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Copenhagen/6 July 2011
From 2011.igem.org
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===Lab Work=== | ===Lab Work=== | ||
| - | * Digestion of our PCR products - see | + | * Digestion of our PCR products - see protocol [[Team:Copenhagen/Protocol#Mutations|Mutations]] |
| - | * Transformation of E.coli cells with the mutant plasmids - see protocol [[Team:Copenhagen/Protocol | + | * Transformation of E.coli cells with the mutant plasmids - see protocol [[Team:Copenhagen/Protocol#Transformations|Transformations]] |
| - | * PCR on the purified mutant 79A1 CYP. By using our designed primers this will insert the suffix and prefix at each end of the CYP79A1, thus making it a biobrick. | + | * [[Team:Copenhagen/Protocol#PCR_Reaction|PCR]] on the purified mutant 79A1 CYP. By using our designed primers this will insert the suffix and prefix at each end of the CYP79A1, thus making it a biobrick. |
Back to [[Team:Copenhagen/Notebook|Notebook]] | Back to [[Team:Copenhagen/Notebook|Notebook]] | ||
Revision as of 12:24, 6 July 2011
Wednesday
Today we will make our first biobrick with the CYP 79A1 and finish the mutagenesis started yesterday.
Lab Work
- Digestion of our PCR products - see protocol Mutations
- Transformation of E.coli cells with the mutant plasmids - see protocol Transformations
- PCR on the purified mutant 79A1 CYP. By using our designed primers this will insert the suffix and prefix at each end of the CYP79A1, thus making it a biobrick.
Back to Notebook
