Copenhagen/16 August 2011

From 2011.igem.org

Revision as of 07:54, 17 August 2011 by EvaCar (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Tuesday

Labwork

Checked the TLC from yesterday, the result were not good. There were no oxime bands where we've added either tyrosine or phenylalanine as substrate to A1 and A2.

User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again.

Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1, 2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow. See protocol Expression & Purification in BL21

Other work

Worked on the poster

Worked on the illustrations

Searched for hotel in Amsterdam

Back to Notebook

Retrieved from "http://2011.igem.org/Copenhagen/16_August_2011"