File:20110710 optGenes+C3.jpg

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(<html> Gel of transformants with synthesized <i>dspB</i> and <i>esp</i> with codons optimized for expression in <i>Caulobacter</i> after ligation into pSB1C3. Lane 1: ladder; lanes 2-5: optimized <i>dspB</i>; lanes 6-9: optimized <i>esp</i>. Lane 4 shows )
 
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Gel of transformants with synthesized <i>dspB</i> and <i>esp</i> with codons optimized for expression in <i>Caulobacter</i> after ligation into pSB1C3. Lane 1: ladder; lanes 2-5: optimized <i>dspB</i>; lanes 6-9: optimized <i>esp</i>. Lane 4 shows successful insertion of <i>dspB</i> into pSB1C3, but none of the optimized <i>esp</i> transformants show the desired insert.
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Figure 5: Optimized dspB transformed into pSB1C3 colonies A-E. Lane 1: ladder; lanes 2-5: optimized dspB colonies A-E; lanes 6-9: optimized esp. Colony D shows the expected size.  
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Latest revision as of 14:04, 5 August 2011

Figure 5: Optimized dspB transformed into pSB1C3 colonies A-E. Lane 1: ladder; lanes 2-5: optimized dspB colonies A-E; lanes 6-9: optimized esp. Colony D shows the expected size.

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current16:26, 12 July 2011Thumbnail for version as of 16:26, 12 July 2011450×440 (8 KB)Aaring (Talk | contribs) (<html> Gel of transformants with synthesized <i>dspB</i> and <i>esp</i> with codons optimized for expression in <i>Caulobacter</i> after ligation into pSB1C3. Lane 1: ladder; lanes 2-5: optimized <i>dspB</i>; lanes 6-9: optimized <i>esp</i>. Lane 4 shows )

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