Solution II

From 2011.igem.org

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   2.50 ml 0.4 N NaOH
   2.50 ml 0.4 N NaOH
   0.05 ml 10% SDS
   0.05 ml 10% SDS
-
   2.45 ml distilled water or NF water
+
   2.45 ml distilled water or [[NF water]]
Notice 0.05 ml = 50 ul of the 10% SDS.
Notice 0.05 ml = 50 ul of the 10% SDS.

Latest revision as of 02:39, 15 August 2011

0.2 N NaOH    [Think M when you see N]
1.0% SDS

The ideal Solution II would be freshly made, using 10 N NaOH.

To make 5 ml of Solution II using 0.4 N NaOH stock and 10% SDS stock solution we use:

 2.50 ml 0.4 N NaOH
 0.05 ml 10% SDS
 2.45 ml distilled water or NF water

Notice 0.05 ml = 50 ul of the 10% SDS.