Copenhagen/16 August 2011

From 2011.igem.org

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(Tuesday)
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* User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again.
* User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again.
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* Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1,2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow.   [[Team:Copenhagen/Protocol#Expression & Purification in BL21
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* Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1,2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow.
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|Expression & Purification in BL21]]
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[[Team:Copenhagen/Protocol#Expression & Purification in BL21|Expression & Purification in BL21]]

Revision as of 12:13, 16 August 2011

Tuesday

Labwork

  • Checked the TLC from yesterday, the result was not good. There was no oxime bands where we've added either tyrosine or phenylalanine as substrate to A1 and A2.
  • User assembly: Checked the plates from yesterday, there were no colonies. We've contacted DTU to get some more DNA, enabling us to try again.
  • Transfer overnight culture to TB media, growth until OD600 = 0,5. Added IPTG and 5-aminolevulinic acid hydrochloride, and moved the bottle to 28 degrees incubator. Took samples 1,2 and 4 hours after the addition of IPTG and prepared samples for SDS-PAGE tomorrow.

Expression & Purification in BL21



Other work

  • Worked on the poster
  • Worked on the illustrations
  • Searched for hotel in Amsterdam


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