Sensor: Week 8 July 5-8
From 2011.igem.org
(Difference between revisions)
(Created page with "==Tuesday, July 5== ===Clone O<sub>R</sub> and CRO=== {|border="1" !Protocol |colspan="2" align="center"|'''Part Involved with Protocol''' |- |rowspan="2"|Restriction Digest |Ins...") |
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- | |colspan="2"|The above ligations were transformed into <br /> | + | |colspan="2"|The above ligations were transformed into Escherichia<br />coli cells and plated onto kanamycin<br />resistant plates. |
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!Construct | !Construct | ||
- | !Sequencing <br /> Result | + | !Sequencing<br />Result |
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|J23113 + B0034 + C0051 | |J23113 + B0034 + C0051 | ||
|Good | |Good |
Revision as of 17:50, 13 July 2011
Tuesday, July 5
Clone OR and CRO
Protocol | Part Involved with Protocol | |
---|---|---|
Restriction Digest | Inserts using XbaI and PstI: | OR CRO |
Vector using XbaI and PstI: | K3 | |
Ligation | K3 + OR K3 + CRO | |
Transformation/Plating | The above ligations were transformed into Escherichia coli cells and plated onto kanamycin resistant plates. |
cI Repressor Test Assembly, Day 27
Sequencing Results: The following constructs were sent to sequencing to verify their DNA sequences:
Construct | Sequencing Result |
---|---|
J23113 + B0034 + C0051 | Good |
KOR + K648000 | Good |