26 (May 26, 2011 GDS)

From 2011.igem.org

(Difference between revisions)
m (spelling)
Line 10: Line 10:
:-Add  
:-Add  
::300uL TENS:    mix
::300uL TENS:    mix
-
::150uL NaOC:    mix
+
::150uL NaOH:    mix
:-Spin 2.5'
:-Spin 2.5'
:-Transfer supernatant to clean tube and add 1mL of EtOH
:-Transfer supernatant to clean tube and add 1mL of EtOH
:-Vortex and pellet DNA by centrifugation for 2.5mL
:-Vortex and pellet DNA by centrifugation for 2.5mL

Revision as of 20:44, 6 September 2011

Created TENS

4.5 mL TE and
250uL 10% SDS
250uL 2N NaOH


-Spin 4 tubes (1.5mL) 30"
-Pour off sol'n & resuspend
-Add
300uL TENS: mix
150uL NaOH: mix
-Spin 2.5'
-Transfer supernatant to clean tube and add 1mL of EtOH
-Vortex and pellet DNA by centrifugation for 2.5mL