Sensor: Week 8 July 5-8

From 2011.igem.org

(Difference between revisions)
(Created page with "==Tuesday, July 5== ===Clone O<sub>R</sub> and CRO=== {|border="1" !Protocol |colspan="2" align="center"|'''Part Involved with Protocol''' |- |rowspan="2"|Restriction Digest |Ins...")
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|Transformation/Plating
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|colspan="2"|The above ligations were transformed into <br />Escherichia coli cells and plated onto kanamycin<br />resistant plates.
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|colspan="2"|The above ligations were transformed into Escherichia<br />coli cells and plated onto kanamycin<br />resistant plates.
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!Construct
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!Sequencing <br /> Result
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|J23113 + B0034 + C0051
|J23113 + B0034 + C0051
|Good
|Good

Revision as of 17:50, 13 July 2011

Tuesday, July 5

Clone OR and CRO

Protocol Part Involved with Protocol
Restriction Digest Inserts using XbaI and PstI: OR
CRO
Vector using XbaI and PstI: K3
Ligation K3 + OR
K3 + CRO
Transformation/Plating The above ligations were transformed into Escherichia
coli cells and plated onto kanamycin
resistant plates.

cI Repressor Test Assembly, Day 27

Sequencing Results: The following constructs were sent to sequencing to verify their DNA sequences:

Construct Sequencing
Result
J23113 + B0034 + C0051 Good
KOR + K648000 Good



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