Chloramphenicol stock

From 2011.igem.org

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(Created page with "Materials: Chloramphenicol powder ddH2O sterile syringe syringe filter sterile 15ml centrifuge tube Procedure: 1. Weigh the appropriate amount of antibiotic powder for the amo...")
 
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Materials:
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'''Materials:'''
Chloramphenicol powder
Chloramphenicol powder
 +
ddH2O
ddH2O
 +
sterile syringe
sterile syringe
 +
syringe filter
syringe filter
 +
sterile 15ml centrifuge tube
sterile 15ml centrifuge tube
-
Procedure:
+
'''Procedure:'''
-
1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (we use 0.165g in 5 ml of H2O to make a 33mg/ml solution.)
+
1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (''we use 0.165g in 5 ml of H2O to make a 33mg/ml solution.'')
2. Mix your antibiotic with your ddH2O
2. Mix your antibiotic with your ddH2O

Latest revision as of 20:40, 28 September 2011

Materials:

Chloramphenicol powder

ddH2O

sterile syringe

syringe filter

sterile 15ml centrifuge tube

Procedure:

1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (we use 0.165g in 5 ml of H2O to make a 33mg/ml solution.)

2. Mix your antibiotic with your ddH2O

3. Absorb your solution with the sterile syringe.

4. Place the syringe filter onto the syringe.

5. Filter your solution into the sterile 15ml centrifuge tube.

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