Agenda: Examining Results of Establishment of the K346002 Hg Sensitive Promotor Round II

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05/26/11 Agenda: Examining Results of Establishment of the K346002 Hg Sensitive Promotor Round II

(1) Results: NO GROWTH!
(2) Conclusion: Perhaps no transformation and bad AMP on 4-21-10 plate. However, could still
    be some transformants present.
(3) Q-tip swabbed up lawn and resuspended in AMP LB broth. Resuspension fluid placed in 
    photospectrometer.
(4) Photospectrometer results for K346002:
Time         A600 nm
 0 hr.            .315
 1 hr.            .148
 2.5 hr.          .133
(5) Results: absorbance is declining meaning cells are dying, thus no transformation 
    has resulted. Will leave in incubator overnight to see if transformants grow out.


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