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Sensor: Week 8 July 5-8
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(Created page with "==Tuesday, July 5== ===Clone O<sub>R</sub> and CRO=== {|border="1" !Protocol |colspan="2" align="center"|'''Part Involved with Protocol''' |- |rowspan="2"|Restriction Digest |Ins...")
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Revision as of 17:49, 13 July 2011
Tuesday, July 5
Clone OR and CRO
| Protocol | Part Involved with Protocol | |
|---|---|---|
| Restriction Digest | Inserts using XbaI and PstI: | OR CRO |
| Vector using XbaI and PstI: | K3 | |
| Ligation | K3 + OR K3 + CRO | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
cI Repressor Test Assembly, Day 27
Sequencing Results: The following constructs were sent to sequencing to verify their DNA sequences:
| Construct | Sequencing Result | J23113 + B0034 + C0051 | Good |
|---|---|---|---|
| KOR + K648000 | Good |
