Copenhagen/4 July 2011

From 2011.igem.org

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===Lab Work===
===Lab Work===
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* On the A1 plate from friday, there were one colony. Big succes. We made an overnightculture.
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* We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out.
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* We checked our [[Team:Copenhagen/Protocol#Restrictionsite_analysis|restrictiondigest]] of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out.
* We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program.  
* We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program.  
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Afterwards we digested our mutants with Dnp [[Team:Copenhagen/Protocol#Mutations|Mutations]]  
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Afterwards we digested our mutants with Dnp - see protocols [[Team:Copenhagen/Protocol#Mutations|Mutations]]
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* Transformed ''XL1-Blue'' competent cells - see protocol [[Team:Copenhagen/Protocol##Transformations|Transformations]]  
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* Transformed ''XL1-Blue'' competent cells - protocols  [[Team:Copenhagen/Protocol##Transformations|Transformations]]
 
Back to [[Team:Copenhagen/Notebook|Notebook]]
Back to [[Team:Copenhagen/Notebook|Notebook]]

Latest revision as of 11:31, 13 July 2011

Monday

The rest of the team have returned, happy, well rested and eager to begin working.

Lab Work

  • On the A1 plate from friday, there were one colony. Big succes. We made an overnightculture.
  • We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out.
  • We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program.

Afterwards we digested our mutants with Dnp - see protocols Mutations


Back to Notebook