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From 2011.igem.org

Thursday

Today started out fantastic! The transformations of A1 in the expression vector had been very succesful, as there were plenty of cultures on the dish. An event we haven't been used to... Our spirits raised even higher when we realised that there was a single colony on the plate where A2 had been transformed with pSB1C3. This has so far been somewhat of a challenge! We cross fingers that indeed the A2 biobrick is ligated into the plasmid. Finally, Maria showed us an overnight culture of E.coli with the pSB1C3 plasmid, which had turned all red. This made the whole group go into complete ecstasy and the rest of the day has been one big smile! (Besides the fact that we did not succeed transforming either A1 or B1 in pSB1C3 into E.coli, but this only means that we also have had work to do today!)