Published online: 3 June 2007; doi:10.1038/nmat1930</li><br />
<li>J. R. Soc. Interface (2009) 6, S149–S163<br />
doi:10.1098/rsif.2008.0366.focus<br />
Published online 15 December 2008<br />
(Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S155)</li><br />
<li>Review. Structural coloration in cephalopods L. M. Ma¨thger et al. S161</li><br />
<li>DOI: 10.1126/science.1091288<br />
Science 303, 235 (2004);<br />
Wendy J. Crookes, et al.(www.sciencemag.org SCIENCE VOL303 9 JANUARY 2004)</li><br />
<li>A.R. Tao et al. / Biomaterials 31 (2010) 793–801</li><br />
<li>J. R. Soc. Interface (2010) 7, 549–560 <br />
doi:10.1098/rsif.2009.0299<br />
Published online 23 September 2009(Reflectin phosphorylation M. Izumi et al.)</li><br />
</ol><br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/Sporocide
Team:Fatih Turkey/Sporocide
2011-10-28T23:57:34Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<br />
<div class="body-2"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporiocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Sporicide</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"><br />
<!-- post container --><br />
<div><br />
<div class="meta-left-full"> <br />
<br />
<br />
<br />
<center> <b >EFFECTIVE SOLUTION TO BIG DANGER: SPORICIDE </b></center><br />
<p><em>Left: fenton reagent + aqueous dissolved oxygen<br /><br />
Right: fenton reagent + distilled water </em> <br /><br />
The reason that we chose B.subtilis in our project is the LPS binding character of LALF. Gram positive bacteria such as B.subtilis have no LPS layer on their cell wall. This obligation of choosing alternative bacterium leads to another problem: B.subtilis has the ability to form endospores. <br /><br />
B.subtilis, normally, has no pathogenic character unless it forms endospores under some circumstances like high temperature, limit pH levels or deoxygenized media. The inhalation of endospores is very dangerous and must be avoided in the case of study on B.subtilis.<br /><br />
Decontamination of biological endospores is very important considering the necessary of the safety in synthetic biology laboratories. Especially in iGEM, teams may need alternative creatures to plan and perform their projects properly like in our project. Because of this, we began to look through the web and hope to find a solution for this safety problem. Fortunately, some specific dilutions are characterized to solve possible safety problems in order to assemble aseptic surfaces. <strong>Fenton reagents</strong>, which kill septic microorganisms with mechanism of hydroxyl radicals, were traditionally being used. However; its major ingredient, hydrogen peroxide, was examined and was compared with aqueous dissolved oxygen about their capability of killing the spores. (J. B. Cross, 2003)1<br />
<br /><br />
It is said that in addition to aqueous dissolved oxygen, cupric chloride, ascorbic acid, sodium chloride and a little sulfactant dilution which are used as modified Fenton reagent are more effective on endospores of gram positive bacteria. In contrast; classic Fenton reagent, which possess hydrogen peroxide and copper ion, shows that gram negative bacteria are more vulnerable to this formulation. The mechanism of these dilutions is not well-characterized; however, comparing with other dilutions that include specific oxidizing agents such as ozone, some differences in the images that are gained from effected bacteria are indicated. It is deduced that modified Fenton reagent affects the inside mechanism of the bacteria; although the dilutions with ozone damages the coat of the bacteria. After formation of hydroxyl radicals within the spore, these highly reactive molecules attack either the DNA or enzymes necessary for the spore conversion to a bacterial cell. Further investigations of ion transport into spores and oxidation-reductions with in spores will be needed to determine precisely the mechanism of spore kill under these conditions.<br /><br />
The effectiveness of modified Fenton Reagent and the advantages/disadvantages of different Fenton Reagent solutions are characterized during our experiments. For more information about Fenton Reagent experiments, please visit <u>Fenton Reagent Experiments</u> page.<br /><br />
Another problem we faced was that the sporicide can also kill gram negative bacteria in their non-endospore forms. Our LALF protein is there for this reason; but we should be sure that gram negative bacteria are terminated just because of LALF protein. To achieve this, we decided to extract the oxygen ingradient which is the main component of killing mechanism in the Fenton reagent. Thus, hydroxyl radical mechanism of their agents would not work and only mechanism on the surface as terminator would be our anti-LPS factor against the gram negative bacteria, especially E.coli in our project.<br /><br />
Considering the importance of safe and secure labs in synthetic biology, especially in iGEM, prospective projects including B.subtilis may confront such problems. The need of sporicide will increase in the next iGEM competitions. To have safer labs, we recommend our chemical decontaminator to all iGEM teams.</p><br />
<p>&nbsp;</p><br />
<p>&nbsp;</p><br />
<p><strong>DISCUSSION</strong><br /><br />
As a conclusion, we observed <strong>distilled water</strong> included Fenton Reagent (DFR) is more effective than <strong>oxygenated water</strong> included Fenton Reagent (OFR) against E.coli. When 25 uL OFR and DFR have been added in E.coli cultures, 82% of DFR treated culture was dead and 47% of OFR treated culture was dead. We did not see any colony which included 50 uL and more volumes of DFR. In contrast, after 75 uL and more addition of OFR, no colony formation has been observed.</p><br />
<p>On the other hand, we observed OFR is more effective than DFR against Bacillus subtilis. When 10 uL OFR and DFR have been added in Bacillus subtilis cultures, 38% of DFR treated culture was dead and 98.3% of OFR treated culture was dead. Not only the 25 uL OFR treated but also 25 uL DFR treated culture was dead.</p><br />
<p>If we want to compare E.coli and B. subtilis, we can say that Fenton Reagent application is more fatal for Bacillus subtilis. Both 25 uL OFR and DFR treated Bacillus subtilis culture was dead but 25 uL OFR and DFR treated E.coli culture was still alive.</p><br />
<br />
<br><br />
<p>1 Killing of Bacillus Spores by Aqueous Dissolved Oxygen, Ascorbic Acid, and Copper Ions; J. B. Cross, R. P. Currier, D. J. Torraco, L. A. Vanderberg, G. L. Wagner, and P. D. Gladen Chemistry Division1 and Biosciences Division,2 Los Alamos National Laboratory, Los Alamos, New Mexico 87545 </p><br />
<br><br />
<br />
</div><br />
</div><br />
</div><br />
</div><br />
</div><br />
<br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet
http://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77
Team:Fatih Turkey/iGEM for 7 to 77
2011-10-28T23:53:55Z
<p>Fazilet: </p>
<hr />
<div><html xmlns="http://www.w3.org/1999/xhtml"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><br />
<title>deneme baslik</title><br />
<style><br />
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{<br />
display:none;<br />
}<br />
#menubar{<br />
background-color: transparent;<br />
font-size: 85%;<br />
top: 10px;<br />
position: absolute;<br />
margin-top: -12px;<br />
}<br />
<br />
div#content{<br />
padding: 0 !important;<br />
background: transparent;<br />
border: none !important;<br />
width: auto;<br />
}<br />
<br />
#menubar li a {<br />
color: #FFFFFF;<br />
background-color: transparent;<br />
}<br />
#globalWrapper{<br />
margin-top:5px;<br />
}<br />
#top-section{<br />
height: 20px !important;<br />
border: none !important;<br />
}<br />
body {<br />
font-size: 12px;<br />
font-family: Verdana, Arial, SunSans-Regular, Sans-Serif;<br />
color: #444; <br />
background: #6b6b6b url('https://static.igem.org/mediawiki/igem.org/a/ad/Bg-igem.png');<br />
}<br />
body,p,pre,div,span{<br />
line-height: 160%;<br />
color: #333;<br />
}<br />
html, body, div, span, object, iframe, h1, h2, h3, h4, h5, h6, blockquote, pre, a, abbr, acronym, address,<br />
code, del, dfn, em, img, q, dl, dt, dd, ul, li, fieldset, form, label, legend, table, caption, tbody, tfoot, thead, tr, th, td<br />
{<br />
margin:0;<br />
padding:0;<br />
border:0;<br />
font-weight:inherit;<br />
font-style:inherit;<br />
font-family:inherit;<br />
vertical-align:baseline;<br />
}<br />
<br />
em{<br />
font-style:italic;<br />
}<br />
table{<br />
background: transparent;<br />
margin-top: 15px;<br />
margin-bottom: 15px;<br />
}<br />
hr{<br />
border: 0;<br />
color: #eee;<br />
background-color: #eee;<br />
height: 1px;<br />
margin-bottom: 20px;<br />
}<br />
img.centered {<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
img.alignnone {<br />
margin: 0px 0px 5px 5px;<br />
display: inline;<br />
}<br />
img.alignright {<br />
margin: 0px 0px 5px 10px;<br />
display: inline;<br />
}<br />
<br />
img.alignleft {<br />
margin: 0px 10px 5px 0px;<br />
display: inline;<br />
}<br />
.alignright {<br />
float: right;<br />
}<br />
<br />
.alignleft {<br />
float: left;<br />
}<br />
.aligncenter{<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
h1,h2,h3,h4,h5,h6{<br />
margin-top: 15px;<br />
margin-bottom: 15px ;<br />
color: #333;<br />
line-height: 1.5em;<br />
}<br />
h1 {<br />
font-size: 30px;<br />
}<br />
h2 {<br />
font-size: 25px;<br />
}<br />
h3 {<br />
font-size: 20px;<br />
}<br />
h4 {<br />
font-size: 18px;<br />
}<br />
h5 {<br />
font-size: 16px;<br />
}<br />
<br />
h6 {<br />
font-size: 15px;<br />
}<br />
a{<br />
text-decoration: none;<br />
}<br />
a{<br />
color: #2f86c4;<br />
}<br />
a:hover{<br />
color: #4172ab;<br />
}<br />
#header .logo{<br />
margin-top: 41px;<br />
margin-left: 40px;<br />
}<br />
#footer{<br />
background: #707070; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid;<br />
width: 988px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
}<br />
#footer div{ <br />
color: #bbb;<br />
}<br />
#footer a{<br />
color: #c4c5cc;<br />
text-decoration: none;<br />
}<br />
#footer a:hover{<br />
color: #666;<br />
}<br />
<br />
.footer-sh-wrapper {<br />
height: 22px;<br />
margin: auto;<br />
position: relative;<br />
width: 990px;<br />
}<br />
.footer-sh-left {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b7/Footer-sh-left.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
left: 0;<br />
position: absolute;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
.footer-sh {<br />
background: url("https://static.igem.org/mediawiki/2011/b/bf/Footer-sh.png") repeat-x scroll center bottom #F9F9F9;<br />
bottom: 0;<br />
height: 22px;<br />
left: 10px;<br />
position: absolute;<br />
width: 970px;<br />
}<br />
.footer-sh-right {<br />
background: url("https://static.igem.org/mediawiki/2011/b/b5/Footer-sh-right.png") no-repeat scroll 0 0 transparent;<br />
height: 112px;<br />
position: absolute;<br />
right: 0;<br />
top: -90px;<br />
width: 10px;<br />
}<br />
<br />
.copyright-open{<br />
width: 988px;<br />
height: 0px;<br />
margin:auto;<br />
display: block-inline;<br />
}<br />
h1, h2, h3, h4, h5, h6{<br />
color: #333333;<br />
}<br />
#header-whole{<br />
<br />
position: relative;<br />
z-index: 200;<br />
width: 980px;<br />
margin: 0 auto;<br />
}<br />
#header{<br />
height: 100px;<br />
clear: both;<br />
background: #f9f9f9;<br />
margin: 0 auto;<br />
width: 970px;<br />
}<br />
#header .logo{<br />
float: left;<br />
margin-top: 15px;<br />
margin-left: 20px;<br />
}<br />
<br />
#container{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
background: none repeat scroll 0 0 #F9F9F9;<br />
}<br />
.copyright-open{<br />
border-top: 1px #a8a8a8 solid;<br />
border-bottom: 1px #888 solid; <br />
border-left: 1px #888 solid;<br />
border-right: 1px #888 solid; <br />
}<br />
.nav-wrapper{<br />
margin: 0px 90px 0px 0px;<br />
float:right;<br />
}<br />
.ddsmoothmenu ul{<br />
padding: 0px;<br />
list-style-type: none;<br />
background: #666666;<br />
}<br />
.ddsmoothmenu ul li{<br />
position: relative;<br />
display: block;<br />
float: left;<br />
}<br />
.ddsmoothmenu ul li a{<br />
line-height: 140%;<br />
overflow: hidden;<br />
text-align: left;<br />
font-family: 'Lucida Grande', Arial, Verdana, sans-serif;<br />
word-spacing: 1px;<br />
font-size: 14px;<br />
font-weight: normal;<br />
display: block; /*background of menu items (default state)*/<br />
padding: 34px 20px 10px 17px;<br />
text-decoration: none;<br />
}<br />
.ddsmoothmenu ul li a span {<br />
font-weight: normal;<br />
color: #888;<br />
font-size: 10px;<br />
clear: both;<br />
display:block;<br />
width: 60px;<br />
}<br />
* html .ddsmoothmenu ul li a{ /*IE6 hack to get sub menu links to behave correctly*/<br />
display: inline-block;<br />
}<br />
<br />
.ddsmoothmenu ul li a:link, .ddsmoothmenu ul li a:visited{<br />
color: #333;<br />
}<br />
<br />
.ddsmoothmenu ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #888;<br />
}<br />
<br />
.ddsmoothmenu ul li a:hover{<br />
color: #888;<br />
}<br />
<br />
/*1st sub level menu*/<br />
.ddsmoothmenu ul li ul{<br />
position: absolute;<br />
left: 0;<br />
visibility: hidden;<br />
display: none; /*collapse all sub menus to begin with*/<br />
border: 1px #222 solid;<br />
-webkit-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
-moz-box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
box-shadow: rgba(0, 0, 0, 0.2) 0px 0px 2px 1px;<br />
}<br />
<br />
/*Sub level menu list items (undo style from Top level List Items)*/<br />
.ddsmoothmenu ul li ul li{<br />
text-align:left;<br />
float: none;<br />
background: url('img/bg-menu2.png');<br />
}<br />
<br />
.ddsmoothmenu ul li ul li a{<br />
color: #fff;<br />
font-size: 12px;<br />
}<br />
.ddsmoothmenu ul li ul li a.selected{ /*CSS class that's dynamically added to the currently active menu items' LI A element*/<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:link, .ddsmoothmenu ul li ul li a:visited{<br />
color: #fff;<br />
}<br />
.ddsmoothmenu ul li ul li a:hover{<br />
color: #ffc;<br />
}<br />
.ddsmoothmenu ul li ul li ul{<br />
top: 0;<br />
}<br />
<br />
/* Sub level menu links style */<br />
.ddsmoothmenu ul li ul li a{<br />
width: 160px; /*width of sub menus*/<br />
padding: 0px 0px 0px 10px;<br />
height: 38px;<br />
line-height: 320%;<br />
border-left: 1px #555 solid;<br />
overflow: hidden;<br />
}<br />
<br />
/* Holly Hack for IE \*/<br />
* html .ddsmoothmenu{height: 1%;} /*Holly Hack for IE7 and below*/<br />
<br />
.copyright{<br />
width: 930px;<br />
color: #959595;<br />
background: #eee;<br />
clear: both;<br />
line-height: 190%;<br />
text-align: right;<br />
padding: 10px 20px 10px;<br />
margin: 0px auto 0px;<br />
}<br />
.copyright a{<br />
color: #bbb;<br />
text-decoration: none;<br />
}<br />
.copyright a:hover{<br />
color: #ccc;<br />
} <br />
.bar-title-whole{<br />
overflow: visible;<br />
position: relative;<br />
width: 990px;<br />
margin: auto;<br />
z-index: 10;<br />
}<br />
.bar-title{<br />
background: #999 url('https://static.igem.org/mediawiki/2011/e/e9/Bar-title-bg.jpg') top;<br />
border-left: 1px #8a8a8a solid;<br />
border-right: 1px #8a8a8a solid;<br />
<br />
width: 918px;<br />
height: 122px;<br />
margin: auto;<br />
overflow: hidden;<br />
padding-left: 35px;<br />
padding-right: 35px;<br />
color: #ddd;<br />
line-height: 150%;<br />
font-size: 12px;<br />
<br />
position: relative;<br />
z-index: 10;<br />
}<br />
<br />
.bar-title h2{<br />
color: #fff;<br />
margin-top: 15px;<br />
margin-bottom: 30px !important;<br />
}<br />
.bar-title h5{<br />
color: #eee;<br />
}<br />
.bar-title-sh-left{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 0px;<br />
}<br />
.bar-title-sh{<br />
width: 970px;<br />
height: 8px;<br />
background: url('https://static.igem.org/mediawiki/2011/3/37/Bar-title-sh.png') repeat-x;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
left: 10px;<br />
}<br />
.bar-title-sh-right{<br />
width: 10px;<br />
height: 112px;<br />
background: url('https://static.igem.org/mediawiki/2011/c/c6/Bar-title-sh-right.png') no-repeat;<br />
display: block-inline;<br />
position: absolute;<br />
top: 122px;<br />
right: 0px;<br />
}<br />
#page{<br />
width: 970px;<br />
margin: 0px auto 0px;<br />
overflow: hidden;<br />
background: #f9f9f9;<br />
<br />
}<br />
<br />
#page .page-wrapper{<br />
margin: 15px 30px 0px 30px;<br />
width: 910px;<br />
overflow: hidden;<br />
float: left;<br />
}<br />
#page .page-wrapper-right{<br />
margin: 30px 40px 0px 25px;<br />
width: 935px;<br />
overflow: hidden;<br />
float: right;<br />
}<br />
#page .title-wrapper h1{<br />
margin-top: 0.5em;<br />
margin-bottom: 0.3em;<br />
}<br />
#page .meta-left-full{<br />
margin: 15px 0px 5px 0px;<br />
overflow: hidden;<br />
} <br />
#page .meta-left-button{<br />
margin: 5px 0px 60px 0px;<br />
overflow: hidden;<br />
}<br />
#page .w600{<br />
width: 600px;<br />
}<br />
<br />
</style><br />
<br />
<br />
</head><br />
<body><br />
<script type="text/javascript" src="http://simsekburak.com/fatihigem/ddsmoothmenu.js"></script><br />
<br />
<script><br />
$(document).ready( <br />
ddsmoothmenu.init({<br />
mainmenuid: "topmenu",<br />
orientation: 'h',<br />
classname: 'ddsmoothmenu',<br />
contentsource: "markup"<br />
}));<br />
<br />
jQuery(window).load(function() {<br />
setTimeout(function(){<br />
jQuery('#slider').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNav:true,<br />
directionNavHide:true,<br />
controlNavThumbs:true,<br />
controlNavThumbsFromRel:true,<br />
});<br />
}, 1000),<br />
setTimeout(function(){<br />
/* For slider shortcode */<br />
jQuery('#slider2').nivoSlider({ <br />
pauseTime:4000,<br />
animSpeed:600,<br />
captionOpacity: 0.8,<br />
pauseOnHover:true,<br />
directionNavHide:true });<br />
}, 1000);<br />
<br />
});<br />
</script><br />
<div class="body-2" id="top"><br />
<!-- Header --><br />
<div id="header-whole"><br />
<div id="header"><br />
<a href="https://2011.igem.org/" style="position:absolute; right:10px; top:5px;"><img src="https://static.igem.org/mediawiki/2011/9/9c/Igem_basic_logo.png"/></a><br />
<!-- Logo --><br />
<div><br />
<div class="logo"><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey"><br />
<img width="258" src="https://static.igem.org/mediawiki/2011/b/b6/Fatih_turkey_logo.png"><br />
</a><br />
</div><br />
</div><br />
<!-- Menu --><br />
<div class="nav-wrapper"><br />
<div class="nav-main"><br />
<div id="topmenu" class="ddsmoothmenu"><br />
<ul id="menu-main" class="menu"><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey">Home</a><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Overall Project</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/LALF">LALF</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Reflectin">Reflectin</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Biofilm">Biofilm</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Experiments">Experiments</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Future_Plan">Future Plan</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a><br />
</li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Team">Team</a><br />
<ul><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Members">Members</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Gallery">Gallery</a></li><br />
<li><a <br />
href="https://2011.igem.org/Team:Fatih_Turkey/Europe_Jamboree">Europe Jamboree</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Human_Practice">Human Practice</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporicide</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/iGEM_for_7_to_77">iGEM for 7 to 77</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/game">Game</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/canvas_times">Canvas Times</a></li><br />
<li><a<br />
href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/collaboration">Collaboration</a></li><br />
</ul><br />
</li><br />
<li><br />
<a href="https://2011.igem.org/Team:Fatih_Turkey/Lab_Garage">Lab Garage</a><br />
<ul><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook">Notebook</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Procedures">Procedures</a></li><br />
</ul><br />
</li><br />
</ul><br />
</div><br />
</div><br />
</div><br />
</div> <br />
</div><br />
<!-- End Header --><br />
<div class="bar-title-whole"><br />
<div class="bar-title"><br />
<h2 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">Human Practice</h2><br />
<h5 style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;">iGEM for 7 to 77</h5> <br />
</div> <br />
<div class="bar-title-sh-left"></div><br />
<div class="bar-title-sh"></div><br />
<div class="bar-title-sh-right"></div><br />
</div><br />
</div><br />
<div id="container"><br />
<div id="page"><br />
<div class="page-wrapper"> <br />
<div style="position: relative;"><br />
<div style="float: left; width: 220px; border-radius: 10px; position: absolute; top: 50px; left: 0; background: #EEE; border: solid 3px #666;"><br />
<ol><br />
<br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_1">High School Divisions</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_2">Government for SynBio</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_3">Fatih Turkey in Media</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_4">Fatih Turkey in Kindergarten</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_5">Yasama Sanati (The Art of Living)</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_6">Survey</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_7">Visitors of Lab</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_8">Gifted Childiren</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_9">Opinions of Med Students</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_10">Medical Conference</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_11">Politics Club</a></li><br />
<li><a href="https://2011.igem.org/Team:Fatih_Turkey/for7to77_12">Synthetic Biology Meeting Turkey</a></li><br />
<br />
</ol><br />
</div><br />
<div style="width: 654px; border: solid 3px #666; border-radius: 10px; padding-left: 80px; margin-left: 170px;"><br />
<br />
<table width="594" height="203" border="0"><br />
<tr><br />
<td height="199" valign="top"><b>HIGH SCHOOL DIVISION</b><br /><br />
<p><strong><img src="https://static.igem.org/mediawiki/2011/1/1f/IMG_0549.JPG" height="195" /></strong><br />
<strong><img src="https://static.igem.org/mediawiki/2011/3/37/IMG_0554.JPG" height="195"/></strong></p><br />
<br />
<br />
<br />
<br />
<p><br />
High schools have started to join iGEM from this year. We think that this is really important and necessary. However, how much are the high schools in Turkey aware of iGEM and synthetic biology?<br /><br />
As Fatih_Turkey team, we went to some high schools of Ankara to inform high school students about that opportunity and also to help them to assemble their own teams. Firstly, we gave a presentation, told them the future of synthetic biology and its fields of use. Then, we introduced them with iGEM, told the rationale behind it, interesting projects from previous years, and our own project. Also, in order to teach iGEM and synthetic biology in an enjoyable way we played our game altogether. Students who played the game understood the iGEM and logic of biobrick more clearly. After the presentation and the game, we got very good reactions from students. They said that they would like to assemble a team and asked about the materials required for laboratory studies. We talked about the instruments and materials and also stated that we could help them whenever they needed.<br /><br />
As a result, we realized that high school students did not know much about synthetic biology and iGEM, which is quite natural. However, we believe that if we tell and encourage them, some students might choose to study in this field which may lead new useful projects. As Fatih Turkey team, we will support, encourage and help them as much as we can for next years’ competitions.</p><br />
<p>The high schools which we informed:<br /><br />
- Ahmet Ulusoy High School<br /><br />
- Private Samanyolu Serhat High School<br /><br />
- Nene Hatun High School<br /><br />
- Nevin Gökçek High School<br /><br />
- Öncü High School</p><br />
<p>In order to teach iGEM and synthetic biology in a funny way to people of all ages, we prepared a monopoly-like game. We played the game in our own university, and also in high schools where we went to introduce iGEM. We had a great time while playing the game with our friends and teenagers from the high schools. We also got really good reactions from the players, and one of the most interesting suggestions was that we had to sell this game.<br /><br />
Moreover, the players were given a survey prepared to measure the effect of the game. What is synthetic biology? What can be done by it? What is the logic behind the projects of iGEM? Those were the questions that they could find the answers in this game. The game not only gave them an idea about the iGEM or synthetic biology, but it also taught them the names of some mostly used antibiotics, principles of some experiments, and laboratory instruments, such as autoclave. <br /><br />
All the process is full of unforgettable moments, and they will remain in our memories for long and long years.</p></td><br />
</tr><br />
</table><br />
<br />
<p><strong><img src="https://static.igem.org/mediawiki/2011/7/70/2011-10-25_14.13.02.jpg" height="290" /></strong><br />
<br />
<strong><img src="https://static.igem.org/mediawiki/2011/7/72/IMG_0580.JPG" height="290" /></strong></p><br />
<br />
<br><br />
<br><br />
<br />
<br />
</div><br />
</div><br />
<br />
</div><br />
</div><br />
</div><br />
<div class="footer-sh-wrapper"><br />
<div class="footer-sh-left"></div><br />
<div class="footer-sh"></div><br />
<div class="footer-sh-right"></div><br />
</div><br />
<div id="footer"><br />
<style><br />
#footer img:hover{<br />
opacity:0.6;<br />
}<br />
</style><br />
<div><br />
<h3 style="color:#BBB;">Sponsors</h3><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/7/72/Tubitak_-_Kopya.png"/><br />
<a href="http://www.sentegen.com"><br />
<img height="100" src="https://static.igem.org/mediawiki/2011/2/2d/Sentegennnn.png"/></a><br />
</div><br />
</div><br />
<div class="copyright-open"> <br />
</div><br />
<div class="copyright"><br />
2011 © Fatih Medical School<br />
</div><br />
</div><br />
<br />
<br />
</body><br />
</html></div>
Fazilet