Copenhagen/29 August 2011

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(Created page with "==Monday== '''Labwork''' * PCR purification of B1 after prefix-suffix-PCR. The purifactions are Nanodropped - B1 samples 3,4,5,8 and 10 looks fine. * Digestions (1 h by 37 de...")
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* Digestions (1 h by 37 degrees or O/N)
* Digestions (1 h by 37 degrees or O/N)
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* PSB1C3 with E + P
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PSB1C3 with E + P
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* A2 in PSB1C3 with X + P
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* Expr.vector with P + S
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A2 in PSB1C3 with X + P
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* B1 3,4,5,7 and 10 with E + P
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+
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Expr.vector with P + S
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+
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B1 3,4,5,7 and 10 with E + P
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* Purification of the digestions by using the PCR purifaction kit
* Purification of the digestions by using the PCR purifaction kit
* Ligations
* Ligations
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* PSB1C3 + B1 samples 3,4,5,8 and 10
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PSB1C3 + B1 samples 3,4,5,8 and 10
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* Expr. vector + A2
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* Selfmade Expr. vector + A2
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Expr. vector + A2
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Selfmade Expr. vector + A2
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* Transformation of ligations
* Transformation of ligations
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* Made Cam + IPTG plates  
* Made Cam + IPTG plates  
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* Made plates with fungi and oximes, just to try it in another way. Put it in the 37 degrees incubator.
Back to [[Team:Copenhagen/Notebook|notebook]]
Back to [[Team:Copenhagen/Notebook|notebook]]

Latest revision as of 15:47, 30 August 2011

Monday

Labwork

  • PCR purification of B1 after prefix-suffix-PCR. The purifactions are Nanodropped - B1 samples 3,4,5,8 and 10 looks fine.
  • Digestions (1 h by 37 degrees or O/N)
* PSB1C3 with E + P
* A2 in PSB1C3 with X + P
* Expr.vector with P + S
* B1 3,4,5,7 and 10 with E + P
  • Purification of the digestions by using the PCR purifaction kit
  • Ligations
* PSB1C3 + B1 samples 3,4,5,8 and 10
* Expr. vector + A2
* Selfmade Expr. vector + A2
  • Transformation of ligations
  • Made O/N cultures on: 2C9, UserB1 to Sequencing, UserB1 to growth and Control BL21 cells to growth.
  • Made TB media and autoclaved bottles for tomorrow.
  • Made Cam + IPTG plates
  • Made plates with fungi and oximes, just to try it in another way. Put it in the 37 degrees incubator.


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