Solution II

From 2011.igem.org

(Difference between revisions)
(Making Solution II for Plasmid Protein Purification)
m (correct % with substitution of N)
Line 6: Line 6:
To make 5 ml of Solution II using 0.4% NaOH stock and 10% SDS stock solution we use:
To make 5 ml of Solution II using 0.4% NaOH stock and 10% SDS stock solution we use:
-
   2.50 ml 0.4% NaOH
+
   2.50 ml 0.4 N NaOH
   0.05 ml 10% SDS
   0.05 ml 10% SDS
   2.45 ml distilled water or NF water
   2.45 ml distilled water or NF water

Revision as of 02:36, 15 August 2011

0.2 N NaOH    [Think M when you see N]
1.0% SDS

The ideal Solution II would be freshly made, using 10 N NaOH.

To make 5 ml of Solution II using 0.4% NaOH stock and 10% SDS stock solution we use:

 2.50 ml 0.4 N NaOH
 0.05 ml 10% SDS
 2.45 ml distilled water or NF water