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From 2011.igem.org

Thursday

Today started out fantastic! The transformations of A1 in the expression vector had been very succesful, as there were plenty of colonies on the dish. An event we haven't been used to... Our spirits raised even higher when we realised that there was a single colony on the plate where A1 had been transformed with pSB1C3 and six colonies on the plate for A2. This has so far been somewhat of a challenge! We cross fingers that indeed the A1 and A2 biobricks are ligated into the plasmid. Finally, Maria showed us an overnight culture of E.coli with the pSB1C3 plasmid, which had turned all red. This made the whole group go into complete ecstasy and the rest of the day has been one big smile! (Besides the fact that we did not succeed transforming B1 in pSB1C3 into E.coli, but this only means that we also have had work to do today!)

A1

• Colonies with either pSB1C3 or the expression vector, have been plated and a PCR was performed to check whether there was insert in them or not.

A2

• Colonies with either pSB1C3 or the expression vector, have been plated and a PCR was performed to check whether there was insert in them or not. We are uncertain if we have followed the protocols correctly for this biobrick.

This is the reason why we have performed an other prefix and suffix PCR, purified, double digested and ligated A2 again, and now transformed it.

B1

• We have been adding prefix and suffix again, purified, double digested and ligated it into pSB1C3 and the expression vector.

Other Work

• Our ethics discussion document has been sent to Sune Holm for comments and corrections. We hope to get a nice respond as soon as possible in order to send it out to professors and other staff at Biocenter and at KU-LIFE.

• We have written to Colombia team (and not ColUmbia, as we unfortunatedly wrote in the email)as they work on a project that invovles fungi and a fungi specific inducible promotor, just like the one that would fit our project just perfect.

• We got a contact from our friends at DTU, Jens Something, and we have now written and asked him for help on a fungi inducible promotor.

• Team building: Playing awesome games, such as Tegn og Gæt and Bezzerwizzer. Yihaaa!

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