Solution II

From 2011.igem.org

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(Making Solution II for Plasmid Protein Purification)
 
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The ideal Solution II would be freshly made, using 10 N NaOH.
The ideal Solution II would be freshly made, using 10 N NaOH.
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To make 5 ml of Solution II using 0.4% NaOH stock and 10% SDS stock solution we use:
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To make 5 ml of Solution II using 0.4 N NaOH stock and 10% SDS stock solution we use:
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   2.50 ml 0.4% NaOH
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   2.50 ml 0.4 N NaOH
   0.05 ml 10% SDS
   0.05 ml 10% SDS
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   2.45 ml distilled water or NF water
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   2.45 ml distilled water or [[NF water]]
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Notice 0.05 ml = 50 ul of the 10% SDS.

Latest revision as of 02:39, 15 August 2011

0.2 N NaOH    [Think M when you see N]
1.0% SDS

The ideal Solution II would be freshly made, using 10 N NaOH.

To make 5 ml of Solution II using 0.4 N NaOH stock and 10% SDS stock solution we use:

 2.50 ml 0.4 N NaOH
 0.05 ml 10% SDS
 2.45 ml distilled water or NF water

Notice 0.05 ml = 50 ul of the 10% SDS.