Solution I


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50 mM glucose                  Link to 50 mM glucose
25 mM Tris Cl (pH 8.0)
10 mM EDTA (pH 8.0)

Solution I can be prepared in batches of approximately 100 ml, autoclaved for 15 minutes at 10lb/sq. in. on liquid cycle, and stored at 4 degrees C

This is 1 of 3 solutions that one can use in a process to purify plasmid DNA. The advantage of using Solutions I, II and III is that the reagents required to make these solutions are readily available - most of what was used to make these solutions was donated by colleagues who had the compounds in pure, powdered form. Because these same colleagues have money for things like "quick" spin columns for DNA purification, many of the ingredients were simply collecting dust in a cabinet.