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Copenhagen/6 July 2011
From 2011.igem.org
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=='''Wednesday'''== | =='''Wednesday'''== | ||
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Today we will make our first biobrick with the CYP 79A1 and finish the mutagenesis started yesterday. | Today we will make our first biobrick with the CYP 79A1 and finish the mutagenesis started yesterday. | ||
| + | |||
'''Labwork''' | '''Labwork''' | ||
| - | * Digestion of our PCR products | + | * Digestion of our PCR products - see protocols [[Team:Copenhagen/Protocol#Mutations|Mutations]] |
* Transformation of E.coli cells with the mutant plasmids - see protocol [[Team:Copenhagen/Protocol##Transformations|Transformations]] | * Transformation of E.coli cells with the mutant plasmids - see protocol [[Team:Copenhagen/Protocol##Transformations|Transformations]] | ||
Revision as of 11:19, 6 July 2011
Wednesday
Today we will make our first biobrick with the CYP 79A1 and finish the mutagenesis started yesterday.
Labwork
- Digestion of our PCR products - see protocols Mutations
- Transformation of E.coli cells with the mutant plasmids - see protocol Transformations
- PCR on the purified mutant 79A1 CYP. By using our designed primers this will insert the suffix and prefix at each end of the CYP79A1, thus making it a biobrick.
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