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RecA: Week 5, June 13-18
From 2011.igem.org
Monday
RecAI Mutagenesis Test, Take 2 Day 6
No colonies grew over the weekend. Thus, the PcI reporter (I763007) was assembled to the terminator (B0015) through amplified insert assembly. The assembly hit a road block at the plating stage because no agar plates existed in the lab. Thus, the RecA group attempted to make plates themselves. The transformed cells were grown in culture, but the recovery media with the cells were kept in order to plate the next day, once more plates were made.
The testing construct will be able to use either part K648001 or K648002 parts, which were assembled and sequenced by the sensor group. These parts feature the constitutive promoter in front of the cI represser, which is necessary for the RecA1 mutagenesis testing construct.
