27 (June 27, 2011 GDS)

From 2011.igem.org

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<gallery widths=910px heights=800px>
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File:June232427GDS.jpg
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'''Safety:'''
'''Safety:'''
''Initial Bleach Kill Curve''
''Initial Bleach Kill Curve''
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Sample Bacteria for OD and initial cell number
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<gallery widths=910px heights=800px>
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:-log phase culture  OD= .194
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File:SJune27GDS.jpg
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:-stationary phase culture OD= .685
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</gallery>
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:- No cell count for either (too many)
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Experiment
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-Mix 4.5 mL culture with 500&lambda; bleach
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-Incubate for 15 minutes and plate 1 mL
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-Collect 1 mL and spin to pellet
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-Wash twice with TE and resuspend in 100&lambda; LB and then plate
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:- Result: No Colonies
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Note: LB plates had bright pink colonies
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Latest revision as of 15:50, 24 September 2011

Safety:

Initial Bleach Kill Curve