Copenhagen/6 July 2011

From 2011.igem.org

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=='''Wednesday'''==
=='''Wednesday'''==
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Today we will make our first biobrick with the CYP 79A1 and finish the mutagenesis started yesterday. 
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===Lab Work===
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* Digestion of our PCR products  - see protocol [[Team:Copenhagen/Protocol#Mutations|Mutations]]
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* Transformation of E.coli cells with the mutant plasmids - see protocol [[Team:Copenhagen/Protocol#Transformations|Transformations]]
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* [[Team:Copenhagen/Protocol#PCR_Reaction|PCR]] on the purified mutant 79A1 CYP. By using our designed primers this will insert the suffix and prefix at each end of the CYP79A1, thus making it a biobrick.
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===Other Work===
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*We have send out e-mails to danish biotech companies in hope to achieve economical support.
Back to [[Team:Copenhagen/Notebook|Notebook]]
Back to [[Team:Copenhagen/Notebook|Notebook]]

Latest revision as of 12:33, 7 July 2011

Wednesday

Today we will make our first biobrick with the CYP 79A1 and finish the mutagenesis started yesterday.


Lab Work

  • Digestion of our PCR products - see protocol Mutations
  • Transformation of E.coli cells with the mutant plasmids - see protocol Transformations
  • PCR on the purified mutant 79A1 CYP. By using our designed primers this will insert the suffix and prefix at each end of the CYP79A1, thus making it a biobrick.


Other Work

  • We have send out e-mails to danish biotech companies in hope to achieve economical support.

Back to Notebook