7 (June 7, 2011 GDS)

From 2011.igem.org

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-Spin 1.5 mL of overnight culture in microfuge
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<gallery widths=910px heights=800px>
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File:June7GDS.jpg
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</gallery>
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-Aspirate all but 100uL of the supernatant and resuspend pellet by vortexing
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<div class="center" style="width:auto; margin-left:auto; margin-right:auto;">[[Image:gelpicnewerGDS.jpg|425px]]
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</div>
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-Add 300uL of TENS and mix by inversion
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-Add 150uL of sodium acetate and vortex
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-Centrifuge for 2.5 minutes at 10K</br>
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-Transfer supernatant to clean tube and add 1mL of room temp. ETOH
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-Mix and pellet DNA by centrifuge for 2-5 min. at 10K
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-Wash pellet with 70% ethanol and allow pellet to dry
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-Resuspend pellet in 30uL of TE w/ RNA seA
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-Digest 5-10uL as usual
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:To make TENS, add:
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::-4.5mL of TE
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:::-250uL 10%SDS
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:::-250uL 2N NaOH
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Latest revision as of 20:43, 28 September 2011

GelpicnewerGDS.jpg