7 (June 7, 2011 GDS)

From 2011.igem.org

-Spin 1.5 mL of overnight culture in microfuge </br> -Aspirate all but 100uL of the supernatant and resuspend pellet by vortexing</br> -Add 300uL of TENS and mix by inversion</br> -Add 150uL of sodium acetate and vortex</br> -Centrifuge for 2.5 minutes at 10K</br> -Transfer supernatant to clean tube and add 1mL of room temp. ETOH</br> -Mix and pellet DNA by centrifuge for 2-5 min. at 10K</br> -Wash pellet with 70% ethanol and allow pellet to dry</br> -Resuspend pellet in 30uL of TE w/ RNA seA</br> -Digest 5-10uL as usual</br>

To make TENS, add:

-4.5mL of TE

-250uL 10%SDS

-250uL 2N NaOH