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Sensor: Week 8 July 5-8
From 2011.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
|- | |- | ||
|Transformation/Plating | |Transformation/Plating | ||
| - | |colspan="2"|The above ligations were transformed into Escherichia<br />coli cells and plated onto kanamycin | + | |colspan="2"|The above ligations were transformed into Escherichia<br />coli cells and plated onto kanamycin resistant plates. |
|} | |} | ||
| Line 26: | Line 26: | ||
|- | |- | ||
|J23113 + B0034 + C0051 | |J23113 + B0034 + C0051 | ||
| - | |Good | + | |align="center"|Good |
|- | |- | ||
|KOR + K648000 | |KOR + K648000 | ||
| - | |Good | + | |align="center"|Good |
|} | |} | ||
Revision as of 17:51, 13 July 2011
Tuesday, July 5
Clone OR and CRO
| Protocol | Part Involved with Protocol | |
|---|---|---|
| Restriction Digest | Inserts using XbaI and PstI: | OR CRO |
| Vector using XbaI and PstI: | K3 | |
| Ligation | K3 + OR K3 + CRO | |
| Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto kanamycin resistant plates. | |
cI Repressor Test Assembly, Day 27
Sequencing Results: The following constructs were sent to sequencing to verify their DNA sequences:
| Construct | Sequencing Result |
|---|---|
| J23113 + B0034 + C0051 | Good |
| KOR + K648000 | Good |
