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Copenhagen/5 July 2011
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* Transformed ''XL1-Blue'' competent cells - see protocol [[Team:Copenhagen/Protocol##Transformations|Transformations]] | * Transformed ''XL1-Blue'' competent cells - see protocol [[Team:Copenhagen/Protocol##Transformations|Transformations]] | ||
| - | * We did a [[Team:Copenhagen/Protocol#Mini_prep|Mini prep]] on the A1 overnight culture from yesterday. We cut the plasmids with PstI-HF and ran a gel with the resulting DNA. Great Succes!!! Only one band appeared, indicating that we succesfully did a point mutation in the target PstI restriction site. The DNA has now been sent to sequencing and expect the answer in two or three days. | + | * We did a [[Team:Copenhagen/Protocol#Mini_prep|Mini prep]] on the A1 overnight culture from yesterday. We [[Team:Copenhagen/Protocol#Restrictionsite_analysis|cut]] the plasmids with PstI-HF and ran a gel with the resulting DNA. Great Succes!!! Only one band appeared, indicating that we succesfully did a point mutation in the target PstI restriction site. The DNA has now been sent to sequencing and expect the answer in two or three days. |
[[Image:A1.jpg|230px|left]] | [[Image:A1.jpg|230px|left]] | ||
Latest revision as of 11:32, 13 July 2011
Tuesday
We are two men down today. One with fewer and one with a sprained foot. But the sun is shining and the spirit is high!
Lab Work
- There were no colonies on the plates from yesterday. We checked all of our calculations and made some corrections.
- We did mutations again, with the corrections. Afterwards we digested our mutants with Dnp - see protocols Mutations
- Transformed XL1-Blue competent cells - see protocol Transformations
- We did a Mini prep on the A1 overnight culture from yesterday. We cut the plasmids with PstI-HF and ran a gel with the resulting DNA. Great Succes!!! Only one band appeared, indicating that we succesfully did a point mutation in the target PstI restriction site. The DNA has now been sent to sequencing and expect the answer in two or three days.
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