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From 2011.igem.org

Contents 1 Sunday, July 3 1.1 Insert RecAI into K3 Vector, Take 3 Day 3 2 Tuesday, July 5 2.1 RecAI Mutagenesis Test Take 4, Day 1

Sunday, July 3

Insert RecAI into K3 Vector, Take 3 Day 3

 nbsp;nbsp;nbsp;nbsp;The K3 plasmid was extracted using the Omega Bio-Tek miniprep kit. The plasmid was digested with XbaI and SpeI. No band showed on an agarose gel, indicating the lack of DNA. The assembly could not be performed until the plasmid extraction problems get fixed. The RecAI PCR products were prepared from genomic DNA.

Tuesday, July 5

RecAI Mutagenesis Test Take 4, Day 1

nbsp;nbsp;nbsp;nbsp;nbsp;The terminator (B0015) and the cI reporter (I763007)

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