"
Agenda: Examining Results of Establishment of the K346002 Hg Sensitive Promotor Round II
From 2011.igem.org
(Difference between revisions)
(Created page with " {| style="color:#008080;background-color:#7CFC00;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" !align="center"|[[Team:SouthBend-Mi...") |
|||
| Line 1: | Line 1: | ||
| + | ===''' 05/26/11 Agenda: Examining Results of Establishment of the K346002 Hg Sensitive Promotor Round II'''=== | ||
| + | (1) Results: NO GROWTH! | ||
| + | |||
| + | (2) Conclusion: Perhaps no transformation and bad AMP on 4-21-10 plate. However, could still | ||
| + | be some transformants present. | ||
| + | |||
| + | (3) Q-tip swabbed up lawn and resuspended in AMP LB broth. Resuspension fluid placed in | ||
| + | photospectrometer. | ||
| + | |||
| + | (4) Photospectrometer results for K346002: | ||
| + | |||
| + | <u>Time</u> <u>A600 nm</u> | ||
| + | 0 hr. .315 | ||
| + | 1 hr. .148 | ||
| + | 2.5 hr. .133 | ||
| + | |||
| + | (5) Results: absorbance is declining meaning cells are dying, thus no transformation | ||
| + | has resulted. Will leave in incubator overnight to see if transformants grow out. | ||
Latest revision as of 16:45, 4 June 2011
05/26/11 Agenda: Examining Results of Establishment of the K346002 Hg Sensitive Promotor Round II
(1) Results: NO GROWTH!
(2) Conclusion: Perhaps no transformation and bad AMP on 4-21-10 plate. However, could still
be some transformants present.
(3) Q-tip swabbed up lawn and resuspended in AMP LB broth. Resuspension fluid placed in
photospectrometer.
(4) Photospectrometer results for K346002:
Time A600 nm 0 hr. .315 1 hr. .148 2.5 hr. .133
(5) Results: absorbance is declining meaning cells are dying, thus no transformation
has resulted. Will leave in incubator overnight to see if transformants grow out.
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
