http://2011.igem.org/wiki/index.php?title=Solution_II&feed=atom&action=history
Solution II - Revision history
2024-03-29T10:21:17Z
Revision history for this page on the wiki
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http://2011.igem.org/wiki/index.php?title=Solution_II&diff=75332&oldid=prev
DrLiz at 02:39, 15 August 2011
2011-08-15T02:39:03Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:39, 15 August 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 2.50 ml 0.4 N NaOH</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 2.50 ml 0.4 N NaOH</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 0.05 ml 10% SDS</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 0.05 ml 10% SDS</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> 2.45 ml distilled water or NF water</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> 2.45 ml distilled water or <ins class="diffchange diffchange-inline">[[</ins>NF water<ins class="diffchange diffchange-inline">]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Notice 0.05 ml = 50 ul of the 10% SDS.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Notice 0.05 ml = 50 ul of the 10% SDS.</div></td></tr>
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DrLiz
http://2011.igem.org/wiki/index.php?title=Solution_II&diff=75331&oldid=prev
DrLiz at 02:38, 15 August 2011
2011-08-15T02:38:07Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 0.05 ml 10% SDS</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 0.05 ml 10% SDS</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 2.45 ml distilled water or NF water</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 2.45 ml distilled water or NF water</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Notice 0.05 ml = 50 ul of the 10% SDS.</ins></div></td></tr>
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DrLiz
http://2011.igem.org/wiki/index.php?title=Solution_II&diff=75330&oldid=prev
DrLiz: replace % with N
2011-08-15T02:36:41Z
<p>replace % with N</p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:36, 15 August 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The ideal Solution II would be freshly made, using 10 N NaOH.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The ideal Solution II would be freshly made, using 10 N NaOH.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To make 5 ml of Solution II using 0.4<del class="diffchange diffchange-inline">% </del>NaOH stock and 10% SDS stock solution we use:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To make 5 ml of Solution II using 0.4 <ins class="diffchange diffchange-inline">N </ins>NaOH stock and 10% SDS stock solution we use:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 2.50 ml 0.4 N NaOH</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 2.50 ml 0.4 N NaOH</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 0.05 ml 10% SDS</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 0.05 ml 10% SDS</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 2.45 ml distilled water or NF water</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 2.45 ml distilled water or NF water</div></td></tr>
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DrLiz
http://2011.igem.org/wiki/index.php?title=Solution_II&diff=75329&oldid=prev
DrLiz: correct % with substitution of N
2011-08-15T02:36:13Z
<p>correct % with substitution of N</p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:36, 15 August 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To make 5 ml of Solution II using 0.4% NaOH stock and 10% SDS stock solution we use:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To make 5 ml of Solution II using 0.4% NaOH stock and 10% SDS stock solution we use:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> 2.50 ml 0.4<del class="diffchange diffchange-inline">% </del>NaOH</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> 2.50 ml 0.4 <ins class="diffchange diffchange-inline">N </ins>NaOH</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 0.05 ml 10% SDS</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 0.05 ml 10% SDS</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 2.45 ml distilled water or NF water</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> 2.45 ml distilled water or NF water</div></td></tr>
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DrLiz
http://2011.igem.org/wiki/index.php?title=Solution_II&diff=75328&oldid=prev
DrLiz: Making Solution II for Plasmid Protein Purification
2011-08-15T02:35:29Z
<p>Making Solution II for Plasmid Protein Purification</p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:35, 15 August 2011</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>0.2 <del class="diffchange diffchange-inline">M </del>NaOH </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1% <del class="diffchange diffchange-inline"> </del>SDS</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>0.2 <ins class="diffchange diffchange-inline">N </ins>NaOH <ins class="diffchange diffchange-inline"> [Think M when you see N]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>1<ins class="diffchange diffchange-inline">.0</ins>% SDS</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The ideal Solution II would be freshly made, using 10 N NaOH.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">2.</del>5 <del class="diffchange diffchange-inline"> </del>ml of 0.4 <del class="diffchange diffchange-inline">M </del>NaOH</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">To make </ins>5 ml of <ins class="diffchange diffchange-inline">Solution II using </ins>0.4<ins class="diffchange diffchange-inline">% </ins>NaOH <ins class="diffchange diffchange-inline">stock and 10% SDS stock solution we use:</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>0.05 ml <del class="diffchange diffchange-inline">of </del>10% SDS </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> 2.50 ml 0.4% NaOH</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>2.45 ml <del class="diffchange diffchange-inline">Nuclease free Water</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>0.05 ml 10% SDS</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>2.45 ml <ins class="diffchange diffchange-inline">distilled water or NF water</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">lends 5 mls of solution</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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DrLiz
http://2011.igem.org/wiki/index.php?title=Solution_II&diff=57986&oldid=prev
Mduley at 21:18, 28 July 2011
2011-07-28T21:18:51Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 21:18, 28 July 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>0.2 M NaOH </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>0.2 M NaOH </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1% SDS</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1% SDS</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">2.5 ml of 0.4 M NaOH</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">0.05 ml of 10% SDS </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">2.45 ml Nuclease free Water</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">lends 5 mls of solution</ins></div></td></tr>
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Mduley
http://2011.igem.org/wiki/index.php?title=Solution_II&diff=57939&oldid=prev
Mduley: Created page with "0.2 M NaOH 1% SDS"
2011-07-28T20:48:33Z
<p>Created page with "0.2 M NaOH 1% SDS"</p>
<p><b>New page</b></p><div>0.2 M NaOH <br />
1% SDS</div>
Mduley