"
Solution II
From 2011.igem.org
(Difference between revisions)
(replace % with N) |
m |
||
| Line 9: | Line 9: | ||
0.05 ml 10% SDS | 0.05 ml 10% SDS | ||
2.45 ml distilled water or NF water | 2.45 ml distilled water or NF water | ||
| + | |||
| + | Notice 0.05 ml = 50 ul of the 10% SDS. | ||
Revision as of 02:38, 15 August 2011
0.2 N NaOH [Think M when you see N] 1.0% SDS
The ideal Solution II would be freshly made, using 10 N NaOH.
To make 5 ml of Solution II using 0.4 N NaOH stock and 10% SDS stock solution we use:
2.50 ml 0.4 N NaOH 0.05 ml 10% SDS 2.45 ml distilled water or NF water
Notice 0.05 ml = 50 ul of the 10% SDS.
