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From 2011.igem.org

Monday

This morning we have found out that the pcr machine we used thursday is broken, so that can be the explanation for why the results friday were bad.

Labwork

• Do the pcr again and test on a gel

• Digested psb1c3

• Transformed A2-1 and A2-2 from the ligation thursday into psb1c3

• Transformed B1 into the expression vector

• Made an overnight culture on the colonies we did the pcr on, because it again didn't look as we hoped, so we will do at plasmid prep to test if the ligation is there anyway

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