Reporter: Week 7 June 26-July 1

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Sunday, June 26

Insert tev Protease into K3 Vector, Take 7 Day 1

     The plates containing the assembly from 6/25 grew colonies that only contained RFP, none of which contained the tev protease. Thus, this assembly must be done again.

     The purified tev protease PCR product from 6/4 and the K3 miniprep from 6/25 were digested with XbaI and PstI in buffer 3. The digests were ligated together, then the ligation was transformed into competent Escherichia coli cells and plated onto kanamycin resistant plates.

Monday, June 27