18 (May 18, 2011 GDS)

From 2011.igem.org

(Difference between revisions)
m (grammar)
Line 20: Line 20:
:pNICE SA-15λ TE
:pNICE SA-15λ TE
:J024430 GE-15λ TE
:J024430 GE-15λ TE
-
:Trans002-5, AB-Resp 30&lambda
+
:Trans002-5, AB-Resp 30λ
:Trans003-W1 SD, SA-
:Trans003-W1 SD, SA-

Revision as of 20:41, 6 September 2011

Parth--made fresh TENS

4.5 TE
250λ 10% SDS
250λ 2M NaOH


Spin 1.5 mL (2 tubes) 30"
Pour off sol'n + resuspend
Add: 300λ TENS mix
150λ NaOAc mix
Spin 2.5'
Sup to new tube
Add 1 mL ETOH - mix well
Spin 3' ________
Wash 70% ETOH


Minipreps (2 each) for:
pNICE SA-15λ TE
J024430 GE-15λ TE
Trans002-5, AB-Resp 30λ
Trans003-W1 SD, SA-