Copenhagen/6 July 2011

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Revision as of 11:20, 6 July 2011 by EvaCar (Talk | contribs)

Wednesday

Today we will make our first biobrick with the CYP 79A1 and finish the mutagenesis started yesterday.


Lab Work

  • Digestion of our PCR products - see protocols Mutations
  • Transformation of E.coli cells with the mutant plasmids - see protocol Transformations
  • PCR on the purified mutant 79A1 CYP. By using our designed primers this will insert the suffix and prefix at each end of the CYP79A1, thus making it a biobrick.


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