Sensor: Week 8 July 5-8

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Tuesday, July 5

Clone OR and CRO

Protocol Part Involved with Protocol
Restriction Digest Inserts using XbaI and PstI: OR
CRO
Vector using XbaI and PstI: K3
Ligation K3 + OR
K3 + CRO
Transformation/Plating The above ligations were transformed into
Escherichia coli cells and plated onto
kanamycin resistant plates.

cI Repressor Test Assembly, Day 27

Sequencing Results: The following constructs were sent to sequencing to verify their DNA sequences:

Construct Sequencing
Result
J23113 + B0034 + C0051 Good
KOR + K648000 Good



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