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Copenhagen/4 July 2011
From 2011.igem.org
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* We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out. | * We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out. | ||
| - | * We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program. | + | * We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program.t |
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| + | * We digested our mutants with Dnp and transformed ''XL1-Blue'' competent cells - protocols [[Team:Copenhagen/Protolcol]] | ||
Back to [[Team:Copenhagen/Notebook|Notebook]] | Back to [[Team:Copenhagen/Notebook|Notebook]] | ||
Revision as of 12:15, 4 July 2011
Monday
The rest of the team have returned, happy, well rested and eager to begin working.
Lab Work
- We checked our restrictiondigest of the alledged mutants but there was nothing to see on the gel and we have given up and thrown these "mutants" out.
- We did mutations again, with small alterations of the amounts of dNTP mix and polymerase and an altered PCR program.t
- We digested our mutants with Dnp and transformed XL1-Blue competent cells - protocols Team:Copenhagen/Protolcol
Back to Notebook
