Agenda: Transforming Top 10 cells to Establish Hg sensitive promoter part K346002

From 2011.igem.org

05/26/11 Agenda: Transforming Top 10 cells to Establish Hg sensitive promoter part K346002

(1) 5 microliters of transformation media (BIO RAD Catalogue 166-0409 received 1-17-11 GT) 
    added to 40 microliters of competent cells in LB broth.
(2) 2 microliters of K346002 DNA added to broth.
(3) Solution was placed in ice bath for 5 minutes.
(4) Placed in 44.7 degree Celsius water for 50 seconds.
(5) Placed in ice bath for 5 minutes.
(6) 1 microliter of SOB buffer at rrom temperature added to broth 
   (in order to close plasmid channels). 
(7) PLated 100 microliters of cells on LB AMP plates (5-26-11 AM). 
Expected: 10 to 50 colonies projected to grow.


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