Sensor: Week 7 June 28-July 1

From 2011.igem.org

Revision as of 14:40, 6 July 2011 by Ajb5663 (Talk | contribs)

Tuesday, June 28

cI Repressor Test Assembly, Day 23

     The sensor group continued their assembly of their testing construct. They placed an RBS in the K3 vector with Cro, the OR in front of the mCherry reporter, terminator, cI repressor and J23113 promoter as well as in front of the 34mCherry. Finally, the sensor group attempted (again) to put the B0032 RBS in front of the cI repressor.

Protocol Part Involved with Protocol
Restriction Digest Inserts using XbaI and PstI: 34mCherry
K648000+K648001
Inserts using EcoRI and SpeI: B0032
B0034
Vectors using EcoRI and XbaI: C0051
K3+Cro
Vector using SpeI and PstI: K3+OR
Ligation B0034 + K3 + Cro
KOr + K648000 + K648001
KOr + 34mcherry
B0032 + C0051
Transformation/Plating The above ligations were transformed into
Escherichia coli cells and plated on kanamycin
resistant plates.

     The sensor group also transformed K3+Cro and J23113+B0031+C0051 in order to make stocks. The B0034+C0051 was grown up from stock.



Back to Notebook