Copenhagen/29 August 2011

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Monday

Labwork

  • PCR purification of B1 after prefix-suffix-PCR. The purifactions are Nanodropped - B1 samples 3,4,5,8 and 10 looks fine.
  • Digestions (1 h by 37 degrees or O/N)
* PSB1C3 with E + P
* A2 in PSB1C3 with X + P
* Expr.vector with P + S
* B1 3,4,5,7 and 10 with E + P
  • Purification of the digestions by using the PCR purifaction kit
  • Ligations
* PSB1C3 + B1 samples 3,4,5,8 and 10
* Expr. vector + A2
* Selfmade Expr. vector + A2
  • Transformation of ligations
  • Made O/N cultures on: 2C9, UserB1 to Sequencing, UserB1 to growth and Control BL21 cells to growth.
  • Made TB media and autoclaved bottles for tomorrow.
  • Made Cam + IPTG plates


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